阴道毛滴虫与沙眼衣原体共生关系的初步探讨

目的 :为了探讨阴道毛滴虫与沙眼衣原体共生、协同致病的关系 ,对南通市公安局收容教育所非淋菌性阴道炎、尿道炎 (后称非淋患者 )患者以及本市某国营纺织厂同龄纺织女工阴道毛滴虫的寄生情况进行了 6个月以上的连续性观察。方法 :对 2 0 0 1年到 2 0 0 3年期间南通市公安局收容教育所非淋患者 1983例女性和随机抽样国营纺织厂纺织女工 2 84人分别采用生理盐水涂片法镜检阴道毛滴虫滋养体。结果 :非淋患者阴道毛滴虫滋养体的平均阳性检出率为 2 0 .6 8% (41/1983) ,明显高于正常对照组 (1.0 6 % ,3/2 84 ) ,差异显著 (X2 =2 5 .2 9,P <0 .0 0 1) .其中沙眼衣原体感染者 ,阴道毛滴虫滋养体的阳性检出率有增高趋势。结论 :非淋患者中沙眼衣原体感染时 ,其阴道毛滴虫阳性检出率高于其他患者。同时选择部分病例采用单纯抗非淋病治疗和抗非淋病与抗滴虫同时治疗进行比较并跟踪追访 6个月 ,缓解和消除症状后者明显优于前者 ,少数不规范治疗或擅自放弃治疗的人 ,延误病情或使病程迁延而转为慢性 ,给根治来一定的难度。建议注意阴道毛滴虫与沙眼衣原体共同感染协同致病的情况 ,要鉴别病原体 ,联合用药 ,提高疗效。     

性罪错人群阴道毛滴虫寄生情况的连续观察

目的:为了解阴道毛滴虫在性罪错人群寄生的情况,探讨和分析阴道毛滴虫寄生与性罪错人群间可能存在的联系。方法:对南通市公安局收容教育所性罪错人群以及本市某国营纺织厂健康纺织女工阴道毛滴虫的寄生情况进行了6个月～1年的连续性观察。对1998～2001年间南通市公安局收容教育所性罪错人群3490人和随机抽样国营纺织厂纺织女工284人分别采用生理盐水涂片法镜检阴道毛滴虫滋养体。结果:性罪错人群中淋病双球菌、解脲文原体、沙眼衣原体和梅毒螺旋体感染者阴道毛滴虫滋养体的平均阳性检出率为31.54％,明显高于正常对照组(2.46％,7/284),差异非常显著(x~2=29.97,P<0.005),并且淋病双球菌、解脲支原体、沙眼衣原体和梅毒螺旋体混合感染者,阴道毛滴虫滋养体的阳性检出率高于单纯淋病、解脲支原体感染、沙眼衣原体感染和梅毒螺旋体感染者。结论:建议性罪错人群在接受淋病双球菌、解脲支原体、沙眼衣原体、梅毒螺旋体时,甚至包括艾滋病毒等特异性检测的同时,必须常规检查阴道毛滴虫滋养体,尤其应当高度重视性传播疾病混合感染,务必选用抗性病和抗滴虫相结合的治疗方法,有利于患者迅速痊愈。     

Dual Targeting of Function–Structure for Effective Killing of Pathogenic Free-Living Amoebae

Here we discuss the potential value of targeting both the hardy structure of the cyst state of the parasite, in addition to the active trophozoite form, to provide target-directed inhibition as a viable drug strategy in the effective eradication of parasites.     

Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae

Background

Development of new natural agents for parasitic diseases treatment has unexpectedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomonas gallinae.

Methods

The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 µg ml⁻¹ against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was evaluated in vitro. Samples containing medium without chitosan were also assayed as controls.

Results

The mortality rates at 0, 3 and 6 h post treatment with all concentrations were significantly different from control group (P<0.05). Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 µg ml⁻¹ were 93%, 95% and 96.7%, respectively.

Conclusion

The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.     

弓形体抗原分型的研究

使用免疫印迹法和显凝试验均能测出弓形体各虫株之间有抗原差异。根据5株弓形体相互显凝试验结果,可分为4种抗原型,定为Ⅰ、Ⅱ、Ⅲ和Ⅳ型,这是弓形体抗原分型的首次报道,可能与地区和来源有关。     

隐孢子虫的超微结构及排囊的观察

本文通过动物感染观察隐孢子虫的排囊情况,发现多数为持续排囊,少数呈间歇性排囊,同时通过病理改变和超微结构的观察对此虫的致病机制和生活史做了探讨。     

贾第虫研究──Ⅰ．西蒙氏贾第虫（Giardia simoni）滋养体和包囊对实验动物的感染

作者从自然感染西蒙氏贾第虫（Ｇｉａｒｄｉａｓｉｍｏｎｉ）的金黄地鼠肠道内和粪便中分离、收集滋养体和包囊，分别经口感染大鼠、小鼠、豚鼠和兔子。结果表明兔子和脉鼠均不感染，大鼠、小鼠均不同程度地感染该虫，且４周龄大鼠比８周龄大鼠更易感（Ｐ＜０．０５），而在小鼠中成年鼠与老年鼠对西蒙氏贾第虫的敏感性没有差异（Ｐ＞０．０５）。滋养体在感染动物的肠道内主要分布于十二指肠前段，中前段和中段。同时观察到结肠和直肠内有包囊，这表明该虫在大鼠、小鼠体内完成了其生活史。作者还对感染前后的滋养体作了蛋白银和铁苏木精染色比较，二者在形态上完全相同。     

Performance of an enzyme-linked immunosorbent-based serological assay for Entamoeba histolytica: Comparison with an indirect immunofluorescence assay using stored frozen samples

IntroductionEntamoeba histolytica infections are increasingly diagnosed as sexually transmitted infections in Japan. However, the stool ova–parasite examination (O&P) test has been the only approved diagnostic method used in Japan since production of the indirect immunofluorescence assay (IFA) serum antibody test was discontinued at the end of 2017. Herein, we assessed whether an enzyme-linked immunosorbent assay (ELISA)-based serological test could substitute for IFA.MethodsThis cross-sectional study used stored frozen serum samples from the Biobank of the National Center for Global Health and Medicine. A serological ELISA-based test was performed on these samples and their titers were compared with those previously measured by IFA based on the medical record data.ResultsSixty seven stored frozen serum samples with differing recorded IFA antibody titers (16 samples with titers < ×100, 13 samples × 100, 16 samples × 200, 11 samples × 400, and 11 samples ≥ × 800) were analyzed. The sensitivity and specificity values for ELISA vs. IFA were 92.2% [95% confidential interval: 81.5–96.9] and 87.5% [64.0–97.8], respectively. A strong correlation between the antibody titers was confirmed by a one-way ANOVA (R square 0.83, p value < 0.0001) for the two diagnostic methods.ConclusionThe ELISA and IFA antibody titers for E. histolytica were well correlated, and results from these methods were highly concordant. Introduction of an ELISA-based serological test for E. histolytica should be considered to improve E. histolytica infection diagnosis in Japan.     

蓝氏贾第鞭毛虫滋养体细胞核的观察

①目的探究蓝氏贾第鞭毛虫滋养体的核结构。②方法用改良TYI-S-33培养基培养蓝氏贾第鞭毛虫滋养体,铁苏木素染色,光镜观察虫体的形态结构,透射电镜观察虫体的超微结构。③结果光镜观察可见1对椭圆形的泡状细胞核内各有1个细小的核仁,呈致密深染颗粒。透射电镜观察核膜完整为双层单位膜结构,核孔清晰,浅灰色的核质内有明显的圆形深染的核仁。④结论贾第虫滋养体已经形成了典型的细胞核和核仁。     

中药方剂治疗迈氏唇鞭毛虫感染1例

本文报道1例迈氏唇鞭毛虫感染者,并对其诊断和中医药治疗过程进行总结.