三磷酸腺苷发光法在耐多药结核分枝杆菌诊断中的运用

目的通过与罗氏固体培养法比较,评估三磷酸腺苷发光法检测耐多药结核分枝杆菌的可行性。方法采用三磷酸腺苷发光法与罗氏固体培养法同时检测和分析149例临床分离的结核分枝杆菌。结果三磷酸腺苷发光法与罗氏固体培养法的一致率为92．6％（138／149）,差异无统计学意义（X^2=0．57,P=0．45）。三磷酸腺苷发光法检测时间为（6．6±2．1）d,明显快于传统罗氏固体培养法的28d（t=422．7,P〈0．001）。结论与常规检测方法比较,三磷酸腺苷发光法具有快速、简便、准确性高等优点,对耐多药结核患者的早期诊断和耐药结核分枝杆菌流行的控制有很大帮助,适合实验室开展。     

快速扩弓结合方丝弓及前方牵引矫治骨性Ⅲ类错的临床分析

目的:探讨快速扩弓结合方丝弓技术及前方牵引装置治疗骨性Ó类错的方法和特点。方法:选择15例骨性Ó类错患儿,男6例,女9例,年龄815~12岁,均为替牙期。X线片显示主要为上颌骨发育不足。左手腕骨片显示为生长发育的高峰前期。结果:15例患者经平均9月的治疗,面型均明显改善,头影测量其骨骼改变主要为 SNA角增大,A点前移。结论:三者联合治疗骨性Ó类错可取得明显的骨骼及侧貌改变。     

First attempt to validate the gSG6‐P1 salivary peptide as an immuno‐epidemiological tool for evaluating human exposure to Anopheles funestus bites

Objective The development of a biomarker of exposure based on the evaluation of the human antibody response specific to Anopheles salivary proteins seems promising in improving malaria control. The IgG response specific to the gSG6‐P1 peptide has already been validated as a biomarker of An. gambiae exposure. This study represents a first attempt to validate the gSG6‐P1 peptide as an epidemiological tool evaluating exposure to An. funestus bites, the second main malaria vector in sub‐Saharan Africa. Methods A multi‐disciplinary survey was performed in a Senegalese village where An. funestus represents the principal anopheline species. The IgG antibody level specific to gSG6‐P1 was evaluated and compared in the same children before, at the peak and after the rainy season. Results Two‐thirds of the children developed a specific IgG response to gSG6‐P1 during the study period and – more interestingly – before the rainy season, when An. funestus was the only anopheline species reported. The specific IgG response increased during the An. funestus exposure season, and a positive association between the IgG level and the level of exposure to An. funestus bites was observed. Conclusions The results suggest that the evaluation of the IgG response specific to gSG6‐P1 in children could also represent a biomarker of exposure to An. funestus bites. The availability of such a biomarker evaluating the exposure to both main Plasmodium falciparum vectors in Africa could be particularly relevant as a direct criterion for the evaluation of the efficacy of vector control strategies.     

Multiorgan retrieval and preservation of the thoracic and abdominal organs in Maastricht III donors

This editorial describes the indications and technical aspects of the simultaneous retrieval of thoracic and abdominal organs in Maastricht III donors as well as the preservation of such organs until their implantation.