“美容牌”染发剂对雄性小鼠生殖细胞非程序DNA合成的诱导

本文应用雄性小鼠生殖细胞非程序DNA合成(UDS)检测方法研究了“美容牌”染发剂对雄性生殖细胞DNA的损伤作用。发现该染发剂诱发的UDS与药物剂量呈线性正相关。提示此类染发剂对雄性生殖细胞DNA有损伤作用。其遗传毒理效应应引起注意。     

Setting out the frame conditions for feasible use of FFPE derived RNA

Introduction

The usage of formalin-fixed paraffin embedded (FFPE) tissue is characterized by its long shelf-life and simple handling. Therefore it is the most commonly available tissue specimen in routine diagnostics and histological studies. Formaldehyde fixation may result in RNA degradation and cross linking with proteins, while storage conditions also affect RNA integrity. The present study was designed to investigate the influence of these factors on RNA analysis.

Malignant tumor of humerus with features of "adamantinoma" and Ewing's sarcoma

This female patient was born in 1935 and died in 1976 with a malignant bone tumor involving the proximal humerus following multiple local recurrences, axillary lymph node metastases, and pulmonary metastases. Histologically, over the course of time, there was an increase in features of an atypical Ewing's sarcoma, at the expense of findings of a typical, extra-cranial so-called adamantinoma. In contrast to another report, our case showed neither epithelial nor endothelial features on fine structural examination, but rather primitive mesenchymal cells, as is described for Ewing's sarcomas.     

Separation distress and attachment in surrogate-reared squirrel monkeys.

Surrogate-reared infant squirrel monkeys were exposed to various conditions of separation from their surrogate. Infants showed significant increases in plasma levels of cortisol when they were placed in an unfamiliar environment during the separation period. Changes in behavior, but not cortisol, were observed under conditions in which the surrogate was removed and the infant left in the home cage. These results differ from those previously obtained with mother-reared infants. It is concluded that surrogate-reared infant squirrel monkeys do not show the same separation response or attachment to their rearing figure as do mother-reared infants.     

Partial cytochrome oxidase deficiency without subsarcolemmal accumulation of mitochondria in chronic progressive external ophthalmoplegia

Chronic progressive external ophthalmoplegia (CPEO) associated with proximal myopathy and/or craniosomatic abnormalities is a rare syndrome in which morphological mitochondrial changes have been found in some fibres (subsarcolemmal accumulation of mitochondria or "ragged red" fibres). We report a 14-year-old boy with CPEO and a mild proximal myopathy without these characteristic "ragged red" fibres. Histochemistry of skeletal muscle showed a mosaic of fibres without detectable cytochrome oxidase activity, while other mitochondrial enzymes were normal. The total cytochrome oxidase activity and cytochrome aa3 concentration in muscle mitochondrial fractions were only 40% of normal. This case is unique in that a biochemical defect was not accompanied by morphological abnormalities and may represent an early stage of CPEO before the development of morphological changes, or alternatively, a new variant of the disease.     

传统外侧“L”形切口钢板内固定与跗骨窦小切口微型钢板内固定治疗跟骨骨折患者的效果

目的比较传统外侧“L”形切口钢板内固定与跗骨窦小切口微型钢板内固定治疗跟骨骨折患者的效果。方法选取福建医科大学附属三明第一医院2019年1—12月收治的59例跟骨骨折患者,随机分为试验组(31例)和对照组(28例)。对照组采取外侧“L”形切口钢板内固定治疗,试验组采取跗骨窦小切口微型钢板内固定治疗,比较两组的治疗效果。结果试验组手术时间、住院时间均短于对照组,术中出血量少于对照组,差异有统计学意义(P<0.05);两组骨折愈合时间比较,差异无统计学意义(P>0.05)。术后,两组跟骨结节关节角、Gissane角均大于术前,差异有统计学意义(P<0.05);但组间跟骨结节关节角、Gissane角比较,差异无统计学意义(P>0.05)。试验组并发症发生率低于对照组,Maryfand评分、足部功能恢复优良率均高于对照组,差异有统计学意义(P<0.05)。结论跗骨窦小切口微型钢板内固定治疗跟骨骨折患者的效果优于传统外侧“L”形切口钢板内固定,可有效促进患者足部功能恢复,且安全性较高。