全文获取类型
收费全文 | 918篇 |
免费 | 22篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 19篇 |
儿科学 | 2篇 |
妇产科学 | 4篇 |
基础医学 | 139篇 |
口腔科学 | 99篇 |
临床医学 | 49篇 |
内科学 | 156篇 |
皮肤病学 | 2篇 |
神经病学 | 86篇 |
特种医学 | 31篇 |
外科学 | 133篇 |
综合类 | 61篇 |
预防医学 | 76篇 |
眼科学 | 3篇 |
药学 | 65篇 |
中国医学 | 3篇 |
肿瘤学 | 24篇 |
出版年
2023年 | 7篇 |
2022年 | 13篇 |
2021年 | 13篇 |
2020年 | 30篇 |
2019年 | 16篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 13篇 |
2015年 | 15篇 |
2014年 | 50篇 |
2013年 | 52篇 |
2012年 | 26篇 |
2011年 | 35篇 |
2010年 | 36篇 |
2009年 | 38篇 |
2008年 | 42篇 |
2007年 | 44篇 |
2006年 | 39篇 |
2005年 | 36篇 |
2004年 | 48篇 |
2003年 | 33篇 |
2002年 | 20篇 |
2001年 | 17篇 |
2000年 | 17篇 |
1999年 | 14篇 |
1998年 | 24篇 |
1997年 | 16篇 |
1996年 | 15篇 |
1995年 | 17篇 |
1994年 | 19篇 |
1993年 | 18篇 |
1992年 | 18篇 |
1991年 | 10篇 |
1990年 | 8篇 |
1989年 | 12篇 |
1988年 | 14篇 |
1987年 | 12篇 |
1986年 | 7篇 |
1985年 | 13篇 |
1984年 | 10篇 |
1983年 | 10篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1978年 | 7篇 |
1977年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有952条查询结果,搜索用时 31 毫秒
1.
《Vaccine》2022,40(7):1001-1009
Vaccination guidelines for dogs and cats indicate that core vaccines (for dogs, rabies, distemper, adenovirus, parvovirus; for cats, feline parvovirus, herpes virus-1, calicivirus) are essential to maintain health, and that non-core vaccines be administered according to a clinician’s assessment of a pet’s risk of exposure and susceptibility to infection. A reliance on individual risk assessment introduces the potential for between-practice inconsistencies in non-core vaccine recommendations. A study was initiated to determine non-core vaccination rates of dogs (Leptospira, Borrelia burgdorferi, Bordetella bronchiseptica, canine influenza virus) and cats (feline leukemia virus) in patients current for core vaccines in veterinary practices across the United States. Transactional data for 5,531,866 dogs (1,670 practices) and 1,914,373 cats (1,661 practices) were retrieved from practice management systems for the period November 1, 2016 through January 1, 2020, deidentified and normalized. Non-core vaccination status was evaluated in 2,798,875 dogs and 788,772 cats that were core-vaccine current. Nationally, median clinic vaccination rates for dogs were highest for leptospirosis (70.5%) and B. bronchiseptica (68.7%), and much lower for canine influenza (4.8%). In Lyme-endemic states, the median clinic borreliosis vaccination rate was 51.8%. Feline leukemia median clinic vaccination rates were low for adult cats (34.6%) and for kittens and 1-year old cats (36.8%). Individual clinic vaccination rates ranged from 0 to 100% for leptospirosis, B. bronchiseptica and feline leukemia, 0–96% for canine influenza, and 0–94% for borreliosis. Wide variation in non-core vaccination rates between clinics in similar geographies indicates that factors other than disease risk are driving the use of non-core vaccines in pet dogs and cats, highlighting a need for veterinary practices to address gaps in patient protection. Failure to implement effective non-core vaccination strategies leaves susceptible dogs and cats unprotected against vaccine-preventable diseases. 相似文献
2.
《Vaccine》2020,38(2):115-118
Different strategies have been proposed to overcome maternally derived antibody (MDA) interference with canine parvovirus type 2 (CPV-2) immunisation, including intranasal vaccination, which presents some practical limitations. In the present study, the results of the oral administration of a commercial CPV-2b modified live virus (MLV) vaccine in pups with MDA are reported. The CPV-2b vaccine was orally administered to 14 6-week-old pups with a bait. Blood samples and rectal swabs were collected at different days post-vaccination (dpv) to determine CPV-2 antibody titres and DNA loads. Thirteen pups were positive to serological and virological tests after the first vaccination and one pup became positive after the second vaccine administration. The findings of this study suggest that systemic immunity against CPV-2 may be achieved by the use of an MLV CPV-2b vaccine administered orally even in the presence of MDA titres that usually interfere with vaccination. 相似文献
3.
目的探讨犬双肺序贯性移植时供肺的采取及受体移植手术技巧。方法犬16条,体重差异<5%的随机配对作为供受体进行双肺序贯移植实验共8次,移植右肺钳夹左房时采用了‘v’钳夹技术,并检测左肺及右肺术后0.5、1、2 h各进行血气分析、肺水含量及电镜下超微结构,了解术后肺功能。结果移植右肺时采取的‘v’型钳夹术良好地解决了钳夹左房时因回心血量受阻而引起的心功能不全。术后检测结果表明移植后肺功能良好。结论掌握了犬双肺序贯性移植的全过程,"V"形双阻断钳夹左心房技术提高了犬肺移植的成功率。 相似文献
4.
Canine babesiosis and canine ehrlichiosis are the major tick-borne diseases throughout the world. The concurrent infection
between canine babesiosis and canine ehrlichiosis can occur in endemic regions. This report showed the clinical pathology
and treatment outcome of concurrent infection in a dog admitted to a veterinary hospital. Based on the history, physical examination,
and laboratory finding, the dog was diagnosed with neurological disorders induced by babesiosis and ehrlichiosis concurrent
infection. The dog presented with moderate normocytic normochromic anemia, severe thrombocytopenia and moderately increased
alkaline phosphatase. The symptoms were more complicated with high fever, seizure, and cardiopulmonary arrest. The condition
was specifically treated with doxycycline and diminazene aceturate, but the dog died 2 days after admission. In conclusion,
concurrent infection of canine babesiosis and canine ehrlichiosis can cause complicated clinical neurological compromise and
result in mortality in dogs. 相似文献
5.
Canine Distemper Virus (CDV) produces an encephalitis in dogs that varies with viral strain. We have studied the cell tropisms of two virulent strains (CDV-SH and CDV A75-17) and an attenuated strain, Rockborn (CDV-RO), in cultured canine brain cells. Infected cell types were identified by double immunofluorescent labeling of specific cell markers and viral antigens. All viral strains studied produced infection in astrocytes, fibroblasts, and macrophages. Neurons were not infected by CDV A75-17 but were rapidly infected by CDV-SH and CDV-RO. Multipolar oligodendrocytes were very rarely infected by any of the virus strains. In contrast, a morphologically distinct subset of bipolar oligodendrocytes were commonly infected by CDV-SH and CDV-RO. The kinetics of infection in the astrocytes, oligodendrocytes, neurons, and macrophages varied between strains. Both CDV-SH and CDV-RO rapidly infected bipolar oligodendrocytes, astrocytes, neurons, and macrophages by 14 days post infection while infection by CDV A75-17 was delayed until after 28-35 days post infection. The differences in the growth kinetics and cell tropisms for some brain cells, exhibited by the three viral strains examined in this in vitro study, may relate to the different CNS symptoms that these strains produce in vivo. 相似文献
6.
7.
Ichiro Ikegaki Yoshio Suzuki Shin-ichi Satoh Toshio Asano Masato Shibuya Kenichiro Sugita 《Naunyn-Schmiedeberg's archives of pharmacology》1989,340(4):431-436
Summary The effects of calcitonin gene-related peptide (CGRP) on canine cerebral arteries and on vertebral blood flow were investigated in-vivo and in-vitro and the findings compared with the effects of vasoactive intestinal peptide (VIP) and substance P. Administration of CGRP into the vertebral artery caused a dose-dependent and long-lasting increase in blood flow. The in-vivo vasodilatory effects of substance P and VIP were short-lasting. CGRP (0.1 to 100 nmol/l) elicited a concentration-dependent relaxation of the isolated middle cerebral and basilar arteries when the tissues were precontracted by exposure to prostaglandin F2 (PGF2). This effect was not antagonized by propranolol, atropine, tetrodotoxin, (N-Ac-Tyr1, D-Phe2)-growth hormone-releasing factor(1–29)-NH2 or (D-Pro2, D-Trp7,9) substance P. CGRP also reduced concentration-dependently the contraction of cerebral arteries induced by KCl or 9,11-epithio-11,12-metano-thromboxane A2 (STXA2). Mechanical removal of the endothelium did not abolish the vasodilatory response to CGRP. In PGF2-contracted canine cerebral arteries, VIP (0.1 to 100 nmol/l) was less potent a vasodilator than CGRP. At low concentrations (0.01 to 1 nmol/l) substance P elicited a rapid and short-lasting relaxation, and in the absence of endothelium this relaxation disappeared. These findings are clear evidence that CGRP modulates vascular tone. 相似文献
8.
采用成年狗正常股动脉段6例,测定其拉伸后的压力一容积关系,并求出其顺应性。另取狗股狗股动脉段30例拉伸固定后,观测其形态结构变化。发现狗股动脉段拉伸后的P-V曲线可用抛物线来拟合,狗股动脉段拉伸15%后顺应性明显下降,其形态结构无明显改变,拉伸30%后才出现明显的结构改变。狗股动脉拉伸后顺应性变化的出现较形态结构变化早。 相似文献
9.
Anuchai Niwetpathomwat 《Comparative clinical pathology》2006,15(4):215-219
Doxycycline has a well known broad spectrum activity against bacteria and rickettsia, as well as Ehrlichia spp. However, the use of doxycycline for the treatment of concurrent ehrlichiosis and babesiosis has rarely been evaluated, especially in veterinary hospital populations. A retrospective study of 70 canine ehrlichiosis and 12 canine babesiosis concurrent infections from Out Patient Department patients at Chulalongkorn Small Animal Teaching Hospital, admitted during 2001–2003, were studied. The results showed a complete curative effect of doxycycline on both canine ehrlichiosis and canine babesiosis concurrent infections. The red blood cell indices after treatment were significantly higher in canine ehrlichiosis (P < 0.05). The platelet cell counts after treatment were significantly higher in concurrent canine ehrlichiosis and babesiosis infections (P < 0.05). Doxycycline can be recommended as the drug of choice for both canine ehrlichiosis and canine babesiosis and concurrent infections of both conditions in veterinary hospitals. 相似文献
10.
Paul S. Thorner Reuben Baumal Victor E. O. Valli Don Mahuran Paula M. Marrano Robert Jacobs 《Virchows Archiv : an international journal of pathology》1992,421(6):467-475
Summary Some patients with hereditary nephritis (HN) who have received a renal transplant have been shown to form antibody with specificity for the NC1 domain of collagen type IV, a major constituent of glomerular basement membranes (GBM). We attempted to duplicate this phenomenon in a family of dogs with X-linked HN, a model for human X-linked HN, by immunizing affected male dogs with normal dog NC1 domain. A collagenase digest was prepared from normal dog GBM, the NC1 domain was separated into dimer (50 kDa) and monomer (24 kDa and 26 kDa) components by SDS-PAGE, and injected into two affected male dogs. Antisera obtained from both dogs contained antibody which reacted with the NC1 domain of dog and human GBM by a plate-binding radioimmunoassay, bound to the dimer and 26 kDa monomer bands by Western blotting, and staining dog and human GBM by immunofluorescence (IF). The affected male dog antiserum reacted equally by radioimmunoassay with the NC1 domain isolated from GBM of unaffected, affected male, and carrier female dogs in the family with X-linked HN, and bound by Western blotting to dimers and the 26 kDa monomer band of the NC1 domain of GBM in each group of dogs. However, the affected male dog antiserum differentiated these dogs by IF; it produced global staining of GBM of unaffected dogs, failed to stain GBM of affected male dogs, and produced segmental staining of GBM of carrier female dogs. Absorption of the affected male dog antiserum with normal dog NC1 domain eliminated the staining of dog GBM by IF, whereas staining persisted after absorption with affected male dog NC1 domain. The abnormal staining patterns of GBM seen by IF in the affected male and carrier female dogs and the results of the absorption studies imply an abnormality of one or more determinants in the 26 kDa monomer band of the NC1 domain of their GBM. Amino acid sequencing of this band identified the 1(IV) chain of collagen type IV, a finding that has implications for the pathogenesis of canine X-linked HN. Absent and segmental staining respectively were also seen by IF in GBM of a male and female patient with HN, using the affected male dog antiserum. Thus, the results obtained in affected male and carrier female dogs with X-linked HN may also be relevant to patients with this disease. 相似文献