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D. Yin J.W. Ding J. Shen L. Ma M. Hara A.S. Chong 《American journal of transplantation》2006,6(1):60-68
Early graft failure following intraportal islet transplantation (IPIT) represents a major obstacle for successful islet transplantation. Here, we examined the role of islet emboli in the induction of early graft failure and utilized a strategy of ischemic-preconditioning (IP) to prevent early islet destruction in a model of syngeneic IPIT in STZ-induced diabetic mice. Numerous focal areas of liver necrosis associated with the islet emboli were observed within 24 h post-IPIT. Pro-inflammatory cytokines, IL-1beta and IL-6, were significantly increased 3 h after IPIT, while TNF-alpha was elevated for up to 5 days post-IPIT. Caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling positive cells were observed in the transplanted islets trapped in areas of necrotic liver at 3 h and 1 day post-IPIT. Hyperglycemia was corrected immediately following IPIT of 200 islets, but recurrence of hyperglycemia was observed within 14 days associated with a poor response to glucose challenge. IP, a procedure of pre-exposure of the liver to transient ischemia and reperfusion, protected the liver from embolism-induced ischemic injury and prevented early islet graft failure. These data suggest that islet embolism in the portal vein is a major cause of functional loss following IPIT that can be prevented by liver IP. 相似文献
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缺血预处理对大鼠肝脏缺血再灌注损伤的保护作用 总被引:1,自引:0,他引:1
目的 探讨缺血预处理(IPC)对肝脏缺血再灌注(I/R)损伤中细胞凋亡和Fas蛋白表达的影响.方法 60只SD大鼠随机分成正常对照组(N组)、缺血再灌注对照组(I/R组)、缺血预处理组(IPC组).比较各组血清AST、ALT和LDH水平,肝组织中凋亡细胞及细胞凋亡指数,肝组织Fas蛋白的表达情况.结果 IP组血清AST、ALT和LDH水平、细胞凋亡指数、Fas蛋白表达低于I/R组.IPC组血清ALT水平高于N组.结论 缺血预处理对肝脏缺血再灌注损伤有保护作用,可能与抑制Fas蛋白高表达、减少细胞凋亡有关. 相似文献
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