消化道无线内窥镜便携式图像接收装置的硬件设计

消化道无线内窥镜便携式图像接收装置是在TMS320C6211DSP的基础上进行开发的。先对摄像胶囊发出的调制信号进行接收和解调，然后利用视频解码芯片SAA7114H的同步信号，经过时序逻辑变换，控制对断续视频信号的采集，并将其存储到CF卡中。此外．还利用高速DSP对图像信号进行预处理和压缩．可大大节约数据的存储空间和处理时间。     

miR-320c Regulates SERPINA1 Expression and Is Induced in Patients With Pulmonary Disease

IntroductionAlpha-1 antitrypsin deficiency (AATD) is a genetic condition resulting in lung and liver disease with a great clinical variability. MicroRNAs have been identified as disease modifiers; therefore miRNA deregulation could play an important role in disease heterogeneity. Members of miR-320 family are involved in regulating of multiple processes including inflammation, and have potential specific binding sites in the 3′UTR region of SERPINA1 gene. In this study we explore the involvement of miR-320c, a member of this family, in this disease.MethodsFirstly in vitro studies were carried out to demonstrate regulation of SERPINA1 gene by miR-320. Furthermore, the expression of miR-320c was analyzed in the blood of 98 individuals with different AAT serum levels by using quantitative PCR and expression was correlated to clinical parameters of the patients. Finally, HL60 cells were used to analyze induction of miR-320c in inflammatory conditions.ResultsOverexpression of miR-320 members in human HepG2 cells led to inhibition of SERPINA1 expression. Analysis of miR-320c expression in patient's samples revealed significantly increased expression of miR-320c in individuals with pulmonary disease. Additionally, HL60 cells treated with the pro-inflammatory factor lipopolysaccharide (LPS) showed increase in miR-320c expression, suggesting that miR-320c responds to inflammation.ConclusionOur findings demonstrate that miR-320c inhibits SERPINA1 expression in a hepatic cell line and its levels in blood are associated with lung disease in a cohort of patients with different AAT serum levels. These results suggest that miR-320c can play a role in AAT regulation and could be a biomarker of inflammatory processes in pulmonary diseases.     

短暂性脑缺血发作320排动态容积CT全脑灌注联合CT血管成像研究

目的:研究分析短暂性脑缺血发作320排动态容积CT全脑灌注联合CT血管成像。方法:在2018年10月-2019年10月于我院就诊的短暂性脑缺血发作患者中随机选择56例进行研究。所有患者均接受320排动态容积CT全脑灌注联合CT血管成像进行诊断。对比患者患侧与镜像对侧的血流量、血容量、平均通过时间、达峰时间以及延迟时间等灌注参数。结果:患侧与镜像对侧的血流量、血容量、平均通过时间、达峰时间以及延迟时间等灌注参数差异具有统计学意义(P<0.05),在平均通过时间、达峰时间和延迟时间的患侧与镜像对侧灌注参数比值方面,差异具有统计学意义(P<0.05)。结论:320排动态容积CT全脑灌注联合CT血管成像对于短暂性脑缺血发作进行诊断评价具有可行性,可有效评价患者的血管情况及脑组织血流灌注情况。     

Vitamin B12 and its binding proteins in hepatocellular carcinoma and chronic liver diseases

Abstract

Background. The vitamin B₁₂ (B₁₂)-binding protein haptocorrin (HC) has proven to be a potentially useful biomarker in patients with fibrolamellar hepatocellular carcinoma (HCC). Little is known concerning the level of HC and other B₁₂-related proteins in patients with HCC as compared to patients with other chronic liver diseases (CLDs) and healthy controls. We hypothesized that HC could be a biomarker of HCC. Aims. To investigate levels of HC and B₁₂-related proteins in HCC compared to CLDs and healthy controls. Methods. We investigated two patient populations: A cross-sectional cohort of HCC patients (n = 130), CLD patients (n = 102) and healthy controls (n = 46) and a cohort of 38 HCC patients studied at baseline and 1, 4, and 12 weeks following ablative treatment. Patients were evaluated by standard biochemistry, Child–Pugh-score and Barcelona Clinic Liver Cancer (BCLC) classification. We analyzed total B₁₂ by routine methods and HC, transcobalamin (TC), B₁₂ saturated TC (holoTC), and the soluble cell surface receptor for holoTC (sCD320) by in-house enzyme-linked immunosorbent assay. Results. HC showed higher median (range) levels for both HCC (590 [290–5860]) and CLD patients (620 [310–4010]) compared to controls (460 [250–2020]) (p < 0.01). Total B₁₂, TC, holoTC, and sCD320 showed elevated levels in both HCC and CLD compared to controls. Only holoTC changed following treatment, without a concurrent change in TC. Conclusion. B₁₂ and B₁₂-related proteins (total B₁₂, HC, TC, holoTC, and sCD320) show elevations in both HCC and CLD patients compared to controls, suggesting a relation to CLD in general rather than to primary liver cancer. Thus, HC is not useful as a biomarker for HCC.     

微小RNA-320a通过靶向FoxM1调控肝癌细胞迁移

目的：探讨微小RNA-320a (miR-320a)在肝癌细胞中对癌基因FoxM1的靶向调控作用以及对肝癌细胞迁移能力的影响,为肝癌治疗提供新的靶点。方法通过信息学工具网站http：//www.microRNA.org找到可能靶向调控FoxM1表达的微小RNA;利用蛋白印记和双荧光素酶报告基因试验验证miR-320a对FoxM1的调控作用;采用Transwell小室分析miR-320a和FoxM1对肝癌细胞迁移的影响。结果信息学分析表明,miR-320a在FoxM1的3'端非翻译区存在2个潜在结合位点。蛋白印迹实验显示miR-320a降低FoxM1在肝癌细胞中的蛋白水平,双荧光素酶报告基因试验显示miR-320a可以调控FoxM1表达。miR-320a类似物对FoxM1表达有下调作用（P<0.01）,miR-320a抑制物对FoxM1表达有上调作用（P<0.05）。Transwell试验表明miR-320a类似物可抑制肝癌细胞株Huh7、HCC-LM3的迁移能力,miR-320a抑制物可增加Huh7、HCC-LM3的迁移能力。当使用小干扰RNA下调FoxM1在这些细胞株中的表达后,miR-320a类似物和miR-320a抑制物对肝癌细胞迁移能力的调控作用明显减弱。结论 miR-320a有抑制肝癌细胞迁移的作用,这种抑制作用是通过靶向调控癌基因FoxM1来实现的。     

320排CTA在诊断及介入治疗颈部动脉夹层中的价值

目的：比较320排CT血管造影(CT angiography, CTA )和数字减影血管造影(Digital subtraction angiography, DSA)诊断颈部动脉夹层常见影像学征象的敏感性。方法：收集我院神经内科及放射介入收治的58例经DSA诊断为颈部动脉夹层的患者,回顾性分析并比较其320排CTA与DSA的影像学特征。结果：与传统的DSA检查结果相比,对照分析CTA诊断颈部动脉夹层的能力。结果发现CTA显示双腔征为67% (4/6),血管狭窄为94% (33/35),内膜瓣为85%(22/26),具有很高的敏感性;在显示血管闭塞及夹层动脉瘤方面二者无显著性差异(P>0.05)。结论：320排CTA作为一项有效、简便及非侵入性影像学诊断技术,可较清晰的显示颈部动脉夹层的多种影像学征象,对颈部动脉夹层临床诊治具有重要的指导意义。     

320排CT靶重建联合MRI扫描对孤立性肺结节中早期周围型肺癌的诊断价值

目的 探讨320排CT靶重建联合MRI扫描在孤立性肺结节良、恶性鉴别诊断及肺癌早期诊断中的价值。方法 回顾性分析延安市人民医院320排640层CT低剂量扫描、CT标准靶重建、联合1.5TMRI检查,对临床检出的42例肺内孤立结节(SPN)进行相关诊断研究,分析本项技术是否能够明显提高诊断的准确率。结果 42例可疑孤立性肺小结节中检出42例检出率为100%,常规CT扫描病变检出正确例数28例正确率为66.67%,靶重建后检出正确例数为34例准确率为80.95%,联合CT靶重建及MRI检出正确例数40例,准确率可高达95.42%。结论 CT靶重建技术联合MRI扫描简单易行,患者容易接受,且能提高诊断的准确性。