应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

莱姆病（Ｌｙｍｅｄｉｓｅａｓｅ）是最近十年来才被认识的一种蜱媒传染流行病，Ｌｙｍｅ病的血清学诊断方法有间接荧光免疫法（ＩＦＡ），酶免疫试验（）ＥＩＡ），免疫印迹法（ＷＢ），梅里埃生物公司（Ｂｉｏｍｅｒｉｅｕｘ）推出ＶＩＤＡＳ免疫诊断系统能够自动定性和半定量地检查Ｌｙｍｅ病的抗伯氏包柔螺旋体抗（抗ＢＢ抗体）。本方法结合了酶和荧光免疫两种方法。硷性磷酸酶标记二抗的底物是４ｍｅｔｈｙｌｕｍｂｅｌｌｉｆｅ     

D-二聚体在急性肺栓塞的临床诊断价值

目的 探讨D-二聚体（DD）在急性肺栓塞诊断中的临床价值.方法 采用自动免疫分析系统（VIDAS）快速定量检测DD法对89例急性肺栓塞患者血浆中纤维蛋白降解产物DD进行检测,并与40例健康体检者血浆DD水平对比.结果 肺栓塞组患者血浆DD为（1620.6±45.8）μg/L,〉500μg/L者89例,阳性率100%.对照组（315.3±24.0）μg/L.两组血浆DD平均值相比较有差异统计学意义（P〈0.01）.结论 VIDASDD法检测方法敏感性高,DD对排除肺栓塞诊断有一定帮助,可作为排除肺栓塞的首选试验,值得在临床诊断中推广使用.     

三种方法检测泌尿生殖道沙眼衣原体的比较

目的用三种方法检测泌尿生殖道沙眼衣原体,评价其方法的敏感性和特异性.方法采用C-C快速法、PCR和VIDAS CHL三种方法检测150例尿道或宫颈分泌物标本.结果在150例患者中,阳性患者43例,阳性率为28.67％,C-C快速法检测的敏感性与特异性为97.67％和100％,PCR检测的敏感性和特异性为100％和98.13％,VIDAS CHL检测的敏感性和特异性为97.67％和97.20％.结论三种方法检测泌尿生殖道沙眼衣原体的敏感性和特异性无显著性差异(P＞0.05).     

非淋菌性尿道炎病原体检测的方案探讨

目的　探讨非淋菌性尿道炎 (NGU)病原体的常规检测方法。方法　报道 46 1份NGU标本用全自动免疫诊断系统 (VIDAS)检测沙眼衣原体 (Chlamydiatrachomatic,CT)。用支原体检测试剂盒 (MycoplasmaIST)检测 10 3份标本中支原体 (UreaplasmaurealytiumUU和MycoplasmahominisMH)的结果。 结果　沙眼衣原体和支原体的检测阳性率分别为 2 0 4%和 34 0 %。支原体阳性检测率男女间有显著差异P <0 0 5。结果还表明CT和支原体感染患者集中于 35岁以下年龄段。支原体药敏试验结果表明 ,对其敏感率最高的药物依次是原始霉素、交沙霉素、强力霉素和四环素。 14份正常人群标本用上述方法均未查出阳性结果。结论　VIDAS和IST具有特异性好、阳性率高、操作方便的优点 ,且非批次性操作可满足门诊、急诊病人立等可取的要求 ,有利于性传播疾病的及时控制。     

Evaluation of two VIDAS ®prototypes for detecting anti-HEV IgG

BackgroundHigh performance anti-hepatitis E virus (HEV) IgG assays are crucial for epidemiology.ObjectiveTo evaluate the performance of 2 prototypes developed for the VIDAS^® automatic system for detecting anti-HEV IgG, one based on the ORF2 antigen (ORF2 prototype) and the other on the ORF2 and ORF3 antigens (ORF2/3 prototype), with reference to the Wantai anti-HEV IgG assay.Study designThe sensitivity of each assay was determined by testing 113 blood samples, 63 from immunocompetent and 50 from immunocompromised patients, with a proven HEV infection defined by detecting HEV RNA. Their specificity was assessed with 103 blood samples that the Wantai assay indicated was negative for anti-HEV IgM and IgG, and negative for HEV-RNA. Cross reactivity was assessed using samples that were positive for hepatitis A virus IgG (n = 16), hepatitis C virus antibodies (n = 15), hepatitis B virus antigen and anti-HBc antibodies (n = 16), rheumatoid factor (n = 14), and negative for anti-HEV IgG with the Wantai assay.ResultsThe sensitivities in immunocompetent patients were: 95.2% (ORF2), 96.8% (ORF2/3), and 93.6% (Wantai); in immunocompromised patients they were: 66% (ORF2), 72% (ORF2/3), and 68% (Wantai). Both VIDAS prototypes detected low concentrations of anti-HEV IgG. The overall specificity was 100% (ORF2 prototype) and 98.1% (ORF2/3 prototype). Both VIDAS prototypes cross-reacted in five samples (9.6%), mainly those containing HCV antibodies or rheumatoid factor.ConclusionBoth VIDAS^® prototypes performed very well and appear to be suitable for routine detection of anti-HEV IgG.     

VIDAS定量检测D-二聚体的性能评价

目的了解并评价VIDAS定量检测D-二聚体的主要性能特点。方法依据NCCLS文件标准，对法国生物梅里埃公司全自动免疫分析系统（VIDAS）的D-二聚体检测性能进行评价。结果高值质控物（Control 1）、低值质控物（Control 2）及混合血浆的批内精密度CV分别为3．11％，3．43％和6．17％；批间精密度吖分别为4．35％，4．10％和7．48％；总重复性为8．2％，回收率为102％，准确度为97％。此次评估线性范围为45～4641．50ng／ml，仪器在此范围内线性良好（r=0．999）；血红蛋白（0．3mmol／L），三酰甘油（2mmol／L），胆红素（125μmol／l），类风湿因子（200IU／ml）对检测结果的影响度均小于厂家给定的批内变异率（10％）；20例健康人血浆标本进行D-二聚体正常参考值验证，均符合原试剂说明书提供的参考值。结论VIDAS定量检测D-二聚体的准确度、精密度、线性均较好，无携带污染率，检测简便易行，自动化程度高，尤其适合紧急情况下的个别检测。     

VIDAS CHL检测泌尿生殖道沙眼衣原体感染

目的 探讨VIDAS CHL法用于性病患者尿道拭子标本以及男性首段尿（FCU）标本沙眼衣原体（CT)检测的可行性。方法 使用组织培养法（TC）、VIDAS CHL检测法和聚合酶链反应（PCR）平行检测男性和女性拭子标本中CT，以组织培养为金标准，对VIDAS CHL和PCR进行评价。结果 232例男性标本TC阳性49例；VIDAS CHL和PCR法用于男性尿道拭子标本的敏感性分别为95.9%、93.9%，特异性分别为95.6%、94．5％，差异无显著性；VIDAS CHL法用于男性FCU的敏感性和特异性分别为85．7％和96．7％，与拭子标本作自身配对比较，检测结果无差异。151例女性拭子标本，TC法阳性23例，VIDAS CHL和PCR法的敏感性分别为100％和95．7％，特异性分别为96.1%、93.8%，差异无显著性。结论 VIDAS CHL法用于性病患者男性和女性拭子标本的CT检测，具有很高的敏感性和特异性，用于男性患者FCU的CT检测也是可行的；对于CT阳性率较高的性病人群，VIDAS CHL可以不做阻抑证实试验。     

Quality of life,health status,and health service utilization related to a new measure of health literacy: FLIGHT/VIDAS

Objective

Researchers have identified significant limitations in some currently used measures of health literacy. The purpose of this paper is to present data on the relation of health-related quality of life, health status, and health service utilization to performance on a new measure of health literacy in a nonpatient population.

Methods

The new measure was administered to 475 English- and Spanish-speaking community-dwelling volunteers along with existing measures of health literacy and assessments of health-related quality of life, health status, and healthcare service utilization. Relations among measures were assessed via correlations and health status and utilization was tested across levels of health literacy using ANCOVA models.

Results

The new health literacy measure is significantly related to existing measures of health literacy as well as to participants’ health-related quality of life. Persons with lower levels of health literacy reported more health conditions, more frequent physical symptoms, and greater healthcare service utilization.

Conclusion

The new measure of health literacy is valid and shows relations to measures of conceptually related constructs such as quality of life and health behaviors.

Practice implications

FLIGHT/VIDAS may be useful to researchers and clinicians interested in a computer administered and scored measure of health literacy.     

Diagnosis of deep vein thrombosis,and prevention of deep vein thrombosis recurrence and the post-thrombotic syndrome in the primary care medicine setting anno 2014

The requirement for a safe diagnostic strategy of deep vein thrombosis (DVT) should be based on an overall objective post incidence of venous thromboembolism (VTE) of less than 1% during 3 mo follow-up. Compression ultrasonography (CUS) of the leg veins has a negative predictive value (NPV) of 97%-98% indicating the need of repeated CUS testing within one week. A negative ELISA VIDAS safely excludes DVT and VTE with a NPV between 99% and 100% at a low clinical score of zero. The combination of low clinical score and a less sensitive D-dimer test (Simplify) is not sensitive enough to exclude DVT and VTE in routine daily practice. From prospective clinical research studies it may be concluded that complete recanalization within 3 mo and no reflux is associated with a low or no risk of PTS obviating the need of MECS 6 mo after DVT. Partial and complete recanalization after 3 to more than 6 mo is usually complicated by reflux due to valve destruction and symptomatic PTS. Reflux seems to be a main determinant for PTS and DVT recurrence, the latter as a main contributing factor in worsening PTS. This hypothesis is supported by the relation between the persistent residual vein thrombosis (RVT = partial recanalization) and the risk of VTE recurrence in prospective studies. Absence of RVT at 3 mo post-DVT and no reflux is predicted to be associated with no recurrence of DVT (1.2%) during follow-up obviating the need of wearing medical elastic stockings and anticoagulation at 6 mo post-DVT. The presence or absence of RVT but with reflux at 3 to 6 mo post-DVT is associated with both symptomatic PTS and an increased risk of VTE recurrence in about one third in the post-DVT period after regular discontinuation of anticoagulant treatment. To test this hypothesis we designed a prospective DVT and postthrombotic syndrome (PTS) Bridging the Gap Study by addressing at least four unanswered questions in the treatment of DVT and PTS. Which DVT patient has a clear indication for long-term compression stocking therapy to prevent PTS after the initial anticoagulant treatment in the acute phase of DVT? Is 3 mo the appropriate point in time to determine candidates at risk to develop DVT recurrence and PTS? Which high risk symptomatic PTS patients need extended anticoagulant treatment?     

2种方法检测食品中沙门菌的实验研究

[目的]比较自动酶联荧光免疫分析系统(VIDAS)和常规细菌培养法(GB)检测食品中沙门菌的效果。[方法]2009年7月,使用VIDAS法和常规细菌培养法平行检测各种样品中沙门菌,以常规细菌培养法作参照,对VIDAS技术进行评价。[结果]160份样品中,VIDAS法检出15份阳性样品,阳性率为9.38%,分离出12株沙门菌株;常规细菌培养法检出12份阳性样品,阳性率为7.50%,分离出12株沙门菌株。[结论]VIDAS法敏感、特异、简便、快速,可作为食品中沙门菌的快速筛检,常规培养法主要作为沙门菌的确证试验。