New frontiers in applied veterinary point‐of‐capture diagnostics: Toward early detection and control of zoonotic influenza

Among the chief limitations in achieving early detection and control of animal‐origin influenza of pandemic potential in high‐risk livestock populations is the existing lag time between sample collection and diagnostic result. Advances in molecular diagnostics are permitting deployment of affordable, rapid, highly sensitive, and specific point‐of‐capture assays, providing opportunities for targeted surveillance driving containment strategies with potentially compelling returns on investment. Interrupting disease transmission at source holds promise of disrupting cycles of animal‐origin influenza incursion to endemicity and limiting impact on animal production, food security, and public health. Adoption of new point‐of‐capture diagnostics should be undertaken in the context of promoting robust veterinary services systems and parallel support for operationalizing pre‐authorized plans and communication strategies that will ensure that the full potential of these new platforms is realized.     

环境水样中痕量铜的浊点萃取-火焰原子吸收光谱测定法

目的建立环境水样中痕量铜的浊点萃取(cloud point extraction,CPE)-火焰原子吸收光谱(flame atomic absorption spectrometry,FAAS)测定法。方法样品在p H 9.5的条件下,加入0.4 ml的1 mmol/L 2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)溶液,0.1%氯化钙溶液0.1 ml,5%(W/V)Triton X-114溶液0.8 ml,40℃加热15 min后离心,采用火焰原子吸收光谱法进行检测。结果在2~240μg/L的线性范围内,所得回归方程为A=0.002 7c+0.024 6,r=0.995 8。以3倍信噪比计算,方法的检出限为0.62μg/L,富集倍数为36.58倍,平均加标回收率为96.28%~98.08%,RSD为1.67%~3.13%。结论该方法简单、灵敏,具有良好的重现性,适用于环境水样中痕量铜的测定。     

Recurrent herpes labialis in the pediatric population: Prevalence,therapeutic studies,and associated complications

Recurrent herpes labialis (RHL) is an incredibly common condition, though the medical literature evaluating pediatric aspects is limited. This paper assesses prevalence and therapeutic studies of pediatric RHL as well as disease complications. A comprehensive literature search of English‐language citations based on PubMed queries of selected terms was performed, with exclusion if methodology was not discussed, or if studies had 10 or fewer patients. RHL prevalence in pediatrics has been assessed by measures of point and periodic prevalence, though methodologic limitations may under‐ or over‐estimate the true prevalence of RHL. Studies have been conducted to evaluate therapeutic safety, tolerability, and efficacy of antivirals in the pediatric population. Pediatric RHL point prevalence ranges from 0.72% to 5.2% depending on the population study and the methodologies used. Pediatric RHL carries a significant public health burden and is often implicated in patients with eczema herpeticum, erythema multiforme, reactive infectious mucositis eruptions, and hypersensitivity reactions. There are few studies that evaluate the rates of occurrence of these sequelae associated with pediatric RHL.     

亚麻籽胶的胶凝性质

采用流变学法测定了亚麻籽胶溶液的胶凝点、熔化点，并采用质构仪、扫描电镜和原子力显微镜等手段研究了影响亚麻籽胶凝胶强度的因素，结果表明亚麻籽胶具有胶凝性，它能形成一种热可逆的冷致凝胶，亚麻籽胶溶液的胶凝点低于其凝胶的熔化点，且亚麻籽胶溶液的胶凝点及其凝胶的熔化点均随冷却的起始温度的升高而升高。亚麻籽胶浓度、溶解温度、pH、NaCl、CaCl2及复合磷酸盐能影响亚麻籽胶的凝胶强度，亚麻籽胶的凝胶强度随着浓度的增加及溶解温度的升高而增强；在pH6～9的范围内，亚麻籽胶的凝胶强度达到最大；NaCl和复合磷酸盐可以降低亚麻籽胶的凝胶强度，低浓度（〈0．3％）的CaCl2可以增强亚麻籽胶的凝胶强度，而高质量分数（〉0．3％）的CaCl2能降低亚麻籽胶的凝胶强度。     

后路椎体间融合术后融合器脱出的原因及其翻修术

目的：探讨后路椎体间融合术后融合器脱出的原因及其翻修方法。方法：10例后路椎体间融合术后融合器脱出行翻修术的病例，其中腰椎滑脱症5例，腰椎管狭窄症3例，腰椎间盘突出症2例。手术节段为L4／54例，L5／S1 3例，L4／5＋L5／S1 3例；融合器应用BAK（旋入型）7例，聚醚醚酮矩形融合器（PEEK）2例，钛金属矩形融合器1例。于首次术后1～90d内行翻修术。就其脱出原因，再次手术方法及疗效进行分析。结果：融合器脱出的原因：矩形融合器中2例型号太小，1例放置位置偏后；旋入型融合器1例定位错误，其余大都因两枚融合器之间发生碰撞挤压所致。翻修术后获得完整随访资料者8例，随访时间10-36个月，平均17个月，均得到融合，无融合器再次移位或脱出以及内同定的断裂，1例伴有足下垂者遗留患足背伸肌力减弱，其余病例神经症状消除。结论：后路腰椎椎体间融合术后融合器脱出的可能原因有融合器型号偏小导致椎间隙撑开不够，放置位置偏后，两枚融合器不对称或距离太近发生碰撞等。早期、积极的手术翻修能取得较好的疗效。     

等电聚焦电泳分析红细胞Gd(-)壮族富宁型〔Gd(-)Zhuang-Funing〕

本文采用聚丙烯酰胺凝胶等电聚焦电泳(IEF),借助园盘电泳仪的简易装置,对正常 B 型G6PD 及变异型 G6PD 进行分析,获得了满意效果。     

按压骨穴调节围绝经期妇女心脏植物神经功能的研究

目的观察按压骨穴对围绝经期妇女心脏植物神经功能的影响。方法设按压骨穴组30例、空白对照组30例。用美国BraemarDL700型动态心电检测仪,检测试验前后的心率变异性(HRV):TF代表总频,LF代表交感神经活性,HF代表迷走神经活性,LF/HF代表交感神经与迷走神经均衡性。结果按压骨穴后4项指标均有显著下降(P<0.01,P<0.05)。空白对照组变化不显著(P>0.05)。骨穴组4项指标的变化率与对照组比较均有显著性差异(P<0.01)。结论按压骨穴有抑制心脏交感神经活性的良性调整作用,此效应具有相对的特异性。     

轴突分叉点位置计算法适用性的分析

某些中枢神经元通过分叉轴突向两个以上核团投射。轴突分叉点的位置通常由计算轴突主干传导时间予以估测。但是,本工作用该法的4个公式计算细胞内或细胞外记录到的数据未能得到一致和可信的数值。结果提示,计算法的原理是不合理的,并至少对在细胞内或细胞外记录条件下估测轴突分叉点是不适用的。