Plasma fibronectin in psoriatic arthritis subgroups

Summary Plasma from 38 patients suffering from one of the five broad clinical subgroups of Psoriatic Arthritis (PA) were studied for soluble plasma Fibronectin (pFn). The mean total concentration of pFn was 453.03g/ml ± 142.83 SD, with a significant statistical difference (p<0.01) versus a healthy control group matched with respect to sex and age. In order to evaluate the biological role that pFn might play in this pathological condition, observed concentrations were correlated with the degree and duration of the psoriasis and arthritis. In addition, pFn was correlated to some biohumoral parameters that are modified during inflammatory processes (ESR, CRP, sCu, sFe, Hb) and to uric acid levels. Tissue typing (HLA) was done where possible. From our observations, we suggest that pFn most likely is not an acute phase protein and rather than having specificity for a particular disease, might, in widespread and severe cases be,a general and useful marker of the connective-tissue organizing and repairing response, following its injury.     

Radioimmunoassay for rat galanin: immunochemical and chromatographic characterization of immunoreactivity in tissue extracts

Galanin is a regulatory peptide with wide distribution in the central and peripheral nervous system and with numerous biological effects. Several radioimmunoassays based on antisera raised against porcine galanin have been used to measure immunoreactivity in rat tissues. However, considerable lack of parallelism has been observed between the porcine standard and rat tissue extracts, which may decrease the reliability of the quantitative data. The purpose of the present study was therefore to raise antibodies against rat galanin and establish a competitive radioimmunoassay for rat galanin. Two antisera, RatGal4 and RatGal5, were characterized in detail. The homogeneity of the immunoreactive material from several tissues was also investigated with column chromatography. At reverse-phase high-pressure liquid chromatography more than 95% of the immunoreactive material from rat CNS eluted as a single peak in the position of synthetic rat galanin, whereas almost half of the immunoreactive material from the intestine eluted in positions different from the synthetic peptide. Extracts of rat brains as well as jejunum diluted in parallel with the standard curve for both antisera. We conclude that measurements of rat galanin based on these antisera are therefore more reliable than those based on antisera raised against porcine galanin.     

血清纤维蛋白原水平及白蛋白/球蛋白比值与直肠癌患者生存预后的关系

目的探讨血清纤维蛋白原水平及白蛋白/球蛋白比值对直肠癌患者生存预后的影响。方法选取我科2010年1月至2013年1月收治的直肠癌患者120例,检测术前患者的血清FIB、AGR水平,根据血清FIB、AGR水平,分为FIB正常组(48例)和FIB高水平组(72例)、AGR正常组(45例)和AGR低水平组(75例);分析术前血清FIB、AGR与直肠癌患者的临床病理学特征关系,判断血清FIB、AGR对直肠癌患者生存预后的影响。结果患者检测术前血清FIB高水平组值6.53±1.28 g/L高于FIB正常组3.12±0.61 g/L,差异具有统计学意义(P<0.05);血清AGR低水平组值1.23±0.08低于AGR正常组值2.12±0.19,差异具有统计学意义(P<0.05)。血清FIB、AGR水平与直肠癌患者的BMI、肿瘤直径、TNM分期和淋巴结转移有关(P<0.05)。单因素分析显示:肿瘤直径>2cm、TNMⅢ期、淋巴结转移、FIB升高和AGR降低是影响直肠癌患者的危险因素(P<0.05);多因素分析显示:淋巴结转移、FIB升高和AGR降低是影响直肠癌患者生存预后的独立危险因素(P<0.05)。结论血清FIB、AGR值正常患者生存预后优于血清FIB升高、AGR降低患者;血清FIB水平升高、AGR水平降低可作为影响直肠癌患者生存率的的独立危险因素。     

Absoluter TBG-Mangel (Athyropexinämie) und hypertrophische obstruktive Kardiomyopathie

Zusammenfassung Bei einem Patienten mit hereditärer hypertrophischer obstruktiver Kardiomyopathie (HOCM) fehlt das thyroxinbindende Globulin (Thyropexin) völlig. Obwohl der Thyroxinspiegel extrem niedrig ist und die Schilddrüse eine erhöhte Jodavidität aufweist, erscheint der Patient klinisch euthyreot. Ein ähnliches Syndrom hat Ingbar 1961 beschrieben.Bei einem Onkel des Patienten und bei zwei Neffen, von denen einer eine asymmetrische Septumhypertrophie (ASH) aufweist, besteht eine Athyropexinämie; bei der Mutter, bei einer Schwester und einer Kusine ist das Thyropexin vermindert. Die Mutter leidet an einer hypertrophischen Kardiomyopathie (HCM).Herrn Prof. Dr. F. Heni zum 70. Geburtstag     

两种免疫抑制疗法治疗重型再生障碍性贫血比较研究

目的 :探讨重型再生障碍性贫血的适宜治疗方案。方法 :回顾性比较猪 -抗胸腺细胞球蛋白( P- ATG)单用 ( IST方案 )与联合方案兔 -抗人 T淋巴细胞球蛋白 ( R- ATL G)、环孢霉素 A( Cs A)、重组人粒 -巨噬细胞集落刺激因子 ( rhu GM- G- CSF) /重组人粒细胞集落刺激因子 ( rhu- G-CSF)及重组人红细胞生成素 ( rhu EPO) ( IST方案 )治疗 SAA疗效。结果 :IST方案 组不仅疗效高于 IST方案 组 ,且其有效者造血功能恢复更为迅速及良好。结论 :SAA患者更适宜于 IST方案 。     

ATG联合环孢素A治疗重型再生障碍性贫血的不良反应及其防治对策

目的观察抗胸腺细胞免疫球蛋白（ATG）联合环孢素A治疗重型再生障碍性贫血（SAA）的毒副作用、并发症,探讨其防治对策。方法对2003年1月至2008年7月于采用ATG联合CsA治疗的32例SAA患者的临床资料进行分析。结果32例患者均完成联合免疫抑制治疗,所有患者外周血淋巴细胞计数、血小板计数均于ATG治疗后迅速下降,出现过敏反应11例（34．4％）、血清病9例（28．1％）、肝功能异常21例（65．6％）,并有1例出现远期并发症,为骨髓增生异常综合征（MDS）样病态造血。结论ATG联合CsA治疗仍是SAA患者安全、有效的治疗措施,血清病、过敏反应、肝功能损害是其的主要不良反应,但及时给予激素、抗过敏及积极支持治疗可有效控制。     

小儿原发性肾病综合征并发感染临床分析

目的探讨小儿原发性肾病综合征并发感染的发生率及相关因素。方法回顾分析我院儿科住院的150例原发性肾病综合征患儿中发生感染的临床资料。结果150例中发生院内感染48例，发生院外感染25例，感染率为48．7％，其中呼吸道感染占多数；住院时间超过20d，患儿院内感染的发生率达79．4％，感染与血浆免疫球蛋白（IgG）、白蛋白下降有关。结论肾病综合征患儿易合并院内外感染，感染与住院时间长短、血浆免疫球蛋白以及白蛋白水平有关，缩短住院时间、提高免疫球蛋白、白蛋白水平可减少感染的发生。     

Management of Hepatitis B Virus Infection in Liver Transplantation Setting; The Rising Concerns and Growing Hopes,Report From 10th Congress of the Iranian Society for Organ Transplantation, 2011, Shiraz,Iran

Hepatitis B infection is the main cause of liver related mortality in many countries including Iran. Liver transplantation in cirrhosis due to HBV infection before 1990 was an absolute contraindication. Recurrent infection was a significant event in post liver transplant setting and resulted in increased risk of graft failure and death except successful transplanted individuals. Advances in antiviral prophylaxis have now made graft reinfection majority patients as a rare event. Graft and patient survival have been improved significantly during the past two decades, and consequences of transplantation for hepatitis B virus are now superior to those achieved for most other indications. This has encouraged many centers including the major liver transplantation center of Iran, in Shiraz, to provide liver transplantation to more patients with HBV related end stage liver disease. Management of these patients begins before transplantation along with special care after transplantation. There are some myths and doubts in the management of these patients and one should always balance the cost and efficiency. One of the major concerns is the high economic and social cost of recurrence and all possible efforts should be performed to avoid the ominous consequences of reinfection. Having a clear scientific grasp on the management of HBV cirrhosis before and after liver transplantation, options and protocols, and changing the concept which HBV infected are contraindicated ones for liver transplantation, and future hopes in increasing patients survival after liver transplantation using the new nucleosides analogues and availability of hepatitis B immunoglobulin in the transplantation setting. This scientific report paper outlines the insights communicated at the HBV and liver transplantation symposium during 10th Congress of the Iranian Society for Organ Transplantation, May 2011, Shiraz, Iran.     

丙种球蛋白联合更昔洛韦对呼吸道合胞病毒肺炎患儿肺功能影响

目的：探讨丙种球蛋白联合更昔洛韦对呼吸道合胞病毒（RSV）肺炎患儿肺功能的影响。方法：将87例RSV肺炎患儿随机分为两组,对照组43例给予更昔洛韦治疗,5mg·kg-1次-1,2次／d;观察组44例在对照组治疗基础上,加用丙种球蛋白治疗,400mg·kg-1次-1,1次／d,持续治疗14d;检测治疗前、后呼吸频率（RR）、潮气量（VT／kg）、达峰容积比（VPTEF／VE）、达峰时间比（TPTEF／TE）等肺功能指标变化。结果：观察组治疗总有效率高于对照组,差异具有统计学意义（P〈0．05）;治疗后观察组RR低于对照组,差异具有统计学意义（P〈0．05）;治疗后观察组TPTEF／TE及VPTEF／VE高于对照组,差异具有统计学意义（P〈0．05）。结论：更昔洛韦可有效抗病毒,丙种球蛋白可增强RSV肺炎患儿免疫力,促进炎症消除,两者合用可改善患儿肺功能,值得临床推广应用。     

Silk-elastinlike protein polymers for matrix-mediated cancer gene therapy

Silk-elastinlike protein polymers (SELPs) are recombinant polymers designed from silk fibroin and mammalian elastin amino acid repeats. These are versatile materials that have been examined as controlled release systems for intratumoral gene delivery. SELP hydrogels comprise monodisperse and tunable polymers that have the capability to control and localize the release and expression of plasmid DNA and viruses. This article reviews recent developments in the synthesis and characterization of SELP hydrogels and their use for matrix-mediated gene delivery.