Laboratory preparation and evaluation of a multiparameter hemocytometry control

A protocol for the laboratory preparation of a multiparameter hemocytometry control is given. Human platelets, stabilized by a basically simplified and inexpensive fixation procedure, are added to our previously described white and red blood cell control. Evaluation of this multiparameter control shows good precision characteristics and acceptable mechanical stability for at least 7 weeks, as measured in the Coulter counter Model S Plus-II. The control can basically contribute to the realization of the essence of internal quality control: continuous self-auditing and continuous attempts at improvement of performance.     

血小板相关指标与肺结核诊断治疗的关联研究

目的 探讨肺结核患者的血小板参数,在诊断与治疗肺结核中的意义.方法 分别定量检测104例肺结核初治患者(无肺部感染49例,合并肺部感染55例)治疗前后和50例健康对照组的血小板分布宽度(PDW)、血小板平均体积(MPV)和血小板压积(PCT)等血小板指标.结果 PLT、MPV、PCT在无肺部感染组分别为237.25(99-579) 109/L、9.63(6.14-12.80)fL、0.229(0.094-0.590)％,在合并肺部感染组分别为270.64(106-525) 109/L、10.02(6.40-13.20)fL、0.272(0.119-0.540)％,健康对照组分别为197.39(101-326) 109/L、8.22(5.89-11.20)fL、0.160(0.059-0.360)％,前两组均较健康对照组显著增加(P＜0.05),且PLT、PCT合并肺部感染组较未感染组升高明显(P＜0.05);PDW三组间无明显变化.PLT、MPV、PCT的检测值在抗结核治疗后显著下降.结论 检测肺结核患者PLT、MPV和PCT可作为临床医生分析病情判断疗效的参考指标.     

Daily exposure to forty percent oxygen causes a decrease in platelet count

A study was undertaken to evaluate a previous observation made in this laboratory that hyperoxic treadmill training is associated with a decrease in the circulating platelet count (PLT). The subjects studied breathed air containing 40% oxygen for 30 min/day, 5 days/week, for 6 weeks, while at rest in the seated position. Such exposure resulted in a consistent fall in PLT between day 1 and day 5 of each week (P less than .01). In addition, there was a progressive decline in PLT over the 6 weeks of exposure to the hyperoxic air, PLT being inversely correlated with the duration of hyperoxic exposure (rs = -0.886, P less than .02). The total decrease in PLT over the 6 weeks was 56 +/- 46 x 10(9) l(-1) (P less than .025). Not surprisingly, these changes were mirrored in the plateletcrit (Pct). The decrease in PLT did not appear to be secondary to either hemodilution or reduced erythropoietic stimulation. The mechanism of production and the biological significance of these changes remain to be elucidated.     

The predictive value of mean platelet volume,plateletcrit and red cell distribution width in the differentiation of autoimmune gastritis patients with and without type I gastric carcinoid tumors

Abstract

Autoimmune gastritis is an autoimmune and inflammatory condition that may predispose to gastric carcinoid tumors or adenocarcinomas. The early diagnosis of these tumors is important in order to decrease morbidity and mortality. Platelet indices such as mean platelet volume and plateletcrit levels increase in inflammatory, infectious and malign conditions. The primary aim of this study was to explore wheter platelet indices and red cell distribution width have any predictive role in the discrimination of autoimmune gastritis patients with and without gastric carcinoid tumors. Also secondary aim of this study was to investigate whether any changes exist betwenn autoimmune gastritis and functional dyspepsia patients by means of platelet indices. Plateletcrit (0.22?±?0.06 vs. 0.20?±?0.03%, p?<?0.001) and red cell distribution width (16.11?±?3.04 vs. 13.41?±?0.95%, p?<?0.001) were significantly higher in autoimmune gastritis patients compared to control group. Receiver operating curve analysis suggested that optimum plateletcrit cut-off point was 0.20% (AUC: 0.646), and 13.95% as the cut off value for red cell distribution width (AUC: 0.860). Although plateletcrit (0.22?±?0.06 vs. 0.21?±?0.04%, p?=?0.220) and mean platelet volume (8.94?±?1.44 vs. 8.68?±?0.89?fl, p?=?0.265) were higher in autoimmune gastritis patients without carcinoid tumor compared to patients with carcinoid tumors, these parameters were not statistically significant. Changes in plateletcrit and red cell distribution width values may be used as a marker in the discrimination of autoimmune gastritis and fucntional dyspepsia patients but not useful in patients with gastric carcinoid tumor type I.     

血小板自动计数4种参数值及其临床意义

为了解血小板自动计数PLT、MPV、PCT和PDW4参数的临床意义,对2926例冠心病、高血压、糖尿病等17种常见病和662例手术、分娩及放射介入治疗前后患者进行了以上4种参数的测定,发现糖尿病、上消化道出血和自血病时MPV升高,贫血时降低。PCT在上消化道出血时升高,冠心病和肝硬化时降低。PDW 在冠心病和肝硬化时升高,而脑梗塞时则呈持续显著升高。此外,手术后MPV升高而分娩后PCT升高。结果提示,这些值的变化可作为疾病诊断和疗效观察的依据。PDW在脑溢血时下降和脑梗塞时增高,又可作为两者的鉴别指标。     

Evaluation of Platelet Indices in Lung Cancer Patients

Background: In this study, we aimed to determine platelet indices such as platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), Plateletcrit (PCT) platelet count (PLT) in lung cancer cases, and evaluate any relationships between these parameters and stage or histologic types. Materials and Methods: This retrospective study covered 44 lung cancer patients and 47 healthy subjects. Platelet indices including PLT, PCT, MPV, PDW were estimated and compared with normal subjects. The results were evaluated statistically. Results: The PDW value was significantly higher in the cancer group compared to the control group; however, the values for PCT and MPV were lower. Conclusions: We suggest potential use of platelet indices in diagnosis of lung cancer.     

A two-year dietary carcinogenicity study of cyadox in Sprague-Dawley rats

To investigate the potential carcinogenicity of cyadox, an antimicrobial agent, four groups of Sprague-Dawley rats (50 rats/sex/group) were fed diets containing cyadox (0, 200, 600 or 2000 mg/kg) for up to two years. There were significant decreases in body weight, feed intake and feed efficiency in both genders during most of the period in the 2000 mg/kg group. Significant decreases in serum ALT were observed in the 2000 mg/kg group at weeks 52, 78 and 104. For the control, 200, 600, and 2000 mg/kg groups, the tumor incidence in females was 33.3%, 37.2%, 40.0% and 19.0%, while it in males it was 18.9%, 2.6%, 17.1% and 13.6%, respectively. At histopathology, no increases in tumor incidence were attributed to treatment with cyadox. The mild swelling and fatty degeneration in hepatocytes, and mild swelling and tubular necrosis in the kidney were observed in 2000 mg/kg group. The no-observed-effect-level (NOEL) for carcinogenicity of cyadox fed to rats was 2000 mg/kg diet (132.18–156.28 mg/kg b.w./day). In conclusion, cyadox was not carcinogenic to rats with the liver and kidney as the target organs, and the side chain may be involved in toxicity and carcinogenicity mediated by QdNOs.     

Plateletcrit for predicting prognosis in patients with hepatic sinusoidal obstruction syndrome caused by pyrrolizidine alkaloid

BackgroundPlatelet index was reported to be used as a potential prognostic marker in patients with liver fibrosis. We aimed to explore the association between plateletcrit (PCT) and severity of hepatic sinusoidal obstruction syndrome (HSOS).MethodsSeventy consecutive patients who diagnosed as HSOS by CT and medical history during January 2017‐November 2021 were included. All patients were divided into two groups which confirmed as favorable prognosis and poor prognosis on the basis of Child‐Turcotte‐Pugh score system. The clinical manifestation and laboratory parameters of two groups were retrospectively selected. PCT was evaluated within two groups, and the diagnostic accuracy was evaluated by the area under the receiver operating characteristic curve.ResultsThe significant difference between the two groups not only in diarrhea, abdominal pain, abdominal distention, urine volume, and skin ecchymosis (p < 0.005), but also in WBC count, NE count, PLT count, TBIL, and D‐Dimer (p < 0.005) were found. The PCT level was significantly higher in HSOS patients with poor prognosis (0.169 ± 0.060) than favorable prognosis patients (0.110 ± 0.047). The area under the receiver operating characteristic curve of RDW in predicting poor prognosis was 0.781, with 67.70% sensitivity and 79.5%specificity.ConclusionsThe PCT level was correlated positively with the poor prognosis in HSOS patients. PCT can be a promising indicator for predicting prognosis in HSOS     

糖尿病患者血小板4项参数与血管病变探讨

目的探讨糖尿病患者血小板参数变化及其临床意义。方法使用XE-2100型全自动血细胞分析仪分别测定健康对照、糖尿病和冠心病患者血小板计数（PLT）、血小板平均体积（MPV）、血小板分布宽度（PDW）及血小板比容（PCT）。结果与对照组比较,2型糖尿病有血管病变组和冠心病组的PLT、MPV、PDW差异有统计学意义（P〈0．01）,PCT差异无统计学意义（P〉0．05）;2型糖尿病无血管病变组血小板4项参数差异无统计学意义（P〉0．05）。结论血小板参数的变化在糖尿病血管病变发生、发展中有重要作用,对临床研究糖尿病并发症有较大参考价值。