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排序方式: 共有300条查询结果,搜索用时 15 毫秒
1.
我国α—淀粉酶(EC3.2.1.1)生产是用枯草杆菌BF—7658菌株,它产生的酶是个复杂酶系。其中混杂的蛋白酶未见酸性蛋白酶。发酵液经热处理后,测蛋白酶活力。结果,碱性蛋白酶耐热性比中性蛋白酶强。发酵液经55℃,30分钟热处理后,碱性蛋白蛋残存活力与室温(20~25℃)下碱性蛋白酶活力相差不显著(P>0.05),而中性蛋白酶则相差显著(P<0.05)。用电泳法观察发酵液蛋白酶电泳谱型,显示两条蛋白酶区带。 相似文献
2.
Ultrastructural,immunohistochemical and biochemical studies on amylase and ACTH producing lung cancer 总被引:1,自引:0,他引:1
Yutaka Yoshida Michio Mori Tomoko Sonoda Fumio Sakauchi Hiroyuki Sugawara Akira Suzuki 《Virchows Archiv : an international journal of pathology》1985,408(2-3):163-172
Summary Tumour tissue from a lung cancer patient who showed elevated serum amylase and adrenocorticotropin (ACTH) was studied ultrastructurally, immunohistochemically and biochemically. Histologically the tumour was a small cell carcinoma. On electron microscopic examination the tumour cells contained large zymogen-like granules within the cytoplasm. Furthermore, cells which possessed many small dense core granules of the endocrine type were also observed. It was of interest that the large zymogen-like granule-containing tumour cells had microvilli at the apical border, connected by desmosomes and forming lumina showing adenocarcinomatous differentiation. Electrophoretic analysis of the serum revealed that the major elevated amylase was of the salivary type with minor components. Immunostaining clearly demonstrated that most of the tumour cells possessed immunoreactive ACTH, whereas salivary amylase was only found in occasional clusters of the tumour cells. The results seem to indicate that the tumour showed both endocrine and exocrine characteristics - an amphicrine carcinoma, expressing amylase and ACTH simultaneously. 相似文献
3.
4.
Josephine O'Doherty Robert J. Stark 《Pflügers Archiv : European journal of physiology》1982,394(1):26-31
Acetylcholine (ACh) released from vagal terminals increases the permeability of the pancreatic acinar membrane to Na+ and Ca2+ ions. In this report, we compare the induced changes in intracellular Na+ and Ca2+ electrode potentials (ENa and ECa) due to ACh-stimulation of acini with those observed during stimulation with the calcium ionophore, A23187, which mimicks the action of ACh on pancreatic secretion. Stimulation with ACh concentrations varying from 10–8 to 10–5 M and with A23187 concentrations of 10–6 and 10–5 M caused parallel increases in cytosolic Ca2+ and Na+ ([Ca]i, [Na]i). The magnitude of the increases in [Ca]i and [Na]i due to A23187-stimulation further indicate that when presented with a calcium challenge the acinar cells continue to regulate [Ca]i close to physiological levels and suggest that the observed increases in ionized calcium could reflect much larger increases in complexed Ca2+. ACh-stimulation following removal of either extracellular Na+ or Ca2+ ions, eliminated the intracellular increases found when the removed ions is present, but did not affect the increases usually found with the other ion. The independence of the permeability changes to either the presence of Ca2+ or Na+ indicates the ACh-induced currents carried by Na+ and Ca2+ are also independent. The selective translocation of Na+ and Ca2+ during acetylcholine-stimulation in a manner analogous to the changes observed when ionophore A23187 was used as stimulus, indicates the ability of the activated acinar membrane to function as an ionophore. 相似文献
5.
尿胰蛋白酶原—2测定对急性胰腺炎的早期诊断价值 总被引:1,自引:0,他引:1
目的 探讨尿胰蛋白酶原-2测定在急腹症中筛选急性胰腺炎的价值。方法 对346例连续就诊的急腹症病人同时测定尿胰蛋白酶原-2及血、尿淀粉酶。结果 41例急性胰腺炎中有38例尿胰蛋白酶原-2阳性,敏感性为92.7%,305例非胰腺炎的急腹症病人有15例假阳性,特异性为95.1%。5例重症急性胰腺炎中尿胰蛋白酶原-2全部阳性。结论 尿胰蛋白酶原-2是急腹症病人筛选急性胰腺炎快而简便的方法,具有较高的特异性和敏感性。 相似文献
6.
目的:观察通腑解毒方联合六合丹辅助西医治疗急性胰腺炎的临床疗效及对患者肠功能屏障、肝功能、炎症反应的影响。方法:将157例急性胰腺炎患者采用简单随机法分为西医组(n=78)和辨证组(n=79)。西医组给予西医治疗,辨证组在西医组治疗基础上给予通腑解毒方灌肠联合六合丹外敷治疗。比较两组临床相关指标、中医症候积分、疗效、28 d病死率、血清学指标。结果:辨证组总有效率高于西医组,腹痛、腹胀缓解时间及首次排便时间、进食时间均短于西医组(均P<0.05)。治疗后,两组各项中医症候积分较治疗前下降,且辨证组低于西医组(均P<0.05)。治疗后,两组血、尿淀粉酶及二胺氧化酶(DAO)、D-乳酸、谷草转氨酶(AST)、C反应蛋白(CRP)、单核细胞趋化因子-1(MCP-1)、谷丙转氨酶(ALT)水平均较治疗前下降,且辨证组低于西医组(均P<0.05)。两组28 d病死率比较,差异无统计学意义(P>0.05)。结论:通腑解毒方联合六合丹辅助西医治疗急性胰腺炎可促进腹痛、腹胀的缓解,保护肠功能屏障,抑制炎症反应,提高疗效。 相似文献
7.
Irma C. W. de Peusner Carlos J. Perec Francisco J. E. Stefano 《Naunyn-Schmiedeberg's archives of pharmacology》1979,308(3):217-221
Summary Amylase and K+ release were measured in slices obtained from innervated and sympathetically denervated rat parotid glands. Amylase release induced by noradrenaline was found to be increased after chronic denervation. The postjunctional component of supersensitivity to noradrenaline was evidenced by an increase in the maximal response to this agonist. The maximal response of denervated glands was blocked by propranolol 10–5 M. On the other hand, chronic denervation did not modify the -adrenoceptor-mediated response, K+ release, either in the presence or in the absence of ouabain. It is concluded that, after sympathetic denervation, postjunctional supersensitivity develops for the -adrenoceptor-mediated but not for the -adrenoceptors-mediated effects of noradrenaline. 相似文献
8.
S. R. Handrakala ulian A. Crasta hameem Shariff 《Indian journal of otolaryngology and head and neck surgery》2003,55(4):275-277
Sialomrgaly of suhmandibultir gland, whether inflammatory or neoplastic can be clinically mistaken for lymphoid metastasis
especially when encountered in an elderly patient. Other than the neoptastic lesions and usual inflammatory lesions rarely
amylase crystalloids can evoke a granulomatous response and can result in sialomegaly. We report an interesting case which
presented as a submandibular swelling and was clinically diagnosed as metastasis where the diagnosis of granulomatous response
to crystalloids was made at FNAC. The aim of this report is to bring an awareness of the existence of such lesions and to
highlight the role of FNAC in diagnosis of such lesions in order to avoid unnecessary surgeries. 相似文献
9.
10.
目的 :建立以α- 2 -氯 - 4-硝基苯 -半乳糖吡喃麦芽糖苷 ( Gal- G2 - CNP)作底物的α-淀粉酶 (α-amylase)的直接测定方法。方法 :利用国外最新合成的 Gal- G2 - CNP( alpha- 2 - chloro- 4- nitrophenyl-galactopyranosylmaltoside)作底物 ,用速率法测定α-淀粉酶活力。结果 :线性范围可达 30 0 0 U/L ( r=0 .9998) ,变异系数为 0 .30 %~ 0 .53%。与宝灵曼公司的 ET- G7- PNP法 〔1〕的相比 :r=0 .9998,Y=0 .76X 0 .2 0 ( n=2 0 0 ) ,与 G3 - CNP的相比 r=0 .9999,Y=1 .0 1 X 1 1 .7( n=2 0 0 )。结论 :简便、直接、灵敏、经济、试剂单一稳定、反应转化率高、线性范围宽、基质本身自然水解极微 ,各项指标符合国际临床化学学会( IFCC)提出的“理想的淀粉酶检测方法”的要求〔2〕。适于各种类型的自动分析 相似文献