经穹隆结膜入路埋线法重睑成形术应用探讨

目的:通过分析重睑形成原理,综合比较多种重睑成形的方法,讨论埋线法重睑成形术的优点和可行性。方法:对387例患者行经穹隆结膜入路间断缝合埋线法重睑术。其中88例为其他方法埋线后重睑线消失的患者。结果:手术方法可靠,术后所形成的重睑外形良好,重睑维持时间长。最长随访时间38个月。结论:此方法因符合重睑形成原理,可以充分的将较多的上睑提肌腱膜与上睑皮肤结扎固定、故术后所形成的重睑牢固。且在直视下经穹隆结膜入路,降低了对眼球组织损伤的危险性。是埋线法重睑成形术一种可靠的手术方法。     

转录信号传导子和激活子3信号传导通路调控选择性环氧化酶2抑制剂抗结肠癌的机制

目的探究转录信号传导子和激活子3(Stat3)信号传导通路与选择性环氧化酶2(COX-2)抑制剂抗结肠癌细胞株HT-29机制的关系,明确COX-2抑制剂抗结肠癌细胞内信号传导机制。方法将选择性COX-2抑制剂NS-398,作用于结肠癌细胞系HT-29,运用MTT法检测细胞增殖状态;流式细胞仪观察NS-398对细胞凋亡的影响,进一步用RT-PCR检测药物作用前后HT-29中COX-2mRNA的表达;ELISA法测定体系前列腺素E2(PGE2)水平;Westernblot检测药物作用前后Stat3通路相关蛋白JAK2、Stat3的磷酸化活性和cyclinD1、Bcl-2的表达。结果结肠癌细胞系HT-29中COX-2mRNA呈高表达,NS-398呈时间、剂量依赖性方式抑制HT-29细胞增殖,促进其凋亡。NS-398使HT-29细胞COX-2mRNA和PGE2表达水平显著下降。同时p-JAK2、p-Stat3、cyclinD1、Bcl-2表达水平随作用时间延长而下降。结论癌基因Stat3信号传导通路调控了NS-398抗结肠癌的细胞内信号传导机制,最终通过其下游靶基因cyclinD1、Bcl-2影响结肠癌细胞系HT-29的增殖与凋亡。     

Transducers for foot pressure measurement: survey of recent developments

Recent advances in the development of transducers for the measurement of vertical and shear forces acting on the plantar surface of the foot are reviewed. Barefoot and in-shoe discrete and matrix transducers are reviewed in terms of structure, operation, performance and limitations. Examples of capacitive, piezoelectric, optical, conductive and resistive types of transducer are presented. Where available, the current clinical status is specified.     

基于PDA的嵌入式ECG监护分析仪

目的为了方便快捷地实时监测心律失常,设计一种基于PDA的嵌入式ECG监护分析仪。方法仪器功能包括低功耗MSP430系列单片机控制心电信号的采集、放大、滤波、转换和在PDA上实时显示、存储和分析,并实现心电数据的远程传输。对仪器工作原理进行分析,把监护分析仪总体结构划分为子模块,并具体介绍各子模块的功能与实现。结果与结论与医院现有的三导全自动心电图机、Holter动态心电图机进行对照,本监护分析仪携带方便、操作简单、接收的心电信号无失真,连续工作时间大于20h等。各高频干扰信号对ECG信号无影响;软件可对心电信号进行实时显示,可连续记录15h的原始心电数据。     

多发性骨髓瘤骨髓活检与形态学检查的比较(附31例分析)

目的 :探讨多发性骨髓瘤 (MM)骨髓涂片与活检联合应用在 MM诊断和治疗中的价值。方法 :31例 MM骨髓涂片采用瑞氏染色 ,骨髓活检采用塑料包埋法薄切片经 HGF染色。结果 :骨髓活检与骨髓涂片增生程度完全相符者占 48% ,活检高于形态者占 39% ,形态高于活检者占 13%。瘤细胞检出例数活检与形态完全相符者占 39% ,活检高于形态者占 48% ,形态高于活检者占 13%。结论 :骨髓涂片与活检结合可相互补充 ,对 MM诊断和治疗有十分重要的价值     

双途径化疗治疗晚期肺癌并胸腔积液33例报道

目的观察双途径化疗对晚期非小细胞肺癌合并胸腔积液的疗效.方法采用胸腔穿刺、CVP管植入灌注顺铂＋地塞米松与长春瑞宾（NVB）＋顺铂（DDP）联合静脉化疗相结合的方法治疗33例肺癌.结果治疗胸腔积液的有效率为93.94%,肺部病灶治疗有效率为48.48%.结论双途径给药为临床上值得推广的方法.     

修复贝尔克血透机同一报警编码不同故障原因的故障

重庆市中医研究院医疗器械维修小组在维修贝尔克(Bellco)公司的formula(tm)型血液透析机压力传感器的不同故障中的心得体会.     

腹腔镜阑尾切除术残端包埋技巧及临床应用

目的探讨腹腔镜阑尾切除术（LA）残端包埋技巧及临床应用。方法自2008年1月至2011年2月,行LA86例,采取四针正向浆肌层缝合包埋阑尾残端。结果 86例LA均顺利完成阑尾残端包埋,手术时间22～70min,平均36min,术后住院时间2～5d,平均2.8d,无并发症发生。随访71例,随访时间3～12个月,无腹痛症状及其他并发症发生。结论腹腔镜下四针正向缝合包埋阑尾残端,符合缝合习惯,操作简单,安全可行,容易在临床开展应用。     

Facts and Fallacies About Measuring Blood Pressure in Rats

Although blood pressure can easily be measured in anesthetized rats by simply connecting a catheter to a pressure transducer, repeated measurements taken over long periods of time in awake rats are much more difficult to make. For chronic experiments two methods are now commonly used: direct recording from chronically-implanted arterial catheters, or indirect measurement with the tail-cuff method. Direct recording of intraarterial pressure can be done continuously and is more accurate, but technically more demanding. On the other hand, although tail-cuff measurements are less accurate, they do not require surgery and can be repeated almost indefinitely. With most tail-cuff methods the rats are preheated to dilate the tail vessels and thereby facilitate pulse detection, but with the new IITC photoelectric sensor indirect measurements of systolic as well as of mean arterial pressure can be made without external preheating. Even with a properly validated tail-cuff method, however, errors can still occur particularly when it is used to quantify modest blood pressure changes like those during development of hypertension, or following administration of vasoactive drugs. To safeguard against such errors, each laboratory should always validate its own tail-cuff method under uniform experimental conditions similar to those existing when the method is actually used. Additionally, all blood pressure differences thereby detected should be verified by direct measurement of intraarterial pressure in the same rats.