异种动物血清诱导的大鼠肝纤维化模型及其机制

研究建立免疫性肝纤维化模型，并探讨该模型形成过程中的形态变化特点及模型形成的始动机制。Wistar大鼠腹腔注射猪血清诱导免疫性肝纤维化模型，以光镜和电镜观察肝纤维化不同阶段的形态变化，并计数嗜酸性粒细胞（Eos）和肥大细胞，以免疫荧光法检测肝内IgG和补体C3。结果：①猪血清注射8周后均形成肝纤维化；②肝纤维化形成过程中除肝星状细胞（HSC，原称Ito细胞）增多外，尚发现原始间叶细胞（PMC）增多、活化；③实验早期汇管区及间隔内Eos和肥大细胞增多，IgG和C3阳性，停止注射后逐渐减弱。结论：本模型用于肝纤维化过程的研究较为理想，其发生可能与异种血清引起的Ⅰ型变态反应中迟发相反应有关；除HSC外，原始间叶细胞也是纤维生成细胞的重要来源。     

Study of heteroseruminduced rat liver fibrosis model and its mechanism

AIM: To investigate the morphological changes in the process of heteroserum induced rat liver fibrosis and the mechanism of fibrogenesis of this model.METHODS: A model of heteroserum-induced rat liver fibrosis was established by intraperitoneal injection of porcine serum. In addition to the observation of the morphological changes of this model, the infiltration of eosinophils and mast cells were measured quantitatively and the deposition of IgG and complement C₃ was detected by immunofluorescence.RESULTS: The rat liver fibrosis was induced successfully at the end of the 8th week after the injection of heteroserum. Besides the increase of hepatic stellate cells (HSC) during the process of liver fibrosis, proliferation and activation of primary mesenchyma cells (PMCs) were also found. In the early stage, the infiltration of eosinophils and mast cells was significantly increased and the deposition of IgG and complement C₃ was positive in the portal tracts and septa, while gradually reduced after the injection was stopped.CONCLUSIONS: This model is suitable for the research on liver fibrogenesis; the pathogenesis of this model may be related with the allergen-induced late phasereaction (LPR) caused by the injection of heteroserum, and the HSCs and the PMCs are important sources of ECM-producing cells.     

Study of heteroserum-induced rat liver fibrosis model and its mechanism

ＳｔｕｄｙｏｆｈｅｔｅｒｏｓｅｒｕｍｉｎｄｕｃｅｄｒａｔｌｉｖｅｒｆｉｂｒｏｓｉｓｍｏｄｅｌａｎｄｉｔｓｍｅｃｈａｎｉｓｍＨＵＡＮＧＺｈｉＧａｎｇ，ＺＨＡＩＷｅｉＲｏｎｇ，ＺＨＡＮＧＹｕｅＥａｎｄＺＨＡＮＧＸｉｕＲｏｎｇＳｕｂｊｅｃｔｈｅａ...