动脉粥样硬化性脑梗死患者血脂分析

目的探讨血脂异常血症与动脉粥样硬化性脑梗死的关系。方法指标TCH、TG采用酶法检测，HDL—C、LDL—C采用磷钨酸-镁沉淀法检测。结果患病组与对照组血脂四项检测结果比较差异有显著性（P〈0．01）。结论血脂异常与动脉粥样硬化性脑梗死的发生有密切的关联，是动脉粥样硬化性病变形成的必要因素，注意脂类的合理摄入是预防和治疗动脉粥样硬化性脑梗死的重要方面。     

2型糖尿病合并高血压,冠心病的脂代谢比较

目的：观察２型糖尿病合并高血压、冠心病的脂代谢变化。方法：对２０４例２型糖尿病患者分组比较血脂代谢指标，同时观察新的脂代谢指标总胆固醇与高密度脂蛋白的比值（ＴＣＨ／ＨＤＬ－ｃ）在糖尿病合并高血压、冠心病、不同空腹血糖水平等情况下的变化。结果：ＴＣＨ／ＨＤＬ－ｃ较其它单项的血脂代谢指标更易反映出各组间差异。结论：ＴＣＨ／ＨＤＬ－ｃ有可能作为２型糖尿病患者反映脂代谢紊乱的一个有效指标，与糖尿病合并大血管病变有一定关系。     

Green tea extract reduces blood pressure,inflammatory biomarkers,and oxidative stress and improves parameters associated with insulin resistance in obese,hypertensive patients

Green tea (GT) consumption is known to be associated with enhanced cardiovascular and metabolic health. The purpose of this study is to examine the hypothesis that supplementation with GT alters insulin resistance and associated cardiovascular risk factors in obese, hypertensive patients. In a double-blind, placebo-controlled trial, 56 obese, hypertensive subjects were randomized to receive a daily supplement of 1 capsule that contained either 379 mg of GT extract (GTE) or a matching placebo, for 3 months. At baseline and after 3 months of treatment, the anthropometric parameters, blood pressure, plasma lipid levels, glucose levels, creatinine levels, tumor necrosis factor α levels, C-reactive protein levels, total antioxidant status, and insulin levels were assessed. Insulin resistance was evaluated according to the homeostasis model assessment–insulin resistance protocol. After 3 months of supplementation, both systolic and diastolic blood pressures had significantly decreased in the GTE group as compared with the placebo group (P < .01). Considerable (P < .01) reductions in fasting serum glucose and insulin levels and insulin resistance were observed in the GTE group when compared with the placebo group. Serum tumor necrosis factor α and C-reactive protein were significantly lower, whereas total antioxidant status increased in the GTE group compared with the placebo (P < .05). Supplementation also contributed to significant (P < .05) decreases in the total and low-density lipoprotein cholesterol and triglycerides, but an increase in high-density lipoprotein cholesterol. In conclusion, daily supplementation with 379 mg of GTE favorably influences blood pressure, insulin resistance, inflammation and oxidative stress, and lipid profile in patients with obesity-related hypertension.     

基本药物制度下乡镇卫生院面临的挑战及建议

随着基本药物制度在基层医疗机构推广,不仅其本身存在的问题开始凸现,而且对乡镇卫生院的挑战也日益的明显。从基本药物制度存在的问题出发,分析其对乡镇卫生院的挑战,进而提出应对建议。     

Triplet repeat polymorphism within the NOTCH4 gene located near the junction of the HLA class II and class III regions in narcolepsy

A polymorphic (CTG)_n, microsatellite repeat was found in the signal peptide domain of the NOTCH4 gene located near the junction of the class II and class III regions of the human major histocompatibility complex. This gene belongs to a multigenc family of NOTCH originally identified as a differential factor of neuronal cells. To ascertain whether the NOTCH4 gene is involved in the development of neurogenic disease, narcolepsy, which is known to be tightly associated with HLA-DR15, this microsatellite polymorphism of the (CTG)_n repeat was analyzed in Japanese patients with narcolepsy. One allele, 9 repetitions of CTG (Leu) was significantly increased in the patient group. However, the significant increase of this allele in the patient group could be explained by a strong linkage disequilibrium with the HLA class II alleles, DRB1*1501, DQA1*0102 and DQB1*0602, which were more strongly associated with the disease. These results suggest that the (CTG)_n repeat polymorphism in NOTCH4 does not primarily determine the susceptibility to narcolepsy.     

TCH方案术前新辅助化疗对HER-2阳性乳腺癌的疗效观察

目的 观察TCH方案术前新辅助化疗对HER-2阳性乳腺癌的近期疗效及毒副反应.方法 37例女性HER-2阳性乳腺癌患者给予TCH方案术前新辅助化疗,治疗3～4周期后进行疗效和毒副反应评价.结果 37例患者中CR 5例,PR 28例,NC 4例,PD 0例,有效率为89.2％.主要毒副反应:中性粒细胞减少22例(59.5％),恶心呕吐15例(40.5％),肝功能异常8例(21.6％).结论 TCH方案用于HER-2阳性乳腺癌患者术前辅助化疗疗效较好,毒副反应可耐受.     

中药综合措施对肾功能衰竭大鼠生化指标的影响

目的:通过对治疗前后5/6肾切除后肾功能不全模型大鼠各项生化指标的检测,进一步验证中药综合措施对肾功能不全的治疗疗效。方法:采用5/6肾切除肾衰模型,一次性摘除右肾并切除左肾的2/3,随机分为病理组、通脉组、结肠组、综合组及正常对照组,治疗4周后,采血检测Bun、Scr及血脂四项TCH、TG、HDL-C、LDL-C,进行组间疗效比较。结果显示:中药综合措施可改善模型大鼠的一般状态,降低血清BUN、Scr,调节脂质代谢。结论:中药综合措施可有效地延缓肾功能不全大鼠的恶化进程,优于单一药物组及西药对照组。     

Transient neonatal hypothyroidism is associated with elevated serum anti-thyroglobulin antibody levels in newborns and their mothers

Transient congenital hypothyroidism (TCH) was detected in 6 of 35,067 newborns (1:5845 births) screened in Iran. Antithyroglobulin antibodies positivity was present in 4 of 6 (66.7%) of those with TCH and in 6 of 106 (5.7%) of those with "transient hyperthyrotropinemia and normal" diagnoses (P = .0005), but positivity was similar in newborns with transient hyperthyrotropinemia versus normal neonates (P = .397).     

分枝杆菌菌种快速荧光初步鉴定方法的研究

目的　使用液体培养基,建立一种分枝杆菌(Mycobacterium)菌种初步鉴定的非放射快速荧光检测法。方法 应用自制的非放射荧光检测管,利用水溶性对硝基苯甲酸(PNitro benzoic Acid,PNB)和噻吩-2-羧酸肼(2-Thiophene carborylic acid hydrazide,TCH)对15株分枝杆菌标准菌株和78株分枝杆菌临床分离菌株做体外抑菌试验,确定其在菌群鉴定试验中的应用浓度。结果 PNB的应用浓度为100μg/ml,可有效地区别结核杆菌复合群与非结核分枝杆菌;TCH的应用浓度为2.5μg/ml,可有效地区别结核分枝杆菌和牛分枝杆菌。菌群鉴定结果7～10d可得到报告。结论 本法经济实用,并将分枝杆菌菌群鉴定结果报告时间由传统的LJ方法的28d缩短为7～10d。     

Concentrations of Δ9-tetrahydrocannabinol,cocaine and 6-monoacetylmorphine in hair of drug abusers

Hair samples taken from 850 individuals with presumed drug abuse were tested simultaneously forΔ⁹tetrahydrocannabinol (THC), cocaine, heroin, the primary heroin metabolite 6-monoacetylmorphine (6-MAM) and morphine. The drugs were extracted with methanol under sonication. Compared to other extraction procedures this solvent extraction technique provides high extraction yields and less experimental effort. The analyses were carried out using gas chromatography - mass spectrometry (GCMS) in selected ion monitoring (SIM) mode. This procedure allows the simultaneous detection of amphetamine, methylenedioxyamphetamine (MDA), methylenedioxymetbamphetamine (MDMA) and methylenedioxyethylamphetamine (MDE). THC was found in 104 (12.2%), cocaine in 230 (27%) and 6-MAM in 141 (16.6%) samples. In addition to 6-MAM, morphine was detected in 87 (10.2%) and heroin in 38 samples (4.5%). The concentrations found were in a range 0.009-16.7 ng/mg for THC, 0.037-129.68 ng/mg for cocaine, 0.028-79.82 ng/mg for 6-MAM, 0.045-53.14 ng/mg for heroin and 0.011-7.800 ng/mg for morphine. The statistical distribution of the drug concentrations compared with the self-reported consumption behaviour of the users may possibly lead to a better understanding of the relationship between drug dosage and corresponding concentrations in hair.