Expression of the c-erbB-2-encoded oncoprotein and progesterone receptor in human meningiomas

The present study investigated the expression of c-erbB-2 in 59 meningiomas, including different histological subtypes and anaplastic variants, by immunocytochemistry and molecular biological techniques. Immunohistochemistry using the monoclonal antibody FWP-51 directed against c-erbB-2-encoded oncoprotein gp185 demonstrated variable degrees of immunoreactivity in all meningiomas. The intensity of immunostaining correlated with the degree of expression as assessed by Western analysis in 28 meningiomas using polyclonal antiserum 21N. There was no correlation between the degree of expression and histological variants. Immunoreactivity of all menigiomas was distinctly less intense, however, than that of the human breast cancer cell line SK-BR-3, and slightly lower than that of brain metastases of breast and ovarian carcinomas that served as positive controls for both methods. By Southern analysis all meningiomas showed a single copy of the c-erbB-2 gene. Non-neoplastic arachnoid cap cells also exhibited c-erbB-2 expression and the degree of immunoreactivity was comparable with the majority of meningiomas. These data argue against an overexpression of c-erbB-2 in meningiomas, but rather indicate a cell-type-specific constitutive expression of the c-erbB-2 gene product in meningiomas and their putative progenitor cells. Since a subgroup of meningiomas is known to express progesterone receptors (PR), gp185 immunoreactivity was compared to the hormone receptor status using monoclonal antibody KD68. Fifty-six percent meningiomas showed PR immunoreactivity, but there was no statistically significant correlation with the degree of gp185 expression.This study was supported by a grant of the Tumorzentrum Heidelberg/Mannheim (M.K., No. 10028060)     

Cervical tissue uptake of all-trans-retinoic acid delivered via a collagen sponge-cervical cap delivery device in patients with cervical dysplasia

The present study was undertaken to evaluate the systemic absorption and cervical tissue uptake of all-transretinoic acid (TRA), delivered via a collagen spongecervical cap delivery device in patients with intraepithelial cervical dysplasia. Ten patients with histologically proven mild or moderate cervical dysplasia were included in this pharmacologic study. The two TRA concentrations (0.05% and 0.372%) selected for study represent the starting and maximally tolerated doses used in phase I clinical trial. All-trans-retinoic-11-³H acid (³H-TRA, 500 Ci) was used to facilitate cervical tissue uptake studies. Cervical biopsies and post-treatment blood samples were obtained from each patient after TRA exposure. The uptake of TRA into cervical tissues four hours after drug administration was significantly increased at the maximally tolerated TRA dose. There was a rapid decrease in cervical tissue concentration of TRA at the 0.372% dose between 4 and 24 h after drug exposure, suggesting a relatively short elimination half-life of TRA in cervical tissues. HPLC analysis of post-treatment blood samples indicate that there was no systemic absorption of TRA after local cervical administration.     

Non-adenomatous colorectal polyposis syndromes

Gastrointestinal polyps are common lesions that usually present singly or in small numbers. Although the term ‘multiple colorectal polyposis’ was originally applied to patients carrying at least 100 large intestinal adenomas, it has subsequently become broadened to include patients carrying multiple polyps regardless of their nature. Most of the non-adenomatous polyposis syndromes are hereditary. They can be classified according to the dominant type of polyp, their distribution in the gastrointestinal tract and their potential for the development of gastrointestinal cancers. This review summarises their main clinical, genetic and histopathological features.     

艾滋病合并马尔尼菲青霉病江西首例报告

目的 分析艾滋病并发马尔尼菲青霉病的发病情况、临床特征、诊断要点。方法 对艾滋病合并马尔尼菲青霉病患者的临床资料进行总结。结果 艾滋病并发马尔尼菲青霉病的患者临床症状呈现多样性，但以马尔尼菲青霉病的表现为主。结论 艾滋病且易并发马尔尼菲青霉病，应引起临床高度重视。     

可来福接头在深静脉置管中应用

目的 观察可来福接头在深静脉置管中的应用优势，方法 对260例深静脉置管成功的患者分别采用可来福接头及肝素帽封管，随机分为两组，160例采用可来福接头，100例采用肝素帽，、观察回血、堵管情况及留置时间。结果 可来福接头明显优于肝素帽。结论 可来福接头输液简便易行，节省时间，减少耗材。     

锐利器械不同保护法的效果研究

目的:研究尖锐器械在低温灭菌过程中的保护方法。方法:将2012年9月~201 3年6月供应室清洗后的再生穿刺针类器械741个,随机分为头皮针软管组、尿管组,分别使用头皮针软管、尿管做成保护帽,对锐利器械采取保护措施,并与2010年1月~3月使用纱布保护套的335个尖锐器械作对比,对其保护效果进行研究。结果:头皮针软管组包装及器械完好性达100%;尿管组纸塑袋包装破损32个,破损率8.7%,10个器械发生损坏占2.7%;纱布组管纸塑袋破损71个,破损率21.2%,器械损坏29个,破损率8.66%;三组包装破损率比较P<0.05,头皮针软管组、尿管组器械破损率分别与纱布组比较P<0.05,差异均有统计学意义。结论:头皮针软管能保护尖锐器械及包装的完好性,可根据器械的规格型号及数量选取相应的保护措施,以达院感要求。     

清胃消渴胶囊的鉴别研究

目的：建立清胃消渴胶囊的鉴别方法。方法：采用了理化鉴别和薄层色谱法鉴别。结果：两种方法可检出该制剂12味组成药材中的黄连、麦冬、枸杞子和组方中的多糖成分。结论：实验方法操作简便,重现性好,结果准确。     

应用经阴道三维超声检查宫内节育器分析

目的探讨经阴道三维超声检查宫内节育器的临床应用价值。方法应用阴道三维超声检查宫内节育器152例,首先使用二维超声进行矢状切面及冠状切面的检查,然后开启三维功能进行子宫及宫内节育器观察范围的三维超声表面模式和透明模式取样。结果152例宫内节育器三维图像透明模式取样完整显示率100%(152/152),表面模式取样完整显示率96.05%(146/152),二维超声完整显示率42.76%(65/152)。结论三维超声透明模式和表面模式合并使用探测宫内节育器有助于宫内节育器异常的明确诊断。     

透明帽在消化内镜诊疗中的应用进展

随着消化内镜诊疗技术的快速发展,各种内镜治疗附件的应用越来越普遍。透明帽作为最广泛应用的治疗附件之一,在内镜诊断及治疗中发挥了重要的作用,其中包括透明帽在内镜下黏膜切除术、内镜黏膜下剥离术、内镜下静脉曲张结扎术及硬化剂注射术、内镜诊断及取出食管入口细小异物等中的应用。本文现将透明帽在内镜诊治中的应用作一概述。     

康惠尔透明贴治疗静脉炎效果观察

为了预防和治疗因静脉输液而致的静脉炎,将59例静脉炎患者随机分为观察组(29例)和对照组(30例).对照组行常规50%硫酸镁湿热敷处理,观察组用康惠尔透明贴粘贴于患处进行治疗.比较2组显效及痊愈时间,差异有显著性意义(p＜0.01).提示康惠尔透明贴治疗静脉炎比常规湿热敷效果好.