HPLC法测定鹿蹄草中槲皮素的含量

目的建立HPLC法测定鹿蹄草中槲皮素含量的方法。方法采用HPLC法,色谱柱：hgilent Eclipse XDB—C18柱（4．6mm×150mm,5μm）;流动相：甲醇-0．1％磷酸（50：50）;检测波长：370nm。结果槲皮素在0．092～0．46Pg范围内线性关系良好,平均回收率为99．24％,RSD=0．70％。结论方法简便、准确、灵敏度高、重复性好,能有效测定鹿蹄草药材中槲皮素含量。     

S-T segment elevation during exercise: Electrocardiographic and arteriographic correlation in 38 patients

The clinical significance and underlying mechanisms of S-T segment elevation during exercise were evaluated by correlating the exercise-induced S-T elevation with the coronary arteriograms and left ventriculogram in 38 patients. Of these, 37 (97 percent) showed significant coronary artery disease; 71 percent of these had proximal lesions. Of 27 patients with old myocardial infarction manifested in the electrocardiogram at rest, 25 had significant coronary artery disease and a ventricular aneurysm. All 11 patients with no previous myocardial infarction in the electrocardiogram at rest had significant coronary artery disease but only 2 (18 percent) had a ventricular aneurysm. One patient had a ventricular aneurysm without coronary artery disease. The sites of S-T elevation correctly localized the area of ventricular aneurysm of 30 (91 percent) of 33 instances and the area of the compatible diseased vessels in 38 (95 percent) of 40 instances.Our data suggest that (1) S-T elevation during exercise in the absence of a pattern of previous myocardial infarction in the electrocardiogram at rest indicates significant proximal coronary artery disease without ventricular aneurysm, whereas in the presence of such a pattern it is indicative of both ventricular aneurysm and significant proximal coronary artery disease; (2) the sites of S-T elevation accurately identify the location of ventricular aneurysm and the compatible diseased vessels; and (3) ischemia and abnormal wall motion may independently or additively underlie the mechanism for S-T elevation during exercise.     

Folin比色法测定鹿衔草提取物中总多酚的含量

目的：建立Folin比色法测定鹿衔草提取物中总多酚的含量。方法：采用分光光度法测定。以没食子酸为指标,检测波长730nn]。结果：没食子酸在1．08μg／mL～6．48μg／mL范围内线性良好,相关系数为0．9999,加样回收率99．7％,RSD为1．2％（n=9）。结论：本法测定鹿衔草提取物中总多酚的含量,结果准确可行。     

Functional and esthetic restorative treatment with preheated resins in a patient with ectodermic dysplasia: A clinical report

Ectodermal dysplasia is a heterogeneous genetic condition affecting 1.6 to 22 per 100 000 people. Oral manifestations associated with this condition include hyperdontia, hypodontia, microdontia, and conical teeth. Traditional treatment consists of a combination of orthodontic and rehabilitation therapies. The initial treatment stage uses removable prostheses and interim crowns for long periods, thus increasing risks for developing secondary caries. This clinical report describes the use of direct composite resin bonding with preheated compactable resins applied to vacuum-formed trays filled with clear silicone. This restorative treatment provides predictable, inexpensive, minimally invasive, functional, and esthetic recovery before orthodontic treatment.     

Fenestrated endovascular aortic aneurysm repair using physician-modified endovascular grafts versus company-manufactured devices

Objective

Fenestrated endografts are customized, patient-specific endovascular devices with potential to reduce morbidity and mortality of complex aortic aneurysm repair. With approval from the U.S. Food and Drug Administration, our center began performing fenestrated endovascular aneurysm repair through a physician-sponsored investigational device exemption (IDE #G130210), using both physician-modified endografts (PMEGs) and company-manufactured devices (CMDs). Because these techniques are associated with specific advantages and disadvantages, we sought to investigate differences in outcomes between PMEG and CMD cases.

Effects of aminoglutethimide, metyrapone, and ascorbic acid on testicular metabolism of cholesterol

The effects of aminoglutethimide (AG), metyrapone (MET), and ascorbic acid (AA) on the conversion of labeled cholesterol to testosterone (T) by the mature rat testis were examined and compared under identical in vitro conditions. In cell-free testicular homogenates, formation of labeled T from cholesterol-4-¹⁴C was depressed in a dose-related manner by AG and MET in concentrations ranging from 0.03 to 1.0 mM; AG was the more potent inhibitor at each concentration tested. Within this range of concentrations, AA induced a slight stimulation of the conversion of cholesterol-4-¹⁴C to T, with inhibition demonstrable only above 5.0 mM. Halfmaximal inhibition of T formation was attained with 0.1, 0.3, and 6.4 mM concentrations of AG, MET, and AA, respectively. When the synthesis of T was almost completely prevented by any of these agents, no accumulation of known intermediates between cholesterol and T could be demonstrated. Under these conditions, MET had no effect on the conversion of pregnenolone-4-¹⁴C to T or several T precursors or on the rate of metabolic degradation of testosterone-4-¹⁴C. Since the generation of the six-carbon fragment resulting from side chain cleavage of cholesterol-26-¹⁴C by rat testicular mitochondrial suspensions was also inhibited by AG, MET, and AA, the depression of steroid production affected by these agents in the testis appears to be related to their capacity to block the reaction cholesterol pregnenolone, as previously demonstrated in the adrenal and ovary. AG, MET, and AA thus inhibit cholesterol cleavage in adrenal and gonadal tissues as well as 11β-hydroxylation in the adrenal. In view of the similarities between the enzyme systems affected, it is likely that AG, MET, and AA exert their inhibitory effects on steroid-forming tissues through similar mechanisms.     

Influenza Vaccination Documentation Rates During the First Year After Diagnosis of Diffuse Large B Cell Lymphoma

IntroductionInfluenza infection causes significant morbidity and mortality in patients with cancer, and annual influenza vaccination for individuals with cancer is recommended. We sought to examine the documentation rate of influenza vaccine administration, refusal, or counseling in the first year after diagnosis of diffuse large B cell lymphoma (DLBCL) for patients across 3 hospitals in 2 health care systems.Patients and MethodsDocumentation of vaccine administration, refusal, or counseling by physicians, advanced practice providers, or nursing staff during the first period of influenza vaccine availability after diagnosis (August to April) was assessed in medical records of patients diagnosed with DLBCL between February 2015 and October 2017 who presented to Emory St. Joseph Hospital (community hospital), Winship Cancer Institute of Emory University (academic medical center), or Grady Memorial Hospital (county hospital).ResultsOf the 57% (61/107) of newly diagnosed patients with DLBCL who had vaccine-related documentation, 43% refused vaccination. Counseling was not documented for any patient. Inpatient nursing performed 75% of all documentation. Primary oncologists documented vaccination in 4% of all cases.ConclusionDespite the limited immunization documentation and high refusal rates observed in this study, the influenza vaccine refusal rate was lower than the average for the United States, the state of Georgia, and the previous studies of patients with cancer. Although routine outpatient vaccination occurs, improvements in screening, strategies for sharing patient vaccine-related information, and counseling of patients who refuse the vaccine are needed. Further work is also needed to determine the effectiveness of influenza vaccination in patients receiving anti-cancer therapy.     

Trends in use of the only Food and Drug Administration-approved commercially available fenestrated endovascular aneurysm repair device in the United States

Background

Fenestrated endografts are customized, patient-specific, endovascular devices with potential to significantly reduce morbidity and mortality of short-neck infrarenal and juxtarenal abdominal aortic aneurysm repair. The Zenith fenestrated endovascular graft (ZFEN) for abdominal aortic aneurysms (Cook Medical, Bloomington, Ind), Food and Drug Administration-approved in 2012, remains the only fenestrated device available in the United States. This technology is among the most technically complex catheter-based procedures and, therefore, inherently associated with serious risk for device-related complications. We sought to define patterns of physician and hospital adoption of ZFEN.