Spindle-cell glioblastoma or gliosarcoma?

'Gliosarcomas' have long been considered to be mixed gliomas and sarcomas. The present study failed to define criteria which clearly delineate 'gliosarcomas' from glioblastoma multiforme and suggests that 'gliosarcomas' should be considered as spindle cell glioblastomas. A total of six cases originally diagnosed as 'gliosarcomas' were compared with four cases of glioblastoma multiforme. No clinical or prognostic features were defined which would clearly separate 'gliosarcomas' from glioblastoma multiforme. Macroscopically, biopsies from 'gliosarcomas' ranged from firm, apparently well-circumscribed tumours to poorly circumscribed lesions with a soft consistency resembling glioblastoma multiforme. Histology revealed a continuous spectrum in which 'gliosarcomas' with large reticulin-rich areas of spindle cells merged with typical glioblastomas containing only small islands of spindle cells and reticulin staining. Immunocytochemistry for glial fibrillary acidic protein (GFAP); S100 protein and alpha-smooth muscle actin (ASMA) showed that the majority of cells in reticulin-poor areas of 'gliosarcoma' and glioblastomas expressed S100 protein and GFAP; many expressed ASMA and some expressed both GFAP and ASMA. Spindle cells in reticulin-rich areas of 'gliosarcomas' and glioblastomas most frequently expressed ASMA but many cells also expressed S100 protein and GFAP; some cells expressed both GFAP and ASMA. The results of this study and a review of the literature suggests that there is a clinical, radiological and pathological continuum with glioblastoma and 'gliosarcoma' at different ends of the spectrum. It is suggested, therefore, that most, if not all, 'gliosarcomas' be redesignated as spindle cell glioblastomas and not be considered as a mixture of glioma and sarcoma.     

一种西门子新型X线机的综合性能与安装

本文叙述西门子型号为Polydoros 80/100的新型X线主机,该主机采用微电脑控制技术、光纤维传输技术及中频技术,X射线剂量精度高,影像质量优良,故障率低,是一种技术较为成熟的X线主机,也是当今西门子公司产品的主流机种。该机的安装调试具有一定难度,本文同时针对这一问题进行讨论,并总结归纳出一套安装调试全过程。     

Anti-S-100 Serum blocks long-term potentiation in the hippocampal slice

S-100 is a calcium-binding, glial protein which has been shown to be involved in behavioral learning and memory tasks. Long-term potentiation (LTP) in the hippocampus is a long-lasting enhancement of synaptic efficacy evoked by repetitive afferent stimulation. When anti-S-100 serum is applied by pressure ejection onto the stratum radiatum of area CA1 of the hippocampal slice, the amplitude of the extracellularly recorded population spike is not affected. However, repetitive stimulation of the afferents during S-100 application failed to produce LTP. At a distant site in the same slice, LTP occurs normally. Preimmune normal rabbit serum had no effect on the development of LTP. It appears that S-100 protein is involved in the establishment of LTP.     

口腔黏膜下纤维性变S100蛋白表达

目的 :研究口腔黏膜下纤维性变中S10 0蛋白的表达水平及其与正常口腔黏膜上皮、异常增生上皮的比较。方法 :应用免疫组织化学 (SP)法 ,检测 10例口腔黏膜下纤维性变、10例上皮异常增生、8例正常口腔黏膜上皮S10 0蛋白表达水平 ,并作对比研究。结果 :S10 0蛋白在正常口腔黏膜上皮中无表达 ,口腔黏膜下纤维性变上皮中和异常增生上皮中S10 0的阳性表达率为 5 0 %,后两者的阳性表达率明显高于正常黏膜组上皮 (P <0 .0 5 ) ;口腔黏膜下纤维性变组与异常增生上皮组比较 ,差异无显著性 (P >0 .0 5 )。结论 :口腔黏膜下纤维性变发生发展与S10 0蛋白过表达密切相关 ,S10 0蛋白表达强度与异常增生上皮表达强度一致。     

颈前部皮神经构筑研究

目的　为研究皮肤疾病和损伤提供皮神经构筑资料。方法　用S 10 0蛋白免疫组化染色法研究正常成人颈前部胸锁乳突肌前、后缘皮肤各层内皮神经的数量、分布及构筑特点。结果　 ( 1)皮肤乳头层内皮神经的小分支多分布在皮突下和乳头内血管丛附近。 ( 2 )真皮网状层内神经多位于小血管附近和汗腺、皮脂腺以及毛囊周围 ,神经分支呈串珠状或波纹形。 ( 3 )皮下组织内神经干和神经网同时存在。结论　皮肤内感觉神经树的分布及构筑特点与血管树相似。     

UF-100全自动尿液流式细胞分析仪影响因素的探讨

UF-100全自动尿液分析仪是日本Sysm ex公司20世纪90年代推出的结合流式细胞学技术(FCM)与电阻抗(electric im pedance,IM P)及荧光染料染色(fluorescencesta in ing),对尿液成分进行鉴别及计数,可以快速筛选、及时报告,提供红细胞(RBC)、白细胞(W BC)、管型(CA ST)、上皮细胞(E     

Triton X-100存在下分光光度法快速测定尿锌

尿样中加入Triton X-100后,在尿蛋白和其它物质存在下可用可见分光光度法直接而快速地测定尿锌.该法比目前其它方法更简便,尤其适合于临床快速分析.经40例正常人尿锌含量分析,结果((?)±s)为0.789±0.256μg/ml,与文献值基本一致.     

大鼠全脑照射后早期血清S-100B变化特征

目的 观察大鼠全脑不同剂量照射后1月内不同时间点血清中S-100B的变化,以探讨S-100OB在早期放射性脑损伤中的变化特征.方法 对大鼠予4 MeV电子线分别单次全脑照射2、10和30 Gv制备大鼠早期放射性脑损伤模型.采用酶联免疫吸附试验(ELISA)检测照射后1 h、6 h、12 h、24 h、3 d、1周、1个月血清中S-100B的浓度.结果 大鼠血清S-100B浓度在照射后6 h升高最明显,照射后12 h浓度下降,照后24 h再升高(除外2 Gy组为在照射后3 dR升高),随后下降直到最后观察时间点.结论 大鼠全脑照射后血清S-100B水平发生了变化,不同剂量组血清S-100B浓度有显著性差异,剂最越高,浓度越高.结果 提示S-100B可能会成为检测早期放射性脑损伤的重要血清指标.