Hearing in Noise Test Brazil: standardization for young adults with normal hearing

IntroductionIndividuals with the same ability of speech recognition in quiet can have extremely different results in noisy environments.ObjectiveTo standardize speech perception in adults with normal hearing in the free field using the Brazilian Hearing in Noise Test.MethodsContemporary, cross-sectional cohort study. 79 adults with normal hearing and without cognitive impairment participated in the study. Lists of Hearing in Noise Test sentences were randomly in quiet, noise front, noise right, and noise left.ResultsThere were no significant differences between right and left ears at all frequencies tested (paired t − 1 test). Nor were significant differences observed when comparing gender and interaction between these conditions. A difference was observed among the free field positions tested, except in the situations of noise right and noise left.ConclusionResults of speech perception in adults with normal hearing in the free field during different listening situations in noise indicated poorer performance during the condition with noise and speech in front, i.e., 0°/0°. The values found in the standardization of the Hearing in Noise Test free field can be used as a reference in the development of protocols for tests of speech perception in noise, and for monitoring individuals with hearing impairment.     

下肢复杂骨折交锁髓内钉治疗粉碎骨折螺钉辅助固定的意义

[目的]探索在交锁髓内钉治疗下肢复杂骨折中粉碎骨折块辅助固定的意义。[方法]下肢复杂骨折78例根据AO分型均为C型骨折，通过交锁髓内钉内固定，辅以拉力螺钉固定。[结果]术后摄片骨折及骨折块达解剖或功能复位，随诊6～24个月，优良率为94．88％（74／78例）。[结论]四肢复杂骨折治疗中，粉碎骨折块的螺钉辅助固定能够加强交锁髓内钉的稳定性，减少断钉、断棒现象，有利于骨折愈合。     

La «coxa pedis»

Résumé: La ?coxa pedis? correspond à l'?articulation talo-calcanéo-navicularis?. Structurée comme une énarthrose, il est possible de lui définir une épiphyse représentée par la tête et le col du talus et une cotyle structurée comme une cavité ostéo-fibro-cartilagineuse, à la constitution de laquelle concourent, comme éléments squelettiques, la surface articulaire postérieure du naviculaire et les surfaces articulaires des petites (sustentaculum tali) et grandes apophyses du calcanéus qui forment l'articulation sous-talienne antérieure; parfois divisées entre elles par un sillon, le plus souvent elles constituent une seule formation articulaire. La surface articulaire comprise entre le naviculaire, le sustentaculum tali et la grande apophyse du calcanéus est complétée par un fibro-cartilage gléno?dien renforcé superficiellement par le ligament calcanéo-naviculaire plantaire qui, prenant naissance à la base et sur le contour antéromédial du sustentaculum tali, s'insère distalement sur le tubercule et le bord inféro-postérieur du naviculaire. Ce ligament correspond au fond de la coxa pedis et sous-tend une véritable gléno?de, revêtue de cartilage, en relation articulaire avec le versant inféro-médial de la tête du talus compris entre les versants du naviculaire et du calcaneus. De plus, la présence de corpuscules proprioceptifs dans le ligament calcanéo-naviculaire plantaire fait penser à une fonction réceptrice cybernétique également de la coxa pedis. Aux stades foetaux précoces (16^e-17^e semaine), les articulations talo-naviculaire et sous-talienne antérieure sont différenciées dans une unique structure articulaire présentant des caractéristiques morphologiques d'énarthrose. Dans un sens plus ample, ?coxa pedis? peut définir la signification fonctionnelle particulière d'une structure qui, de par ses données anatomiques, évolutives et cliniques, peut être analogiquement comparée à l'articulation coxo-fémorale avec laquelle, ainsi qu'avec le genou, elle s'intègre fonctionnellement dans la structure plus complexe du membre inférieur. La différenciation énarthrosique proximale et distale au membre inférieur, avec le joint interposé représenté par le genou, est une prémisse biomécanique aux mécanismes de rotation (plan orthogonal aux axes segmentaires du membre) indispensables pour amorcer la stabilisation du membre dans la phase portante (cha?ne cinétique fermée); et, à la succession des mécanismes intercurrents dans le plan frontal (translation latérale de la charge lors du démarrage de la phase portante) et dans le plan sagittal (phase oscillante). Une référence particulière est faite à la pathologie gléno?dienne dégénérative et au syndrome de déstabilisation péritalienne. Récemment, une référence à la ?coxa pedis? (1999) a été faite dans l'édition mise à jour de l'Encyclopédie Médico-Chirurgicale rédigée par Biga, Moulies et Mabit.      

不同来源HSP70的氨基酸组成分析

使用ＤＥＡＥ－５２离子交换层析和ＡＴＰ琼脂糖亲和层析方法，从人体肿瘤细胞株，大鼠肝脏和心脏、家兔和小鼠肝脏组织中纯化了主要的热应激蛋白－ＨＳＰ７０，用氨基酸自动分析仪分析它的氨基酸组成。     

EFFECTS OF STROPHANTHIDIN ON THE SLOW INWARD CURRENT IN GUINEA-PIG ISOLATED VENTRICULAR MYOCYTES

1. The effect of strophanthidin on the slow inward current (Isi) and on contractile force were studied in guinea-pig isolated ventricular myocytes and intact papillary muscles, respectively. In myocytes, both low (10 nmol/L) and high (1-10 mumols/L) concentrations had small or no effects in either direction on Isi whereas norepinephrine (10-100 nmol/L) increased it. To determine whether the same results are obtained after decreasing or increasing intracellular calcium or sodium, the same concentrations of strophanthidin were tested in different procedures that are known to (i) increase [Ca]i and decrease [Na]i (high [Ca]o, 3.6-5.4 mmol/L; low [Na]o, 112 mmol/L; (ii) decrease [Ca]i and increase [Na]i (low [Ca]o, 0.45-1 mmol/L; Sr, 1 mmol/L; (iii) decrease [Ca]i and [Na]i (Cd, 0.1-0.2 mmol/L); and (iv) increase [Ca]i and [Na]i (veratridine, 0.2 mumol/L). High [Ca]o and veratridine increased whereas low [Ca]o and Cd decreased Isi. In contrast, during these various procedures, strophanthidin had small and inconsistent effects at a low or high concentration. In intact papillary muscles, low strophanthidin decreased whereas high strophanthidin increased contractile force. It is concluded that strophanthidin has little direct or indirect effect on Isi and that the decrease in force by low and increase in force by high concentrations in intact muscle are probably related to demonstrated decrease and increase, respectively, in intracellular sodium activity.     

Cell-mediated immunity in leprosy and transfer of delayed hypersensitivity reactions

Eighty-nine patients with leprosy, 65 classified as lepromatous and 24 as tuberculoid, were examined in this study. Skin test responses to protein antigens and dinitrochlorobenzene (DNCB) were depressed in lepromatous patients compared to controls. Tuberculoid patients did not exhibit a significant depression to microbial antigens, but they showed a definite depression in the ability to be sensitized with DNCB. The transfer of delayed hypersensitivity reactions to tuberculin, trichophytin, and lepromin (Fernandez and Mitsuda reactions) was accomplished in lepromatous and indeterminate leprosy patients using viable lymphocytes from donors presenting positive reactions to these antigens. The lepromin reaction was also transferred to patients with South American blastomycosis and cutaneous leishmaniasis. The positive reactions of adoptive immunity were confirmed by histologic examination of skin biopsies.     

Grading score system: A method for evaluation of the degree of senescence in Senescence Accelerated Mouse (SAM)

For evaluation of the degree of senescence in SAM-P, accelerated senescence prone mouse, formerly called SAM or prone series or P-series, consisting of SAM-P/1, SAM-P/2, SAM-P/3 and SAM-P/4 corresponding to P-1, P-2, P-3 and P-4 series, respectively, in the previous reports, and in SAM-R, accelerated senescence resistant mouse, formerly called resistant series or R-series, consisting of SAM-R/1, SAM-R/2 and SAM-R/3 corresponding to R-1, R-2 and R-3 series, respectively, in the previous reports, the grading score system was adopted. The items to be examined in this system include 11 categories selected from the clinical signs and gross lesions considered to be associated with the aging process. The degree of the senescence in each category was graded from 0 to 4 according to the detailed criteria devised in our laboratory. After 8 months of age each mouse was examined every 4 months, and some of the mice were examined after 2 months of age.In almost all categories, the grading score and incidence began to increase from 4 or 6 months of age and continued to increase with advancing age in both SAM-P and SAM-R. The increase, however, was more marked in SAM-P than in SAM-R. The slow but steady increase in the SAM-R levelled out at 24 months of age and was comparable to that of 12 months of age in SAM-P. In both SAM-P/1 at 8 months of age and SAM-R/2 at 12 months of age, there was a significant reverse correlation between total score of this grading score system and length of residual life after examination.Systematic and extensive studies using the grading score system showed that if the validity of the system is, based on “irreversibility” and “universality” of the changes in     

Effect of o,p-dichlorodiphenyldichloroethane and perthane in vitro on glutathione reductase activity in the adrenals of dogs and guinea pigs

o,p-Dichlorodiphenyldichloroethane (o,p-DDD) and Perthane, when added in a concentration of 312 M to homogenate and cytoplasmic fraction of dog adrenals, activate glutathione reductase. In a concentration of 156 M, o,p-DDD and Perthane do not affect glutathione reductase activity of the dog adrenals. When given in vitro, o,p-DDD and Perthane activate glutathione reductase of the guinea pig adrenals. o,p-DDD has no effect on glutathione reductase activity of the cytoplasmic fraction of dog liver and kidney, thus confirming the high specificity of its effect on the adrenal cortex.Laboratory of Pathophysiology, Institute of Endocrinology and Metabolism, Kiev. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 85, No. 2, pp. 159–161, February, 1978.     

苯并（a）芘代谢产物诱发BALB／3T3细胞程序外DNA合成（UDS）的研究

本实验采用单标记和双标记法,分别检测4种苯并(a)芘代谢产物(anti-BPDE,syn-BPDE,3-OH-BP和9-OH-BP)对BALB/3T3细胞DNA合成和程序外DNA合成(UDS)的影响,结果表明,anti-BPDE、syn-BPDE、3-OH-BP和9-OH-BP均在不同程度上使BALB/3T3细胞DNA合成增加,但只有anti-BPDE、3-OH-BP和9-OH-BP可诱发BALB/3T3细胞的UDS,说明这些苯并(a)芘代谢产物可损伤BALB/3T3细胞的DNA,同时,这种效应与苯并(a)芘代谢产物的立体结构有关。     

Pattern of mtDNA Variation in Three Populations from São Tomé e Príncipe

We have analysed the matrilineal genetic composition of three self‐reported ethnic groups from São Tomé e Príncipe (Gulf of Guinea), an African archipelago whose settlement begun in the late fifteenth century. Sequence data from the hypervariable segments I (HVS‐I) and II (HVS‐II) were obtained for 30 Angolares, 35 Forros and 38 Tongas. The repertory of mtDNA lineages in São Tomé e Príncipe denoted a fully African maternal pool, primarily arisen from a Central/Southwestern substratum. The absence of any lineages of putative European descent means that the European impact at the mitochondrial pool was virtually nil. Angolares showed a clear reduction of mtDNA diversity and a slight genetic differentiation relative to Tongas or Forros, whereas the latter two groups did not present any signs of genetic boundaries between each other. The data obtained here reinforce the depiction of genetic substructuring in São Tomé e Príncipe previously derived from Y‐chromosome STRs. In addition, the crossing of mtDNA and Y‐STR information led to the inference that the female mediated gene flow within the archipelago was less restricted than the male, a pattern that could be framed in the cultural traditions and socio‐historical interactions among the groups.