自体颅骨碎片及屑修补颅骨缺损(附60例报告)

应用自体颅骨碎片及骨屑对60例在造成颅骨缺损术同时行颅骨缺损修补术。缺损面积直径最小者2cm,最大者10cm。术后随访1个月至5年,病人颅骨缺损修补处外观正常,无凹陷,触之硬,与正常颅骨处类似。6个月拍X线片示颅骨修补处类似正常颅骨形态。     

广西蓝山工酒的微量元素与氨基酸含量分析

目的：了解广西蓝山药酒（GXLSML）中微量元素及氨基酸的含量,方法：应用等离子体发射光谱法测定GXLSML的微量元素含量及应用游离氨基酸自动分析法测定GXLSML中的氨基酸含量,结果：GXISML富含有锌（Zn),铁（Fe),镁（Mg）,钙（Ca),铜（Cu),磷（P）,硒（Ce),锗（Ge)等人体必需的微量元素,GXLSML含17种以上氨基酸,其中7种以人体必需氨基酸,2种为人体半必需氨基酸,GXLSML中总氨基酸含量为17.18mg/10ml,其中必需氨基酸和半需氨基酸含量占总氨基酸的43％,结论：GXLSML含有多种人体必需的微量元素和氨基酸,利用人体的保健作用。     

抗脑抗体对精神分裂症自身免疫反应的探讨

目的探讨精神分裂症患者脑脊液中抗脑抗体(ABAb)的致病作用。方法应用ELISA法检测患者脑脊液抗脑抗体，并对患者尸解脑组织进行免疫组化染色和病理学检验。结果患者脑脊液中有73．9％(34／46)检出抗脑抗体；在脑右侧扣带回、海马及胼胝体等脑组织神经元细胞胞浆中发现IgG和补体C3沉着；脑组织呈现明显的多种退行性改变，伴胶质细胞增生。结论抗脑抗体可能参与精神分裂症自身免疫反应，使患者脑内某些部位出现自身抗体沉着并引起补体活化，而导致脑组织病理性改变。     

RB but not R‐HCVAD is a feasible induction regimen prior to auto‐HCT in frontline MCL: results of SWOG Study S1106

Aggressive induction chemotherapy followed by autologous haematopoietic stem cell transplant (auto‐HCT ) is effective for younger patients with mantle cell lymphoma (MCL ). However, the optimal induction regimen is widely debated. The Southwestern Oncology Group S1106 trial was designed to assess rituximab plus hyperCVAD /MTX /ARAC (hyperfractionated cyclophosphamide, vincristine, doxorubicin and dexamethasone, alternating with high dose cytarabine and methotrexate) (RH ) versus rituximab plus bendamustine (RB ) in a randomized phase II trial to select a pre‐transplant induction regimen for future development. Patients had previously untreated stage III , IV , or bulky stage II MCL and received either 4 cycles of RH or 6 cycles of RB , followed by auto‐HCT . Fifty‐three of a planned 160 patients were accrued; an unacceptably high mobilization failure rate (29%) on the RH arm prompted premature study closure. The estimated 2‐year progression‐free survival (PFS ) was 81% vs. 82% and overall survival (OS ) was 87% vs. 88% for RB and RH , respectively. RH is not an ideal platform for future multi‐centre transplant trials in MCL . RB achieved a 2‐year PFS of 81% and a 78% MRD negative rate. Premature closure of the study limited the sample size and the precision of PFS estimates and MRD rates. However, RB can achieve a deep remission and could be a platform for future trials in MCL .     

Flow cytometry for CD34 determination in hematopoietic grafts

CD34 is a type I transmembrane protein that is expressed on lympho-hematopoietic progenitor and endothelial cells and has a potential adhesion function. Various monoclonal antibodies, whether characterized by glycosylated epitopes or not, are utilized to recognize different subsets of hematopoietic progenitors. Coexpression of membrane markers involving CD34 is an approach to the definition of those subpopulations of cells previously characterized by using in vitro cultures. Thus, in the autologous transplantation procedure, flow cytometry determination of CD34+ cells in the grafts themselves, especially as concerns cytapheresis products, was enhanced with CFU-GM enumeration. This methodology required a standardized protocol with regard to the choice of the monoclonal antibody, had to be to data acquisition and to computer analysis. Within the framework of a multicentric trial, various strategies had to be evaluated. Special attention was paid to obtaining a sensitivity level of 0.1 %. New, standardized approaches are currently in the planning stage, in particular with a view to determining absolute count on the basis of readings generated by the flow-cytometer.     

Tetralogy of Fallot Cardiac Function Evaluation and Intelligent Diagnosis Based on Dual‐Source Computed Tomography Cardiac Images

Tetralogy of Fallot (TOF) is the most common complex congenital heart disease (CHD) of the cyanotic type. Studies on ventricular functions have received an increasing amount of attention as the development of diagnosis and treatment technology for CHD continues to advance. Reasonable options for imaging examination and accurate assessment of preoperative and postoperative left ventricular functions of TOF patients are important in improving the cure rate of TOF radical operation, therapeutic evaluation, and judgment prognosis. Therefore, with the aid of dual‐source computed tomography (DSCT), cardiac images with high temporal resolution and high definition, we measured the left ventricular time–volume curve using image data and calculating the left ventricular function parameters to conduct the preliminary evaluation on TOF patients. To comprehensively evaluate the cardiac function, the segmental ventricular wall function parameters were measured, and the measurement results were mapped to a bull's eye diagram to realize the standardization of segmental ventricular wall function evaluation. Finally, we introduced a new clustering method based on auto‐regression model parameters and combined this method with Euclidean distance measurements to establish an intelligent diagnosis of TOF. The results of this experiment show that the TOF evaluation and the intelligent diagnostic methods proposed in this article are feasible.     

IgA-mediated autoimmune hemolytic anemia in an infant

Autoimmune Hemolytic anemia (AIHA) a relatively uncommon form of hemolytic anemia in children, occurs due to the premature destruction of red blood cells caused by presence of autoantibodies directed against antigens on RBCs. Warm reactive AIHA is the most common form due to IgG isotype of immunoglobulin class binding to autologous RBCs at 37⁰C and confirmed with a positive DAT screening. We present a case of DAT-negative primary warm AIHA in an infant due to IgA antibody. A 10 month old male infant presented with dark colored urine and irritability for past two months, with associated history of fever, diarrhea and vomiting. He had received one red cell transfusion 10 days prior. On physical examination he had pallor with tachycardia without splenomegaly. On investigation his hemoglobin was 5.8 g/dl, WBC 25.9 × 10³/mm³ and normal platelets counts. Peripheral blood smear had spherocytes and biochemical values showed high bilirubin and LDH. Immunohematological work up revelaed polyspecific DAT was negative but monospecific DAT screening showed strong (4+) positivity for IgA and a weak IgG positivity. The patient was diagnosed as IgA-mediated Warm AIHA and was started on prednisolone at 2 mg/kg/day following which hemoglobin improved over the next 2 months. After 2 weeks, prednisolone was tapered and stopped by the end of 3 months. Patients with clinical and laboratory evidence of acute hemolysis, an additional screening for IgA antibody may be done even in cases where poly-specific DAT is negative. Early detection helps in avoiding further investigations and provide efficient management.     

自体腘绳肌腱与同种异体肌腱移植重建前交叉韧带的临床疗效分析

目的探讨关节镜下同种异体胫前肌腱与自体腘绳肌腱重建前交叉韧带（ACL）的临床疗效.方法选择2008年12月至2010年8月昆明医学院第一附属医院骨科膝关节前交叉韧带重建患者46例,其中男27例,女19例,分为A组：自体肌腱移植重建25例,B组：同种异体肌腱移植重建21例.术后进行临床体检及前/后抽屉试验、Lachman试验、膝关节应力X线片、髌骨轴位片及MRI等相关检查,Lysholm评分,IKDC评分,AKS评分.术后随访4～24个月,平均12.4个月.结果两组病例术后Lysholm评分,IKDC评分,AKS评分测试结果差异无统计学意义（P〉0.05）.结论同种异体与自体肌腱重建ACL的疗效相近,是重建ACL良好的移植物之一.