全文获取类型
收费全文 | 795篇 |
免费 | 83篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 12篇 |
儿科学 | 294篇 |
妇产科学 | 14篇 |
基础医学 | 161篇 |
口腔科学 | 6篇 |
临床医学 | 34篇 |
内科学 | 20篇 |
皮肤病学 | 8篇 |
神经病学 | 9篇 |
特种医学 | 23篇 |
外科学 | 73篇 |
综合类 | 31篇 |
预防医学 | 4篇 |
眼科学 | 29篇 |
药学 | 13篇 |
中国医学 | 2篇 |
肿瘤学 | 155篇 |
出版年
2024年 | 6篇 |
2023年 | 28篇 |
2022年 | 23篇 |
2021年 | 22篇 |
2020年 | 42篇 |
2019年 | 36篇 |
2018年 | 26篇 |
2017年 | 24篇 |
2016年 | 28篇 |
2015年 | 29篇 |
2014年 | 43篇 |
2013年 | 63篇 |
2012年 | 36篇 |
2011年 | 29篇 |
2010年 | 30篇 |
2009年 | 29篇 |
2008年 | 29篇 |
2007年 | 34篇 |
2006年 | 30篇 |
2005年 | 21篇 |
2004年 | 27篇 |
2003年 | 20篇 |
2002年 | 29篇 |
2001年 | 22篇 |
2000年 | 11篇 |
1999年 | 14篇 |
1998年 | 15篇 |
1997年 | 18篇 |
1996年 | 10篇 |
1995年 | 14篇 |
1994年 | 13篇 |
1993年 | 11篇 |
1992年 | 5篇 |
1991年 | 8篇 |
1990年 | 11篇 |
1989年 | 6篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 2篇 |
排序方式: 共有888条查询结果,搜索用时 15 毫秒
1.
Objective To evaluate the sensitivity and specificity of regularly used immunohistochemical markers, including Vimentin (Vim), Desmin (Des), Myoglobin (MG), Myosin (MS), Smooth-muscle actin (SMA) and Sarcomeric actin ( Sr-A ) , in the diagnosis of rhabdomyosarcoma (RMS). Methods After resection, 24 RMSs and other childhood tumor specimens were fixed in 10% neutral-buffered formalin and embeded in paraffin. The immunohistochemical staining was performed by LSAB procedure. Heat-induced epitope retrieval of Des, MS, Sr-A was processed in order to enhence positive rate and positive strength. Results Vim, MG, MS, Des, Sr-A, SMA were arranged in the order of sensitivity from higher to lower. About specificity, Sr-A, Des, SMA, MG, Vim standed in a sequence from higher to lower ( the data of MS is insufficient) ; Des, MG, Sr-A possessedhigher experimental efficiency, followed by SMA, Vim in a succession. Conclusion Vim and MG are of the higher sensitivity but lower specificity. On the reverse, Sr-A and Des hoM the better specificity but lower sensitivity. So the combination of multiple antibody reactions shouM be considered to improve the diagnostic ability in poorly differentiated RMS. According to the result of experimental efficiency, we suggest that the combination of Des, MG and Sr-A can make it possible to diagnose the majority of RMS clearly. 相似文献
2.
B n dicte Brichard Jacques Ninane Serge Gosseye Christine Verellen-Dumoulin Christiane Vermylen Jean Rodhain Guy Comu 《Pediatric hematology and oncology》1991,8(3):215-219
A 9-year-old boy presented with a small subcutaneous tumor of the trunk and diffuse bone marrow involvement. The first histological diagnosis given was undifferentiated malignancy possibly of neural crest origin and chemotherapy was started immediately using vincristine, cyclophosphamide, cisplatin, and teniposide (OPEC). Complete response was achieved after four courses of chemotherapy. Histological slides were then reviewed and the final diagnosis of alveolar rhabdomyosarcoma (RMS) was retained. Moreover, chromosome analysis of malignant cells in the bone marrow revealed a translocation involving chromosomes 2 and 13:t(2;13) (q35;q14). This specific karyotype finding has been recently reported in a few cases and could be specific for alveolar RMS. The patient had a relapse 7 months after diagnosis and died 4 months later. 相似文献
3.
Laryngeal pleomorphic rhabdomyosarcoma 总被引:2,自引:0,他引:2
M. U. Akyol B. Sözeri T. Küçükali O. Ög˘retmenog˘lu 《European archives of oto-rhino-laryngology》1998,255(6):307-310
A case of an extremely unusual tumor of the larynx, pleomorphic rhabdomyosarcoma, is presented with a review of literature.
This is the fifth case of this malignancy described in the larynx in the English language literature. A histopathological
diagnosis was made with immunohistochemistry and electron microscopy. In contrast to other reported cases, the tumor in the
present case had a very aggressive behavior. Despite radical surgery involving total laryngectomy and neck dissection followed
by radiation therapy, the patient died of disease 8 months following treatment.
Received: 14 March 1997 / Accepted: 23 January 1998 相似文献
4.
Bhattacharya B Sariya D Reddy VB Kluskens L Gould VE Gattuso P 《Diagnostic cytopathology》2002,26(6):384-386
A 4(1/2)-yr-old female presented with right-sided pleural effusion and a retroperitoneal mass. Cytologic analysis of the pleural fluid yielded malignant small round blue cells, which were noncohesive, 3-4 times the size of lymphocytes. The malignant cells had hyperchromatic, pleomorphic nuclei with moderate amounts of vacuolated cytoplasm. A few fiber-shaped cells were also seen. Immunostains for desmin, muscle-specific actin were positive; ultrastructural findings of thick and thin actin-myosin filaments confirmed the diagnosis of embryonal rhabdomyosarcoma. This case illustrates the importance of performing appropriate immunohistochemical stains and ultrastructural studies on cytological material to arrive at a definitive diagnosis. 相似文献
5.
Yosuke Morimitsu Hiroshi Tanaka Shigeaki Iwanaga Masamichi Kojiro 《Pathology international》1993,43(4):204-208
A rare case of an alveolar soft part sarcoma of the uterine cervix in an 8 year old girl is presented. The patient was admitted because of genital bleeding lasting for 7 months. A polypoid tumor, 2times1.5 cm in diameter, was found in her external uterine os and was surgically resected. Microscopically, the tumor consisted of a uniform sheet of tumor cells in the cytoplasm which contained granules and which were stained with periodic acid-Schiff, both before and after the diastase digestion. Alveolar arrangement of the tumor cells was manifested with reticulin silver impregnation. Dense, membrane bound granules were evident at an ultrastructural level in the cytoplasm of the tumor cells. An immunohistochemical examination demonstrated a positive reaction for anti-desmin, anti-myoglobin, anti-HHF35 and anti-neuron specific enolase in the cytoplasm. 相似文献
6.
7.
Enterovirus 71 (EV71) is a highly infectious major causative agent of hand, foot, and mouth disease (HFMD) which could lead to severe neurological complications. There is currently no effective therapy against EV71. In this study, RNA interference (RNAi) is employed as a therapeutic approach for specific viral inhibition. Various regions of the EV71 genome were targeted for inhibition by chemically synthesized siRNAs. Transfection of rhabdomyosarcoma (RD) cells with siRNA targeting the 3'UTR, 2C, 3C, or 3D region significantly alleviated cytopathic effects of EV71. The inhibitory effect was dosage-dependent with a corresponding decrease in viral RNA, viral proteins, and plaque formations by EV71. Viral inhibition of siRNA transfected RD cells was still evident after 48 h. In addition, no significant adverse off-target silencing effects were observed. These results demonstrated the potential and feasibility for the use of siRNA as an antiviral therapy for EV71 infections. 相似文献
8.
Xuejin Ma ME Xiaoxi Chen MD Guoyuan Jiang MBBS Lin Jiang MD Tingchao Li MBBS Ling Wei MBBS Shiguang Li MD 《The breast journal》2021,27(12):890-894
Rhabdomyosarcoma (RMS) is a common malignancy in children, but embryonal rhabdomyosarcoma (ERMS) deposits rarely occur in the breast in adults. Therefore, little is known about magnetic resonance imaging (MRI) features of breast metastases from RMS, especially the embryonal type. We reported a case of a 22-year-old woman who was diagnosed with ERMS at left foot 2 years ago and accepted operation and chemotherapy. She was confirmed to have breast metastases from the left foot. Successive imaging examinations were performed 3 months apart. Breast ultrasound indicated a benign lesion, and further examination did not reveal any bone metastases. However, predominant restricted diffusion and rim contrast enhancement on MRI combined with the patient's medical history suggested a malignancy of BI-RADS 5. After 3 months, breast ultrasound revealed masses detected last time became larger and lobulated. In addition, internal heterogeneous intensity and rim contrast enhancement with restricted diffusion were revealed on MRI. We speculated that typical MRI findings of breast metastases from RMS may include iso- to hypointensity on T1WI, heterogeneous hyperintensity on T2WI, and circular enhancement with restricted diffusion. Moreover, mild peritumoral edema, rapid expansion of necrosis, and ascending time-intensity curve detected on MRI may be features of the ERMS type. 相似文献
9.
The field of molecular genetics continues to see an ever increasing number of applications to pediatric tumor analysis. Studies
in pediatric tumors have identified novel genes and other genetic changes, a large number of which reflect one of the following
mechanisms: (1) activation of proto-oncogenes; (2) loss of tumor suppressor genes; or (3) creation of novel fusion proteins. At least one of these mechanisms is operational in each of the following pediatric tumors:
neuroblastoma, Ewing sarcoma and peripheral primitive neuroectodermal tumor (pPNET), intra-abdominal desmoplastic small-cell
tumor, rhabdomyosarcoma, synovial sarcoma, and Wilms tumor. Out of this research has come not only an increased understanding
of oncogenesis but also, for each of the tumors listed above, diagnostic and/or prognostic markers that can be used by the
pathologist and oncologist to improve overall patient management.
Received November 20, 1997; accepted April 20, 1998. 相似文献