全文获取类型
收费全文 | 1546篇 |
免费 | 202篇 |
国内免费 | 88篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 24篇 |
妇产科学 | 35篇 |
基础医学 | 224篇 |
口腔科学 | 15篇 |
临床医学 | 148篇 |
内科学 | 335篇 |
皮肤病学 | 15篇 |
神经病学 | 49篇 |
特种医学 | 30篇 |
外国民族医学 | 3篇 |
外科学 | 147篇 |
综合类 | 172篇 |
预防医学 | 67篇 |
眼科学 | 6篇 |
药学 | 77篇 |
中国医学 | 13篇 |
肿瘤学 | 467篇 |
出版年
2024年 | 3篇 |
2023年 | 45篇 |
2022年 | 95篇 |
2021年 | 136篇 |
2020年 | 103篇 |
2019年 | 99篇 |
2018年 | 94篇 |
2017年 | 94篇 |
2016年 | 92篇 |
2015年 | 104篇 |
2014年 | 140篇 |
2013年 | 100篇 |
2012年 | 91篇 |
2011年 | 97篇 |
2010年 | 56篇 |
2009年 | 55篇 |
2008年 | 53篇 |
2007年 | 47篇 |
2006年 | 39篇 |
2005年 | 31篇 |
2004年 | 18篇 |
2003年 | 12篇 |
2002年 | 28篇 |
2001年 | 7篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 8篇 |
1997年 | 13篇 |
1996年 | 23篇 |
1995年 | 10篇 |
1994年 | 17篇 |
1993年 | 15篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 8篇 |
1985年 | 9篇 |
1984年 | 5篇 |
1983年 | 1篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1972年 | 1篇 |
排序方式: 共有1836条查询结果,搜索用时 31 毫秒
1.
Xiying Fan Glen A. Bjerke Kent Riemondy Li Wang Rui Yi 《Molecular carcinogenesis》2019,58(12):2241-2253
MicroRNAs (miRNAs) play important roles in prostate cancer development. However, it remains unclear how individual miRNAs contribute to the initiation and progression of prostate cancer. Here we show that a basal layer‐enriched miRNA is required for prostate tumorigenesis. We identify miR‐205 as the most highly expressed miRNA and enriched in the basal cells of the prostate. Although miR‐205 is not required for normal prostate development and homeostasis, genetic deletion of miR‐205 in a Pten null tumor model significantly compromises tumor progression and does not promote metastasis. In Pten null basal cells, loss of miR‐205 attenuates pAkt levels and promotes cellular senescence. Furthermore, although overexpression of miR‐205 in prostate cancer cells with luminal phenotypes inhibits cell growth in both human and mouse, miR‐205 has a minimal effect on the growth of a normal human prostate cell line. Taken together, we have provided genetic evidence for a requirement of miR‐205 in the progression of Pten null‐induced prostate cancer. 相似文献
2.
Jinju Wang Shuzhen Chen Wenfeng Zhang Yanfang Chen Ji C. Bihl 《CNS Neuroscience & Therapeutics》2020,26(12):1255
AimsWe previously showed that the protective effects of endothelial progenitor cells (EPCs)‐released exosomes (EPC‐EXs) on endothelium in diabetes. However, whether EPC‐EXs are protective in diabetic ischemic stroke is unknown. Here, we investigated the effects of EPC‐EXs on diabetic stroke mice and tested whether miR‐126 enriched EPC‐EXs (EPC‐EXsmiR126) have enhanced efficacy.MethodsThe db/db mice subjected to ischemic stroke were intravenously administrated with EPC‐EXs 2 hours after ischemic stroke. The infarct volume, cerebral microvascular density (MVD), cerebral blood flow (CBF), neurological function, angiogenesis and neurogenesis, and levels of cleaved caspase‐3, miR‐126, and VEGFR2 were measured on day 2 and 14.ResultsWe found that (a) injected EPC‐EXs merged with brain endothelial cells, neurons, astrocytes, and microglia in the peri‐infarct area; (b) EPC‐EXsmiR126 were more effective than EPC‐EXs in decreasing infarct size and increasing CBF and MVD, and in promoting angiogenesis and neurogenesis as well as neurological functional recovery; (c) These effects were accompanied with downregulated cleaved caspase‐3 on day 2 and vascular endothelial growth factor receptor 2 (VEGFR2) upregulation till day 14.ConclusionOur results indicate that enrichment of miR126 enhanced the therapeutic efficacy of EPC‐EXs on diabetic ischemic stroke by attenuating acute injury and promoting neurological function recovery. 相似文献
3.
Pancreatic ductal adenocarcinoma (PDAC) is predicted to become the second leading cause of cancer-related mortality within the next decade, with limited effective treatment options and a dismal long-term prognosis for patients. Genomic profiling has not yet manifested clinical benefits for diagnosis, treatment or prognosis in PDAC, due to the lack of available tissues for sequencing and the confounding effects of low tumour cellularity in many biopsy specimens. Increasing focus is now turning to the use of minimally invasive liquid biopsies to enhance the characterisation of actionable PDAC tumour genomes. Circulating tumour DNA (ctDNA) is the most comprehensively studied liquid biopsy analyte in blood and can provide insight into the molecular profile and biological characteristics of individual PDAC tumours, in real-time and in advance of traditional imaging modalities. This can pave the way for identification of new therapeutic targets, novel risk variants and markers of tumour response, to supplement diagnostic screening and provide enhanced scrutiny in treatment stratification. In the roadmap towards the application of precision medicine for clinical management in PDAC, ctDNA analyses may serve a leading role in streamlining candidate biomarkers for clinical integration. In this review, we highlight recent developments in the use of ctDNA-based liquid biopsies for PDAC and provide new insights into the technical, analytical and biological challenges that must be overcome for this potential to be realised. 相似文献
4.
5.
Jin Wu Hao Wang Qing Li Qian-Ying Guo Si-Qi Tao Yu-Xian Shen Zheng-Sheng Wu 《Pathology, research and practice》2019,215(9):152523
Mammary carcinoma (MC) is one of most common malignancy in women, and ring finger protein 2 (RNF2) possesses various roles in vast human tumors. In MC tissues as well as in cell lines RNF2 exhibited high expression, had significant association with tumor size, lymph node status, TNM stage, patients’ poor survival, and promoted cell proliferation, colony formation, cell migration and invasion of MC cell lines which was mediated by downregulation of E-cadherin protein. These data reveal that RNF2 protein plays a vital role in the development of MC and may be a potential therapy target of MC. 相似文献
6.
用快速双抗体夹心ELISA检测囊虫病人血清中的循环抗原,其阳性率为67.5%;正常人血有的假阳性率为5.33%;包虫、肝吸虫和弓形虫病人血清的交叉反应率分别为6.9%、7.5%和12.5%。结果表明.该法与常规双抗体夹心ELISA相比具有较为敏感、快速、稳定和节省血清、试剂用量的优点,但其特异性有待进一步提高。用快速双抗体夹心ELISA和间接型ELISA检测第1至10疗程囊虫病人血清中的循环抗原和抗体,表明检测循环抗原考核囊虫病人的治疗效果优于检测抗体 相似文献
7.
microRNAs(miRNAs)是一类分布十分广泛的内源性非编码RNA,在动物、植物、病毒中广泛存在。miRNAs与肿瘤的发生发展预后有关,并在肿瘤的增殖、分化及调亡等方面有重要的作用。结合最近相关文献,本文就miRNAs与泌尿系肿瘤方面的进展作简要概述。 相似文献
8.
Peripheral blood stem cell autografting 总被引:1,自引:0,他引:1
Transplantation of haemopoietic stem cells provides a means whereby patients with malignant disease may be treated with increased doses of chemotherapy or chemoradiotherapy. Until recently, the bone marrow has been the sole source of these cells. However, haemopoietic progenitors can also be demonstrated in the blood and it has been known for more than twenty years that peripheral blood mononuclear cells are capable of repopulating the marrow in animals. This phenomenon has recently been reproduced in man. The use of peripheral blood rather than bone marrow for autologous stem cell rescue may have advantages in terms of ready access, availability in patients with compromised pelvic bone marrows, a lower risk of tumour contamination and more rapid granulocyte and immune recovery. However, clinical experience with peripheral blood stem cell autografting is still very small. This review discusses the characteristics of circulating stem cells, the methods by which they can be collected and stored and the information which has come from recent studies of their transplantation in man. 相似文献
9.
10.
布氏显微镜活血分析: 细胞流变学研究的新方法 总被引:14,自引:0,他引:14
布氏显微镜活血分析:细胞流变学研究的新方法骆秉铨*黄荣国*陈兴新*细胞流变学是研究血细胞流动变形的科学,在方法学上发展了一些比较成熟的方法,但多不能直接动态观察细胞水平的真实改变,有待完善和创新。我们采用布氏多功能显微镜的活血分析法[1],在高放大倍... 相似文献