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排序方式: 共有343条查询结果,搜索用时 31 毫秒
1.
本文介绍免疫组化法检测小儿白血病瘤细胞(P-gp)。检测30例小儿白血病细胞膜P-gp,观察其原发耐药性。30例中有8例p-gp阳性,总阳性率26.67%,其中ANLL单项阳性率33.33%,ALL为20%。本组病例耐约性最高的是CML,其次是ANLL,ALL组最低。同时发现MDR阳性者复发率高,MDR阴性者复发率低,多因素分析表明MDER对初诊白血病患者预后及抗癌药物选择有重要意义。目的临床评价MDR采用检测mdr1mRNA或F-gp,本文认为采用简便、快速的免疫组化法检测P-gp在临床上具有较大的实用价值。 相似文献
2.
Rest thallium-201 myocardial perfusion imaging in a patient with leukaemic infiltration of the heart
A. Cahid Civelek Jeffrey A. Brinker Edwaldo E. Camargo Jonathan M. Links Henry N. Wagner Jr. 《European journal of nuclear medicine and molecular imaging》1992,19(4):306-308
Despite the high incidence of leukaemic infiltration of the heart, only 8 cases of atrioventricular block due to leukaemia have been reported in the literature. Improvement in the heart block associated with disappearance of the leukaemic infiltrate has not been reported. A rest thallium-201 study was used in a 65-year-old man to demonstrate leukaemic infiltration of the heart which was associated with complete heart block. After chemotherapy, when the tumour burden was reduced and the leukaemia in remission, his heart block resolved, and a follow-up thallium scan was normal.
Offprint requests to: A.C. Civelek 相似文献
3.
Epidural infiltration by leukemic cells in Acute Non Lymphoblastic Leukemia (ANLL), and extradural granulocytic sarcomas as a cause of spinal cord compression are extremely rare phenomena. Very few cases have been recorded in the literature. In this brief case report we describe a young patient with acute myeloblastic leukemia (M4, FAB) who presented with sudden spinal cord compression during relapse after bone marrow transplantation. The literature on this subject is also reviewed. 相似文献
4.
Cell death by apoptosis in acute leukaemia 总被引:5,自引:0,他引:5
G D Baxter R J Collins B V Harmon S Kumar R L Prentice P J Smith M F Lavin 《The Journal of pathology》1989,158(2):123-129
We have previously demonstrated that when freshly isolated childhood T-cell acute lymphoblastic leukaemia cells are incubated in growth medium after isolation from blood, chromatin is rapidly cleaved into nucleosomal sized fragments that are multiples of 200 bp. The fragmentation is similar to that observed in other types of cells undergoing apoptosis or programmed cell death. In this study we describe a more comprehensive approach to the study of DNA fragmentation in leukaemia. Fragmentation was observed in freshly isolated cells from patients with T-cell acute lymphoblastic leukaemia and in one with common acute lymphoblastic leukaemia. Frozen samples of T-cell acute lymphoblastic leukaemia, common acute lymphoblastic leukaemia, and acute myeloid leukaemia cells also showed fragmentation of DNA. However, no fragmentation was evident in normal leukocytes treated under the same conditions. Ultrastructural studies on the isolated leukaemia cells demonstrate that the chromatin cleavage observed biochemically is associated with morphological changes characteristic of apoptosis. 相似文献
5.
V. Turinelli T. Marchal F. Ponce C. Bonnefont-Rebeix C. Fournel-Fleury 《Comparative clinical pathology》2005,13(3):109-118
Among 276 canine lymphomas referred to the Haematology–Cytology–Immunology laboratory of the Lyon Veterinary School between 1997 and 2003, there were five aggressive large granular lymphocyte (LGL) malignancies. The five dogs were clinically examined and followed up. Cytological and histological analyses of the liver, spleen, lymph nodes, intestine and bone marrow were performed. The immunophenotype and proliferation index were established. The most significant clinical finding was that of an aggressive clinical course, the presence of hepatomegaly and/or splenomegaly, an abdominal lymphadenopathy, anaemia in four cases and blood and bone-marrow involvement in two cases. The cytological presentation was a diffuse infiltration of atypical, large granular lymphoid cells. Two cases were of the null type (CD3–, CD79a–, CD4–, CD8–), and three were of the T-cell type (CD3+, CD79a–, CD4–, CD8+). The proliferation index was high in all cases, with a median of 54.4%. The histological presentation of the null-type cases was an infiltration of the livers portal triads and the spleens red pulp. The T CD8+ cases showed two different patterns, characterised by infiltration: in the first, of all the intestines layers, the livers portal triads, the spleens red pulp and the lymph nodes and, in the second, infiltration of the livers sinusoids, the spleens red pulp. Although these aggressive LGL lymphomas are still poorly known, they may be compared to three types of human lymphoma: aggressive NK cell lymphoma/leukemia, hepatosplenic T-cell lymphoma and enteropathy-type T-cell lymphoma. 相似文献
6.
用可移植性小鼠淋巴细胞性白血病腹水瘤细胞克隆株(LAC-1)细胞免疫同系小鼠,常规方法融合、筛选获得四株分泌特异抗体杂交瘤株。所得McAb能特异地与LAC—1细胞结合,但不能与正常或经ConA转化的小鼠胸腺细胞以及其它多种小鼠瘤细胞起结合反应。ELISA检测显示,McAb与LAC—1细胞培养上清液中提取的病毒作用时未发现阳性反应。细胞介导的细胞毒实验表明,将McAb预先与LAC—1细胞孵育后能显著抑制T杀伤细胞对靶细胞的特异性杀伤作用,免疫印迹试验分析发现McAb识别的LAC—1细胞靶抗原分子量为41KD。 相似文献
7.
The aim of this study was to assess the physical performance in long-term survivors of acute leukaemia in childhood and to
evaluate the effects of anthracycline therapy. Electrocardiography, echocardiography and spiroergometry were carried out on
56 patients aged 9–28 years, of whom 44 patients had been treated with 15–483 mg/m2 doxorubicin (or equivalent). Acute leukaemia had been diagnosed 1.5–16 years earlier. Of the patients 75% reached normal
maximal oxygen uptake, 69% normal oxygen uptake at the anaerobic threshold and 95% normal maximal work rate. Of the patients
75% achieved adequate values for maximal heart rate and 78% normal blood lactate concentration. No difference was seen between
patients treated with and without anthracyclines.
Conclusion The results of this study provide little evidence for cardiopulmonary impairment in long-term survivors of ALL. Both the
cardiac function, as evaluated by ECG and echocardiography, and the physical performance in spiroergometry are normal in a
large number of these patients. Anthracycline treatment does not appear to have a negative effect on these parameters.
Received: 30 October 1996 / Accepted in revised form: 7 October 1997 相似文献
8.
9.
Investigations of excess environmental risks around putative sources: statistical problems and a proposed test 总被引:14,自引:0,他引:14
R A Stone 《Statistics in medicine》1988,7(6):649-660
Individual reactions to a report which identifies an excess of risk near a putative source are determined mainly by some quoted significance level. One reaction, involving a commonly used 'coincidence' argument is given a simple Bayesian explanation. It is argued that interpretations of such reports should if possible allow both for data selection and for uncertainty in the null expectations underlying the significance levels. Tests are proposed, based on the first isotonic regression estimator under an order restriction, which allow for the effects of selecting a study region in the light of the data and have a simple form. Data on cancer incidence around two nuclear plants are used to illustrate. 相似文献
10.
目的:探讨白血病抑制因子(LIF)基因在小鼠动情周期子宫内膜的表达情况。方法:采用RT-PRC技术对小鼠动情周期各个时期LIF基因表达进行检测。结果:动情周期、动情前期和动情期均未检测到LIF基因表达,而动情后期子宫内膜检测到LIF基因强表达。结论:推测LIF基因表达受母体自身激素水平改变的调控,且主要涉及孕激素,而与雌激素关系不大。 相似文献