排序方式: 共有5条查询结果,搜索用时 9 毫秒
1
1.
Saki Maeda-Otsuka Ikko Kajihara Yukino Tasaki Saori Yamada-Kanazawa Ryoko Sakamoto Soichiro Sawamura Mamiko Masuzawa Mikio Masuzawa Yasuyuki Amoh Daichi Hoshina Riichiro Abe Yoshihiro Komohara Hironobu Ihn 《Journal of dermatological science》2019,93(2):123-132
Background
Angiosarcoma is a rare malignant tumor with a poor prognosis. It is known that hypoxic condition activates tumor progression in several cancers. Additionally, hypoxic tumor microenvironment accelerates immune escape. However, the presence and significance of hypoxia in angiosarcoma has not been adequately investigated.Objective
To study the role of hypoxia in the progression of angiosarcoma.Methods
The protein level of hypoxia inducible factor-1α (HIF-1α) in angiosarcoma was examined using immunohistochemistry and immunoblotting. To study the effect of hypoxia on tumor progression, cell proliferation, migration, invasion, and tube formation assays were performed in angiosarcoma cells. The influence of tumor cell supernatant in hypoxia from angiosarcoma cells on immune escape and angiogenesis was analysed to investigate the modulatory effect of hypoxia on tumor microenvironment of angiosarcoma. The molecular mechanism related to these results was investigated using immunoblotting and real time RT-PCR.Results
HIF-1α protein was over-expressed in angiosarcoma tissues and cell lines under hypoxic conditions, and there was heterogeneity of oxygen supply in angiosarcoma. Hypoxia enhanced the proliferation, migration, and invasion abilities and inhibited tube formation in angiosarcoma cells. Tumor cell supernatant in hypoxia from angiosarcoma cells activated the monocyte invasion ability, facilitated its differentiation into M2-like macrophages, and suppressed cell-adhesion. These in vitro results were compatible to the pathological findings of angiosarcoma patients.Conclusion
Hypoxia plays a major role in progression of angiosarcoma cells by enhancing cell proliferation, migration, and invasion and by modulating the tumor microenvironment. 相似文献2.
Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment
下载免费PDF全文
![点击此处可从《Lasers in surgery and medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
3.
4.
花旗松素在Caco-2细胞模型中吸收转运机制及其抗炎活性研究 总被引:1,自引:0,他引:1
目的制备花旗松素单体并测定花旗松素在不同介质中的表观油水分配系数,研究其在结肠癌Caco-2细胞模型中吸收转运机制。方法酶解法制备花旗松素,采用高效液相色谱法测定花旗松素在37℃、不同pH值缓冲盐溶液和水中的饱和溶解度,通过油水分配系数计算公式得花旗松素表观油水分配系数;CCK-8实验考察花旗松素在Caco-2细胞中的安全浓度范围,再利用Caco-2细胞单层模型研究其双侧跨膜吸收转运机制;CCK-8实验考察花旗松素在人脐血管内皮细胞HDMEC细胞中的安全浓度范围,采用脂多糖诱导的HDMEC细胞建立炎症模型,用花旗松素进行干预,乳酸脱氢酶(LDH)试剂盒检测LDH的活力。结果花旗松素在不同溶剂中的lgP分别为0.29(0.1 mol/L盐酸)、0.48(pH 2.0)、0.46(pH 5.8)、0.34(pH 6.8)、0.26(pH 7.4)、0.38(水);花旗松素质量浓度在50~500μg/mL对Caco-2细胞无明显毒性作用;不同质量浓度花旗松素在Caco-2单层细胞模型中的转运具有时间依赖性,不同质量浓度花旗松素双侧转运Papp值无显著性差异,且均小于1×... 相似文献
5.
Endostatin inhibits angiogenesis by stabilization of newly formed endothelial tubes 总被引:14,自引:0,他引:14
Ergün S Kilic N Wurmbach JH Ebrahimnejad A Fernando M Sevinc S Kilic E Chalajour F Fiedler W Lauke H Lamszus K Hammerer P Weil J Herbst H Folkman J 《Angiogenesis》2001,4(3):193-206
Endostatin decreased vascular endothelial growth factor (VEGF)-induced formation of endothelial tubes and microvessels sprouting
from aortic rings and blocked their network. After cessation of treatment, the survival time of endostatin plus VEGF-treated
tubes was approximately doubled in comparison to VEGF alone. Endostatin antibody blocked VEGF-induced endothelial tube formation
and disrupted existing tubes. Endostatin immunostaining was localized between endothelium and basement membrane and in inter-endothelial
junctions of new, but not of quiescent, blood vessels. In tumors grown in SCID mice, endostatin immunostaining was stronger
accompanying blood vessel maturation and was significantly prominent in vessels of tumor marginal zone where angiogenesis
is highly active. These data indicate a new antiangiogenic action of endostatin stabilizing and maturating endothelial tubes
of newly formed blood vessels. Thus, strategies accelerating vascular stabilization and maturation could be promising in tumor
therapy.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
1