二苯甲酮类防晒剂的结构和安全性研究

目的 通过对二苯甲酮3种衍生物二苯甲酮-1、二苯甲酮-2和二苯甲酮-3进行皮肤光毒实验和皮肤光变态反应,探讨3种不同结构防晒剂对皮肤的安全性.方法 用HyperChem7.0软件分别计算出上3种防晒剂的总能量、结合能、电子能、净电荷、偶极矩等结构参数,用SPSS13.0软件分析不同结构参数和防晒剂皮肤光毒反应指数及光变态反应指数的相关性.结果 光毒反应指数和结构参数中的总能量和电子能呈负相关(P<0.01),并随净电荷之和的增加而上升(P<0.01).光变态反应指数仅和结构参数中的结合能密切相关(P<0.01),和其他结构参数均无相关性(P>0.05).结论 初步确定光毒反应指数和结构总能量、电子能相关.光变态反应指数和结合能相关.从而初步建立结构总能量、电子能、净电荷之和-皮肤光毒反应模型以及结合能-光变态反应模型.     

T淋巴细胞对小鼠接触变应性光皮炎的影响

目的 探讨T淋巴细胞在接触变应性光皮炎(CAPD)发病中的作用.方法 选用BALB/c纯系雌性小鼠建立氯丙嗪所致的CAPD模型,采用腹腔注射抗小鼠CD4、CD8单克隆抗体(mAb)方法,激发后48h测小鼠耳肿度,局部皮损行HE染色,计单一核细胞浸润数.ELISA法测小鼠血清白介素10(IL-10)、干扰素γ(IFN-γ)水平.结果 腹腔注射抗CD4mAb组,IL-10水平与阴性对照组(仅光激发,不做光诱导)接近(P>0.05),而IFN-γ水平较阴性对照组高(P<0.05);与腹腔注射抗CD8mAb组相比,IL-10、IFN-γ水平均降低,但耳肿度及单一核细胞浸润数该两组接近,且该两组IL-10、IFN-γ水平均低于阳性对照组(P<0.05);联合注射抗CD4、CD8mAb组,IL-10、IFN-γ水平,耳肿度及单一核细胞浸润数均接近阴性对照组(P>0.05).结论 在氯丙嗪诱导的CAPD模型中,CD4⁺Th1和CD8⁺T淋巴细胞可通过上调IFN-γ水平正调节该反应;而CD4⁺Th2淋巴细胞可通过上调IL-10水平下调该反应.     

Photoallergic dermatitis from 8-methoxypsoralen

Summary A 36 year old woman with psoriasis vulgaris developed generalized photoallergic dermatitis to 8-methoxypsoralen after 16 uneventful treatments with 8-methoxypsoralen und UVA (PUVA). The diagnosis of photoallergy was confirmed by re-exposure to oral 8-methoxypsoralen and total body UVA irradiation; phototests using topical and oral 8-methoxypsoralen with a high intensity monochromator or with a new high intensity light apparatus for the delivery of UVA; and histological studies. Photoallergy occurred only with UVA, but not with UVB or UVC. There was no photoallergy following trimethylpsoralen.     

An attempt to reduce the photosensitizing potential of chlorpromazine with the simultaneous use of β- and dimethyl-β-cyclodextrins in guinea pigs

Summary The effects of topically applied -cyclodextrin (-CyD) and heptakis(2,6-di-O-methyl)--cyclodextrin (DM--CyD) on photoallergic contact dermatitis due to chlorpromazine hydrochloride (CPZ) were investigated using the photomaximization technique in guinea pigs. From the gross and histological observations, the photosensitizing potential of CPZ with the simultaneous topical use of -CyDs was significantly lower than that of CPZ alone. The alleviating efficacy of DM--CyD was greater than that of -CyD. -CyDs suppressed the penetration of CPZ into the skin through the formation of poorly skin-permeable complexes. In addition, -CyDs inhibited (a) the photoinduced free radicals derived from CPZ in the isolated dry skin and (b) the in vitro photochemical binding of CPZ to bovine serum albumin. These observations suggest that -CyDs suppress the photochemical reactions between CPZ and biological macromolecules present in the skin, resulting in the failure to form a photoantigen. The present results indicate that DM--CyD is particularly effective in alleviating photoallergic contact dermatitis due to CPZ.     

他克莫司软膏治疗光敏性皮肤病临床观察

目的探讨0.1%他克莫司软膏治疗成人光敏性皮肤病的疗效和安全性。方法入选的30例光敏性皮肤病患者均口服昆明山海棠片,皮损一侧外涂他克莫司软膏(试验侧),另一侧外涂复方氧化锌软膏治疗(对照侧),分别于治疗前和治疗后第1,2和4周时各随访1次,评价两组的疗效和安全性。结果治疗结束时,试验侧有效率为93.33%,显著高于对照侧(86.67%),差异有统计学意义(P<0.001),且试验侧皮肤的水分和油脂改善程度及患者的满意度明显优于对照侧,差异有统计学意义(P均<0.05),但弹性无明显改变,差异无统计学意义(P>0.05)。试验侧不良反应(瘙痒、灼痛等)有9例,对照侧不良反应(红斑、瘙痒等)有1例。结论 0.1%他克莫司软膏治疗成人光敏性皮肤病具有良好的疗效和安全性。     

豚鼠和小鼠光变态反应性接触性皮炎模型的建立及比较

目的：比较两种动物作为光变态反应接触性皮炎（ＰＣＡＤ）模型各自的优势,以择其在临床工作中发现潜在的光敏性物质。方法：以６－甲基香豆素（６－ＭＣ）为光敏剂分别制作豚鼠和小鼠ＵＶＡ的ＰＡＣＤ模型,并以外涂６－ＭＣ经ＵＶＢ照射组作为对照。结果;成功的建立了两种动物我涂６－ＭＣ并经ＵＶＡ照射的ＰＡＣＤ模型。