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排序方式: 共有1457条查询结果,搜索用时 15 毫秒
1.
Gal Ophir Ninette Amariglio Jasmine Jacob-Hirsch Ran Elkon Gideon Rechavi Daniel M. Michaelson 《Neurobiology of disease》2005,20(3):519-718
Apolipoprotein E4 (apoE4), the major genetic risk factor of Alzheimer's disease (AD), is associated with enhanced brain inflammation. Genome-wide gene expression profiling was employed to study the effects of apoE genotype on hippocampal gene expression in LPS-treated mice, transgenic for either apoE4 or the AD benign allele, apoE3. This revealed that the expression of inflammation-related genes following intracerebroventricular injection of LPS was significantly higher and more prolonged in apoE4 than in apoE3 transgenic mice. Clustering analysis revealed gene clusters which responded differently in apoE4 and apoE3 mice and were significantly enriched in NF-kappaB response elements. Direct measurement of NF-kappaB-regulated genes revealed that their extent of activation was greater in the apoE4 mice. Immunohistochemistry experiments revealed that microglial and NF-kappaB activation were more pronounced in apoE4 than in apoE3 mice. These findings suggest that the increased brain inflammation in apoE4 mice is related to disregulation of NF-kappaB signaling pathway. 相似文献
2.
Jeffrey F. Waring Rita Ciurlionis Robert A. Jolly Matthew Heindel Gerry Gagne Jane A. Fagerland Roger G. Ulrich 《Toxicology in vitro》2003,17(5-6):693-701
In vitro human hepatocyte cultures are a key tool in the investigation of xenobiotic toxicity and metabolism. In most in vitro hepatocyte studies, the cells are allowed to adhere to an extracellular matrix, such as collagen. Unfortunately, the ability of freshly isolated hepatocytes to adhere to collagen varies from donor to donor. We used microarray analysis to determine what gene expression differences exist between hepatocytes in suspension and hepatocytes attached to collagen. Results from different donors showed a considerable difference in gene expression patterns between the two hepatocyte populations. In addition, we also compared the gene expression profiles of hepatocytes in culture with liver tissue. The results showed that both hepatocytes in suspension and hepatocytes attached to collagen display significant gene expression differences compared with liver tissue. Finally, we show that both populations of hepatocytes are responsive to dexamethasone and regulate some of the same genes. Overall, our results suggest that either significant gene expression changes occur in isolated hepatocytes or that suspended and attached cells represent different populations of hepatocytes found in intact livers. 相似文献
3.
We previously reported the expression profiles of 9 cytochrome P450 isozymes (CYPs) proteins and those of 40 CYPs genes in pregnant rat's liver, placenta and fetal liver after treatment with pregnenolone-16alpha-carbonitrile (PCN) or phenobarbital (PB). This study was carried out focusing on the gene expression profiles of Phase II drug metabolizing enzymes, Glutathione S-transferase isozymes (GSTs) and UDP-glycosyltransferase isozymes (UDPGTs). Fischer 344 (F344) pregnant rats were daily treated intraperitoneally with 50 mg/kg of PCN or 80 mg/kg of PB from 13 to 16 days of gestation (DG). They were sacrificed on 17 DG, and microarray analysis using Affymetrix Rat Expression Array 230 A was performed. Among 16 GSTs genes examined in this study, 7 genes were significantly induced in dam's liver and 3 genes in fetal liver, respectively, in the PCN-group, while 8 genes were significantly induced in dam's liver and 1 gene in fetal liver, respectively, in the PB-group. On the other hand, among 11 UDPGTs genes examined, 5 genes were significantly induced in dam's liver and 3 genes in fetal liver, respectively, in the PCN-group, while 5 genes were significantly induced in dam's liver and 1 gene in fetal liver, respectively, in the PB-group. There were no significant changes in the placenta of all groups. This is the first report of the gene expression profiles of Phase II drug metabolizing enzymes in pregnant rat and fetal livers and placenta after treatment with typical inducers of drug metabolizing enzymes. 相似文献
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目的:从基因水平研究肝移植急性排斥反应中T淋巴细胞信号传导途径。方法:利用4096条大鼠cDNA克隆的基因芯片对从急性排斥组Wistar→SD(n=5)和对照组SD→SD(n=5)两组肝移植大鼠T细胞中抽提、纯化mRNA,扩增、逆转录成cDNA,荧光标记后与芯片杂交,扫描后筛选出差异表达的基因。结果:在4096个基因中共发现差异表达基因190条,其中ll条与细胞信号传导有关的基因差异表达明显:MHC(3条)、CD3抗原(1条)相关基因;蛋白酶类:蛋白激酶C结合蛋白、蛋白酪氨酸磷酸酯酶D型受体、磷脂酰肌醇二磷酸酶基因各l条;涉及核酸信号传导、激活、转录、翻译的基因3条。结论:T细胞在肝移植术后排斥反应中信号传导机制涉及多个基因,基因芯片技术的大规模筛选为进一步研究T细胞在排斥反应中的信号传导途径提供了客观依据和目标。 相似文献
6.
Alena Jiroutova Rastislav Slavkovsky Martina Cermakova Lenka Majdiakova Irena Hanovcova Radka Bolehovska Milena Hajzlerova Hana Radilova Ema Ruszova Jiri Kanta 《Experimental and toxicologic pathology》2007,58(4):263-273
Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. They differ in their origin and location, and possibly in patterns of gene expression. Normal and carbon tetrachloride-cirrhotic livers from rats were used to isolate HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined. The pattern of gene expression changed during HSC activation and there were distinct differences between HSC and MFB. Little difference between normal cells and cells isolated from cirrhotic liver was found. 相似文献
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Parallel Detection of Bacterial Pathogens in Cerebrospinal Fluid with 16S rRNA Probe Microarray 总被引:1,自引:0,他引:1
Yi LIU Jinxiang HAN Bo ZHU Haiyan HUANG Key Laboratory for Biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center Jinan China 《中华微生物学和免疫学杂志(英文版)》2004,(3)
The most commonly used method for detection ofpathogenic bacteria in cerebrospinal fluid (CSF) speci mens of clinical laboratories is isolation and identificationof the causative agents by cultural method, biochemicaland serological t… 相似文献
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联合应用SSH和cDNA Microarray筛选肺癌相关基因 总被引:3,自引:0,他引:3
目的 利用抑制消减杂交(suppression subtractive hybridization,SSH)和cDNA Microarray 筛选肺癌组织、肺癌旁组织和其它肿瘤组织中相互差异表达的基因。方法 将利用SSH构建的BEP2D细胞永生化阶段、恶性转化前阶段和恶性转化阶段三个cDNA文库中的克隆制作在一张芯片上,筛选了15例肺癌组织、5例肺癌旁组织和其他癌组织24例(肝癌、胃癌、食管癌、乳腺癌、白血病、子宫内膜癌、脑神经胶质瘤和结肠癌各3例)中mRNA的表达差异。结果 获得肺癌组织高于肺癌旁组织表达的cDNA26个,肺癌旁组织高于肺癌组织表达的31个。二者高于其它8种癌组织的分别为:肺癌旁组织中63个,肺癌组织中87个。结论 联合应用SSH和cDNA Microarray是筛选和鉴定不同样本中差异表达基因的快速和有效的方法;肺癌旁组织和肺癌组织中差异表达的基因,以及这二者与其它组织差异表达的基因.不仅可能是肺癌发生发展机制中的重要基因,而且可能是用于肺癌诊断的候选基因。 相似文献