杭州地区急性呼吸道感染患儿人博卡病毒的检测及分析

目的了解杭州地区人博卡病毒(HBoV)的流行情况和基因变异情况。方法对杭州地区160例儿童急性呼吸道感染患者咽拭子样本进行HBoV检测,并对阳性样本进行常见呼吸道病毒的检测,最终选取HBoV单一阳性的HZ1株和HZ2株进行VP1基因扩增,将获得的序列上传Genbank并与其他国家和地区的8株HBoV的VP1基因进行序列比对和分析。结果杭州地区HBoV的检出率为6.9%(11/160),与其他常见呼吸道病毒的合并感染率达到45.5%(5/11),HZ1株和HZ2株与其他国家和地区的8株HBoV的VP1基因相似性达到99%以上。结论研究证明杭州地区急性呼吸道感染患儿中存在HBoV的感染,与其他呼吸道病毒有较高的合并感染,从分离到的HZ1株和HZ2株来看,杭州地区流行的是ST2基因型,其外壳蛋白VP1基因变异很小。     

人类博卡病毒致成人急性呼吸道感染的临床研究

目的：探讨人类博卡病毒（HBoV）致成人急性呼吸道感染（ARI）的临床特征,分析成人病毒性肺炎与HBoV感染的相关性。方法收集2013年5月至2014年1月在北京中医药大学东方医院感染科发热门诊就诊的81例ARI患者的鼻咽拭子,应用多重RT-PCR及PCR技术进行HBoV、呼吸道合胞病毒（RSV）、腺病毒（ADV）、鼻病毒（RhV）、流感病毒（Flu）、副流感病毒（PIV）、人类偏肺病毒（hMPV）、人冠状病毒（HCoV）、肠道病毒等9种病毒及肺炎支原体、肺炎衣原体的核酸检测,分析HBoV致成人ARI的临床特点。结果 HBoV阳性14例（17.3％）,其中10例（71.4%）为肺炎,4例（28.6%）为上呼吸道感染。 HBoV单独感染10例,混合感染4例。 HBoV感染者咳嗽10例（71.4%）,咳嗽伴喘憋1例（7.1%）,咯痰6例（42.9%）,发热14例（100.0%）,肺部闻及湿性啰音者4例（28.6%）。外周血白细胞正常或降低者11例（78.6%）,升高者3例（21.4%）。10例肺炎患者胸部X线片表现为斑片状模糊影9例,大片致密影1例。单侧肺炎7例,双侧肺炎3例,病变均位于中下肺叶。重症肺炎1例,为HBoV混合感染ADV及肺炎支原体。结论 HBoV是引起成人ARI的病原体之一,感染部位以下呼吸道为主。     

广州地区儿童呼吸道感染人博卡病毒的分子流行病学研究

人博卡病毒(HBoV)属细小病毒,为瑞典学者Allander 等[1]2005年从小儿下呼吸道感染样本中分离到的一种新呼吸道病原.目前世界各地均有HBoV研究相关报告[2],说明该病毒是世界范围内存在的人类致病原.为了解广州地区HBoV的分子流行病学特点和临床特征,对638例患儿临床标本进行检测.结果报告如下.     

Evaluation of the Xpert® group B streptococcus real-time polymerase chain reaction assay compared to StrepB Carrot Broth™ for the rapid intrapartum detection of group B streptococcus colonization

Human bocavirus DNA was detected by means of a quantitative, real-time polymerase chain reaction at low levels in the 5.51% of sera obtained from healthy blood donors, suggesting that viral detection in blood is not necessarily associated with disease status.     

The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant Lesions

Background: Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorialsubject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV)as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it intissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genomeand its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue. Method:in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects hadhealthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics softwareCLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations. Result: a total of 157 subjects wereparticipated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched bythe cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66,respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencinganalysis showed all were HBoV-1 genotype. Conclusion: our study showed there are low rate of HBoV genome inIranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1according to phylogenetic analysis.     

Human Bocavirus infection, Canada

Human Bocavirus was detected in 18 (1.5%) of 1,209 respiratory specimens collected in 2003 and 2004 in Canada. The main symptoms of affected patients were cough (78%), fever (67%), and sore throat (44%). Nine patients were hospitalized; of these, 8 (89%) were <5 years of age.     

Human bocavirus in stool: A true pathogen or an innocent bystander?

Human bocavirus (HBoV) is a parvovirus that was discovered only a decade ago and currently includes four genotypes. HBoV-1 is predominantly found in the respiratory tract, whereas HBoV-2, HBoV-3, and HBoV-4 are mainly detected in stool. HBoV-1 is known to be associated with respiratory tract infections. In stool, the prevalence of HBoV (1–4) is similar between patients with gastro-intestinal symptoms and healthy controls in most studies. Furthermore, often other viruses are concurrently present. Both findings suggest that HBoV in stool is an innocent bystander rather than a true pathogen. Nevertheless, several gaps in knowledge on the role of HBoV in stool remain to be addressed. All studies were performed in primarily immunocompetent patients. The role of HBoV in immunocompromised patients remains unknown.     

人博卡病毒实时荧光定量PCR检测方法的建立

目的建立人博卡病毒(human Bocavirus,HBoV)的实时荧光定量PCR(real-time fluorescent quanti-tative PCR,FQ-PCR)检测方法,检测呼吸道感染患儿标本的HBoV。方法设计HBoV NP1基因的引物和Taqman探针,扩增NP1基因片段,并将其克隆到pGEM-T Easy载体上,构建质粒标准品,建立FQ-PCR检测方法,进行敏感性、特异性试验,检测195份临床标本。结果所建立的FQ-PCR方法对临床其他呼吸道病毒不出现特异性扩增曲线,特异性好,线性范围为10～108copies/μl。195份临床标本中HBoV阳性12份,阳性率为6.2%。结论成功建立了HBoV实时荧光定量PCR检测方法并检测临床标本,为人博卡病毒感染的临床治疗提供快速、可靠的诊断依据。