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1.
The nonstinging house ant, Monomorium pharaonis (pharaoh ant), was recently identified as a cause of respiratory allergy. This study was performed to evaluate the extent of sensitization to pharaoh ant, and its clinical significance in asthmatic patients. We carried out skin prick tests in 318 patients with asthma. Specific IgE (sIgE) to pharaoh ant was measured by ELISA, and cross-reactivity was evaluated by ELISA inhibition tests. Bronchial provocation testing was performed using pharaoh ant extracts. Fifty-eight (18.2%) of 318 patients showed positive skin responses to pharaoh ant, and 25 (7.9%) had an isolated response to pharaoh ant. Positive skin responses to pharaoh ant were significantly higher among patients with non-atopic asthma than among those with atopic asthma (26.0% vs. 14.9%, p<0.05). There was significant correlation between sIgE level and skin responses to pharaoh ant (rho=0.552, p<0.001). The ELISA inhibition tests indicated that pharaoh ant allergens had various pattern of cross-reactivity to house dust mites and cockroaches. Bronchial provocation tests to pharaoh ant were conducted for 9 patients, and eight showed typical asthmatic reactions. In conclusion, pharaoh ant is an important source of aeroallergens, and it should be included in the skin test battery for screening the causative allergens in patients with asthma. 相似文献
2.
背景 研究表明胸腺基质淋巴细胞生成素(TSLP)为类白细胞介素(IL)-17的炎性细胞因子,在多种变应性疾病的发生和发展中发挥关键作用,但是尚未见到关于TSLP是否参与中国常见变应原诱导的变应性结膜炎的报道. 目的 研究TSLP和IL-4在由中国常见的黄花蒿诱导的变应性结膜炎小鼠模型眼表组织和颈部淋巴结中的表达变化,探讨TSLP和IL-4在变应性结膜炎发病中的作用. 方法 采用随机数字表法将6~8周龄雌性BALB/c小鼠20只随机分为正常对照组和模型组.将400μl黄花蒿花粉提取液溶解于400μl变应原(抗原)溶媒中制备成抗原溶液,将50μl抗原溶液注射于模型组小鼠足底皮垫下初次致敏,于注射后第10 ~12天用黄花蒿花粉提取液局部点右眼再次致敏,每日点眼1次;正常对照组小鼠未做任何处理.于造模后第13天采用颈椎脱臼法处死小鼠并摘取眼球,在眼科显微镜下各组分别取16只小鼠32只眼角膜上皮、结膜和颈部淋巴结组织,采用实时定量PCR法检测各种靶组织中TSLP mRNA及IL-4 mRNA的相对表达量,另每组各取4只小鼠8只眼眼睑及眼球制备石蜡切片,采用免疫组织化学法检测角膜、结膜及结膜下组织中TSLP和IL-4蛋白的表达. 结果 模型组小鼠第2次致敏后0.5h可见小鼠眼睑水肿、结膜充血水肿、溢泪、搔抓眼睑等症状,症状持续6 ~24 h,造模成功率为80%.实时定量PCR结果显示,模型组与正常对照组小鼠角膜上皮均未发现IL-4表达.模型组小鼠角膜上皮、结膜组织和颈部淋巴结组织中TSLP mRNA表达量分别是正常对照组的(1.63±0.20)、(2.71±0.48)和(1.48±0.05)倍,差异均有统计学意义(=4.44、14.16、5.01,均P<0.05);模型组小鼠结膜和颈部淋巴结中IL-4 mRNA表达量分别是正常对照组的(2.94±0.39)倍和(1.74±0.09)倍,差异均有统计学意义(t=9.92、14.54,均P<0.05).免疫组织化学法检测显示,正常对照组小鼠角膜上皮和结膜上皮细胞中TSLP蛋白表达强度微弱,模型组小鼠TSLP蛋白表达增强.模型组小鼠结膜下组织中可见IL-4呈强阳性表达,正常对照组小鼠结膜下组织中未发现IL-4表达.结论 TSLP主要表达于变应性结膜炎小鼠的角膜、结膜和颈部淋巴结组织,提示其参与变应性结膜炎角膜和结膜的炎症反应;IL-4主要表达于变应性结膜炎小鼠结膜下组织中,提示其主要参与结膜的炎症反应,TSLP和IL-4共同参与变应性结膜炎的发生和发展过程,这2种炎症因子为变应性结膜炎的临床治疗提供了新的靶点. 相似文献
3.
皮肤点刺试验在儿童呼吸道常见变态反应性疾病防治中的意义 总被引:1,自引:0,他引:1
目的探讨柳州地区儿童皮肤变应原试验在变态反应性疾病中的分布及临床意义,为预防和治疗儿童变应性疾病提供科学依据。方法选择患有支气管哮喘和(或)变态反应性鼻炎的患儿110例,男性66例,女性44例,平均年龄(5.35±2.62)岁。变态反应性鼻炎28例,支气管哮喘40例,支气管哮喘合并变态反应性鼻炎42例。所有对象均选用14种变应原进行皮肤变应原点刺试验(SPT),组胺作阳性对照,生理盐水作阴性对照。结果变应原点刺试验总阳性检出率为75.5%(83/110),粉尘螨74.5%(82/110)、屋尘螨71.8%(79/110)、室内霉菌Ⅱ66.4%(73/110)、干草尘埃23.6%(26/110)、犬毛20.9%(23/110)、猫毛17.3%(19/110)、柳17.3%(19/110)、虾10.9%(12/110)、牛奶10.0%(11/110)、鸡蛋8.2%(9/110)、鲤鱼5.5%(6/110)、牛肉4.5%(5/110)、鸡肉1.8%(2/110)、猪肉1.8%(2/110)。>5岁组吸入性变应原阳性率显著高<5岁组(χ2=15.16,P<0.05),<5岁(含5岁)组食物性变应原阳性率高于>5岁组(χ2=12.85,P<0.05)。鼻炎、哮喘、哮喘并鼻炎患儿均以螨类过敏最为常见,不同疾病组间变应原阳性率差异无统计学意义(P>0.05)。结论过敏性因素是儿童变态反应性疾病发病的重要诱发因素,在柳州地区变态反应性疾病儿童可以通过SPT检查明确过敏原,最常见的变应原是粉尘螨、屋尘螨、动物皮毛类、霉菌及干草尘埃类等。随着年龄增长,变态反应性疾病患儿对吸入性变应原更为敏感。鼻炎、哮喘、哮喘并鼻炎患儿有共同的变应原。研究不同年龄段变态反应性疾病患者的变应原特征,有助于对疾病的早期诊断和早期干预。 相似文献
4.
Insights in particulate matter‐induced allergic airway inflammation: Focus on the epithelium
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K. C. De Grove S. Provoost G. G. Brusselle G. F. Joos T. Maes 《Clinical and experimental allergy》2018,48(7):773-786
Outdoor air pollution is a major environmental health problem throughout the world. In particular, exposure to particulate matter (PM) has been associated with the development and exacerbation of several respiratory diseases, including asthma. Although the adverse health effects of PM have been demonstrated for many years, the underlying mechanisms have not been fully identified. In this review, we focus on the role of the lung epithelium and specifically highlight multiple cytokines in PM‐induced respiratory responses. We describe the available literature on the topic including in vitro studies, findings in humans (ie observations in human cohorts, human controlled exposure and ex vivo studies) and in vivo animal studies. In brief, it has been shown that exposure to PM modulates the airway epithelium and promotes the production of several cytokines, including IL‐1, IL‐6, IL‐8, IL‐25, IL‐33, TNF‐α, TSLP and GM‐CSF. Further, we propose that PM‐induced type 2‐promoting cytokines are important mediators in the acute and aggravating effects of PM on airway inflammation. Targeting these cytokines could therefore be a new approach in the treatment of asthma. 相似文献
5.
【摘要】 目的 分析化妆品相关皮肤不良反应的临床特点及主要致病成分,为化妆品不良反应起到预警作用并提供客观的风险评估依据。方法 收集2018年3月至2019年10月在重庆市中医院门诊就诊的512例化妆品不良反应可疑患者,男14例,女498例,通过填写统一制定的化妆品不良反应报告卡,记录患者的病史资料和所使用化妆品的相关信息;对其中103例(男3例,女100例)进行化妆品原物斑贴试验及化妆品成分斑贴试验,结合48和72 h斑贴试验结果综合判定并汇总分析。结果 512例可疑化妆品不良反应病例中,主要表现类型为接触性皮炎(495例,96.7%)。化妆品不良反应的皮损形态主要为红斑501例(97.9%)、丘疹313例(61.1%)、水肿249例(48.6%)、鳞屑166例(32.4%);症状主要为瘙痒480例(93.8%),其次为灼热感359例(70.1%)和紧绷感297例(58.0%)。103例化妆品成分斑贴试验显示,阳性71例,最易引起化妆品不良反应的变应原分别为硫柳汞(31例,30.1%)、十二烷基硫酸钠(29例,28.2%)、秘鲁香脂(17例,16.5%)、布罗波尔(12例,11.7%)及三乙醇胺(10例,9.7%)。将化妆品变应原系列分为14个类别,阳性率前4位的类别分别为乳化剂54例(45.8%)、防腐剂47例(39.8%)、芳香剂17例(14.4%)和表面活性剂10例(8.5%)。2例男性和69例女性斑贴试验阳性,男女阳性率差异无统计学意义(2/3比69/100,χ2 = 0.01,P > 0.05);18 ~ 29岁组、30 ~ 49岁组及50 ~ 70岁组阳性率分别是34%、34%、32.4%,各组间阳性率差异无统计学意义(χ2 = 0.693,P > 0.05)。结论 化妆品不良反应最常见的表现为接触性皮炎,致病成分具有多样性,最易引起化妆品皮肤不良反应的变应原分别为硫柳汞、十二烷基硫酸钠、秘鲁香脂、布罗波尔及三乙醇胺。 相似文献
6.
Jinyoung Yang Hyeyoung Lee Ae Ran Choi Ki Hyun Park Ji Hyeong Ryu 《Scandinavian journal of clinical and laboratory investigation》2013,73(7-8):606-612
AbstractIn vitro allergen-specific immunoglobulin E (sIgE) measurement has been used as an important diagnostic tool for allergic diseases. Currently, quantitative sIgE levels are detected mainly by using ImmunoCAP and Immulite 2000 assay system. These two systems have the same calibration scale at 0–100 kUA/L, but they differ in used allergens, detection methods and automation systems. We compared 1600 paired sIgE results for 204 allergic patients, including 100 paired sIgE assay results for each of 16 allergens (Alternaria alternata, birch-alder mix, cat dander, D. farinae, D. pteronyssinus, dog dander, buckwheat, crab, egg white, mackerel, milk, peach, peanut, shrimp, soybean and wheat flour). Inter-method comparison was performed for qualitative data with a cutoff of 0.35 kUA/L and a detection limit of 0.1 kUA/L, semi-quantitative class results and quantitative data. In qualitative comparisons, the overall concordance rate ranged from 81.0% to 99.0% (k: 0.599–0.949) with the cutoff value of 0.35 kUA/L. It also ranged from 80.0% to 99.0% (k: 0.521–0.951) with the detection limit of 0.1 kUA/L. The class results from these two assays showed good agreements for all allergens. For quantitative sIgE results, these two assays showed moderate positive correlations for Dog dander (r?=?0.683) and Mackerel (r?=?0.573) but high to very high correlations for the other 14 allergens (r?=?0.734–0.972). Immulite 2000 and ImmunoCAP assays demonstrated good concordance and correlation for 16 common allergens, but international standards against each specific allergen for calibration and harmonization of sIgE tests are still needed. 相似文献
7.
Saroj Gupta Rakesh Kumar Bidani Shashi Jhamb Mahendra Kumar Agarwal 《The Journal of asthma》1996,33(5):339-348
The etiological significance of animal danders in Indian patients with nasobronchial disorders has not yet been investigated. In the present study, the role of animal danders in the etiology of bronchial asthma was studied. Extracts of danders from 6 animals along with guinea pig whole pelt were prepared. Intradermal and bronchial provocation tests with these extracts were performed on (i) 68 asthmatics and (ii) 20 nonallergic healthy volunteers. In patients, significant positive skin reactions (2+ to 4+) ranged from 1.4% each with guinea pig whole pelt and ox dander to 8.8% with dog dander extracts. None of the healthy volunteers elicited such a response. On bronchial provocation, 20% and 53.8% of the tests were positive in asthmatics showing 1 +and 2+skin reactivity, respectively. All the patients as well as controls eliciting negative intradermal responses demonstrated uniformly negative bronchial provocation tests to different dander extracts. Analysis of various clinical features of asthmatics with respect to skin positivity to dander/pelt extract was also carried out. Radioallergosorbent tests (RASTs) were performed to estimate dander-specific IgE levels in the sera of patients showing different grades of skin response to dander extracts of dog, horse, and goat. Sixty percent of sera from the patients showing 2+ to 4+ skin reactivity to various animal dander extracts showed positive RASTs. RAST positivity as well as RAST ratio increased with increase in the intensity of skin response. All the patients with positive cutaneous as well as positive bronchial responses also showed positive RASTs. Similarly, all the patients with positive skin and positive RASTs showed positive bronchoprovocation tests. These results suggested that animal danders play an important role in the etiology of bronchial asthma. Some of the clinical characteristics of asthmatics, such as (i) early age at onset of asthma, (ii) positive family history, and (iii) asthma with associated allergies, have significant bearing on the cutaneous response to various dander extracts. 相似文献
8.
《Allergologia et immunopathologia》2020,48(6):597-602
Introduction and ObjectivesMoths are a significant source of indoor and outdoor aeroallergens. High prevalence of IgE-mediated sensitization was demonstrated in a group of patients with allergic respiratory diseases. There are no studies on adult stage of these moth species allergens involved in allergic respiratory reactions - the aim of this study.Material and Methods36 participants were included in an experimental study, submitted to skin prick test with Bombyx mori wing extract and six other common allergens, as well as Western blot analysis with incubated nitrocellulose membrane impregnated with silkworm moth extract and human IgE-antibody. The participants were divided into 3 groups: 1) 21 allergic patients whose skin prick test was positive to Bombyx mori wing extract, 2) eight allergic patients whose skin prick test was positive to mite and negative to Bombyx mori extract 3) seven negative non-allergic subjects.ResultsAmong the 21 participants from group 1, 19 serum samples reacted to Bombyx mori extract by Western blot. All of them reacted to a protein at 80 kDa and five other proteins (66, 50, 45, 37 and 30 kDa) were identified in more than 50% of the individuals tested, considered as major allergenic proteins. Sera from seven out of eight patients sensitized to house dust mite demonstrated IgE-reactivity to Bombyx mori extract by Western blot analysis. Serum samples from healthy participants did not react at all.ConclusionSix major reactive proteins by immunoblot analysis from moth’s wings sensitized patients can be potential allergens. The one at 80 kDa is the major protein, seen in all IgE-reactive patients from group 1 and in none from group 2, yet to be identified. Future studies should be conducted to better characterize these proteins. 相似文献
9.
目的了解支气管激发试验阳性患者对14种吸入变应原(过敏原)过敏的分布状况和相关性。方法选取366例支气管激发试验阳性患者进行变应原皮试。结果366例患者中297例对14种变应原试验阳性,总阳性率为81.1%;过敏原以屋尘、粉尘螨、多价昆虫、春季花粉I等为主;其中对6种及6种以上变应原过敏的患者占35.7%。皮试阳性组患者与皮试阴性组患者吸人使第一秒用力呼气容积(FEV1)下降20%的乙酰甲胆碱累积剂量(PD20FEV,)差异有统计学意义(P〈0.05)。结论支气管激发试验阳性患者最常见的过敏原为屋尘、粉尘螨、多价昆虫、春季花粉I等;PD20FEV。与变应原皮试检出阳性率可能存在相关性。 相似文献
10.
R. Kalmarzi P. Ataee Gh. Homagostar M. Tagik E. Ghaderi W. Kooti 《Allergologia et immunopathologia》2018,46(1):45-57