早泄与错配——男女性高潮潜伏期的对照研究

目的:通过对男性与女性性高潮潜伏期的对照研究了解早泄的发病率以及与女性的关系。方法:通过两组(A组来自医院的妇科普通病人,B组来自广播电台的听众)不同的女性样本,采用自行编制的调查问卷进行调查。结果:她们的男性伴侣患重度早泄6%～10%,轻到中度早泄24%～42%;平均男性的射精潜伏期是12分钟,普通女性理想的射精潜伏期是12分钟,但自愿参加调查的女性期望射精潜伏期是18分钟,这种期望越高,就会使她们的性伴侣对自己的射精潜伏期越不满意。40%左右的女性只有一次性高潮,近50%左右的女性可以获得多次性高潮。结论:早泄是一种与女性性功能状态密切相关的疾病诊断,除了严重的早泄是生理或心理障碍之外,造成早泄的另一个重要原因是错配问题。作者建议将女性性高潮潜伏期过长作为性功能障碍与早泄一同诊断和治疗,或许会起到事半功倍的效果。     

Attitude Mismatching: Discrepancies in the Sexual Attitudes of African American Mothers and their Pre-adolescent Children

The present study examined attitude congruence between mothers and their pre-adolescent children regarding the precursors of sexual behavior (i.e., having a boyfriend/girlfriend, dating), timing of sexual initiation (i.e., waiting until marriage to have sex), and the potential consequences of sexual behavior (i.e., the seriousness of pregnancy and AIDS). A sample of 932 fourth and fifth grade African American children and their mothers participated by completing computer-administered surveys. The results indicated significant discrepancies between maternal and child attitudes, particularly regarding the precursors of sexual behavior. In addition, children often inaccurately perceived their mothers’ attitudes, and evidence suggested that lack of sexual communication is associated with some of these misperceptions. The implications of sexual attitude discrepancies between mothers and children for prevention programs are discussed.     

Impact of human leukocyte antigen mismatching on outcomes of liver transplantation: A meta-analysis

AIM:To assess the effect of human leukocyte antigen(HLA) mismatching on liver graft outcome and acute rejection from a meta-analysis of available cohort studies.METHODS:Articles in PubMed/MEDLINE,EMBASE and the Cochrane database from January 1970 to June 2009,including non-English literature identified in these databases,were searched.Only studies comparing HLA or sub-phenotype matching with mismatching were extracted.The percentage of graft survival was extracted by "Engauge Digitizer" from survival curves...     

疏肝养胃通脉冲剂对慢性萎缩性胃炎患者DNA错配基因hMSH2蛋白表达的影响

目的观察疏肝养胃通脉冲剂对慢性萎缩性胃炎(CAG)患者DNA错配基因hMSH2蛋白表达的影响。方法应用免疫组化S-P方法,对174例不同病理亚型CAG患者进行治疗前后hMSH2蛋白表达检测。结果治疗组随着萎缩加重、异型加大和Hp感染程度加深,治疗后下调hMSH2蛋白过表达加大(P<0.05);而对照组治疗前后变化不显著。在化生中,治疗组随肠上皮和假幽门腺化生加重,细胞核hMSH2蛋白过表达下调渐小,呈负相关性,即单纯组最显著(P<0.01),细胞质以中度最显著(P<0.01),重度组虽有下调,但治疗前后比较相关性不显著(P>0.05);对照组细胞核和细胞质hMSH2蛋白过表达有一定的下调,但同组间比较相关性不显著(P>0.05)。结论疏肝养胃通脉冲剂能抑杀Hp,对CAG患者特别是伴异型增生者能下调hMSH2蛋白过表达,对治疗CAG和预防其癌变有明显效果。     

Major histocompatibility complex class I-related chain A allele mismatching, antibodies, and rejection in renal transplantation

Even when kidney allografts are well matched for human leukocyte antigen (HLA) and anti-HLA antibodies are not detected, graft rejection can still occur. There is evidence that some patients who lose their graft have antibodies specific for major histocompatibility complex (MHC) class I-related chain A (MICA) antigens. We investigated whether mismatching MICA alleles associates with MICA antibody production and graft rejection or dysfunction. MICA and HLA antibody screening in 442 recipients was performed, and specificities were confirmed in a subgroup of 227 recipients using single-antigen multiplex technology. For assignment of MICA antibody specificity, we used three independent assays. In addition, MICA alleles of 227 recipients and donors were determined by DNA sequencing. In all, 17 patients (7.5%) had MICA antibodies, and 13 patients (6%) developed MICA donor-specific antibodies (DSA). Multivariate analysis revealed MICA mismatching, as an independent significant factor associated with the presence of MICA antibodies (p = 0.009), and 14 mismatched MICA residues significantly correlated with MICA antibody production. MICA and HLA antibodies significantly associated with acute rejection (AR) and MICA DSA and HLA DSA correlated with decreased graft function by univariate and multivariate analysis. We conclude that mismatching for MICA epitopes in renal transplantation is a mechanism leading to production of MICA antibodies that associate with AR and graft dysfunction.     

Development of a security system for assisted reproductive technology (ART)

PurposeIn the field of assisted reproductive technology (ART), medical accidents can result in serious legal and social consequences. This study was conducted to develop a security system (called IVF-guardian; IG) that could prevent mismatching or mix-ups in ART.ResultsMany of the label paper samples were toxic to mouse embryo development. We selected a particular label paper (P touch label) that did not affect mouse embryo development. The LED lights were non-toxic to the development of the mouse embryos under any experimental conditions. There were no differences in the clinical pregnancy rates between the IG-applied group and the control group (40/104 = 38.5 % and 30/82 = 36.6 %, respectively).ConclusionsThe application of IG in clinical practice did not affect human embryo development or clinical outcomes. The use of IG reduces the misspelling of patient names. Using IG, there was a disadvantage in that each treatment step became more complicated, but the medical staff improved and became sufficiently confident in ART to offset this disadvantage. Patients who received treatment using the IG system also went through a somewhat tedious process, but there were no complaints. These patients gained further confidence in the practitioners over the course of treatment.     

碱基匹配程度对压电传感器基因杂交效应的影响

探讨碱基匹配程度对压电传感器基因杂交效应的影响。方法 :在压电传感器阵列上固定 2 0碱基的巯基DNA探针 ,而后分别与有 0 ,1,2 ,3个碱基不匹配的靶序列进行杂交 ,计算机采集分析传感器频率数据 ,比较各匹配程度下杂交达到平衡所需时间 ,杂交前后频率差 ,并计算杂交动力学参数。结果 :随碱基不匹配数增加 ,杂交时间有缩短趋势 ,杂交前后频率差值逐步减小 ,结合常数明显下降 ,解离常数增大 ,使平衡常数显著减小。结论 :在石英晶体传感器阵列上 ,基因杂交效率随碱基不匹配数量增加而减小。