上腹部不适患儿幽门螺杆菌感染类型研究

目的了解以上腹部不适就诊的14岁以下患儿幽门螺杆菌（Hp）的感染情况，探讨应用免疫印迹法分型诊断儿童Hp感染的临床意义。方法采取705例以上腹部不适就诊患儿的静脉血。应用免疫印迹法进行Hp抗体谱带分析，分型诊断Hp感染。当检出有与Hp细胞毒相关蛋白A（CagA）和（或）空泡毒蛋白A（VacA）相关的抗128、116、95、和91kD蛋白抗体时，Hp的感染类型定为Ⅰ型；仅有与Hp尿素酶和鞭毛蛋白相关的抗30、66、50和54kD蛋白抗体时。Hp的感染类型定为Ⅱ型。结果共捡出Hp抗体阳性468例。总阳性率为66．38％，其中Ⅰ型Hp占44．87％，Ⅱ型Hp占55．13％。共有63例患儿经内镜检查明确诊断患有消化性溃疡，其中的Hp抗体总阳性率为84．54％。Ⅰ型Hp抗体总阳性率为82．54％。比未被诊断为消化性溃疡惠儿的64．50％和24．61％均显著增高。结论利用免疫印迹法可方便地对儿童Hp感染进行血清学分型诊断；Hp感染在腹部不适的患儿中较为普遍，尤其是在消化性溃疡患儿中；Ⅰ型Hp感染与消化溃疡的关系更为密切。应进行根除治疗。     

8号染色体三体对急性粒、单核细胞白血病细胞生物学和临床特征的影响

为了探讨8号染色体三体(8三体)对急性粒单、单核细胞白血病(M4、M5)细胞生物学及临床特征的影响,应用G显带或R显带技术及流式细胞仪对56例M4、M5患者进行核型及免疫表型检测,并对其临床特征进行回顾性分析。结果表明:56例患者中34例(60.7%)正常核型;10例(17.9%)8三体异常核型,其中3例(5.4%)为单纯8三体核型,余下12例(21.4%)为其他异常核型(不包括8三体)。8三体患者的年龄偏大,外周血原始 幼稚细胞百分比和外周血白细胞(WBC)计数均较低,无病生存期(DFS)较短(P均<0.05)。8三体患者CD34、CD117和CD56的表达频率较高,而CD11b、CD14和CD15则较低(P分别<0.01、0.05、0.05、0.01、0.05和0.005)。结论:8三体的M4、M5患者预后较差,8三体可能与单核细胞分化成熟受抑有关。     

白血病和淋巴瘤免疫分型的研究

应用单克隆抗体ABC法、PAP法、酶组化等多标记方法研究白血病和淋巴瘤。其中急性白血病3例、恶性淋巴瘤4例、骨髓瘤3例、反应性增生2例、转移癌1例。本组发现1例急性单核——淋巴细胞混合型白血病,1例急性Tr淋巴细胞白血病/淋巴瘤。本文还讨论了MCAb和多标记研究在白血病、淋巴瘤的免疫分型、鉴别诊断的意义。     

The expression of nestin delineates skeletal muscle differentiation in the developing rat esophagus

The muscularis externa of the developing rodent esophagus is initially composed of smooth muscle, and later replaced by skeletal muscle in a craniocaudal progression. There is growing evidence of distinct developmental origins for esophageal smooth and skeletal muscles. However, the identification of skeletal muscle progenitor cells is controversial, and the detailed cell lineage of their descendants remains elusive. In the current study, we carried out multiple labeling immunofluorescence microscopy of nestin and muscle type-specific markers to characterize the dynamic process of rat esophageal myogenesis. The results showed that nestin was transiently expressed in immature esophageal smooth muscle cells in early developing stages. After nestin was downregulated in smooth muscle cells, a distinct population of nestin-positive cells emerged as skeletal muscle precursors. They were mitotically active, and subsequently co-expressed MyoD, followed by the embryonic and later the fast type of skeletal muscle myosin heavy chain. Thus, the cell lineage of esophageal skeletal muscle differentiation was established by an immunotyping approach, which revealed that skeletal myocytes arise from a distinct lineage rather than through transdifferentiation of smooth muscle cells during rat esophageal myogenesis.     

中枢神经系统原发的恶性淋巴瘤

本文总结了８例中枢神经系统原发的恶性淋巴瘤，分析其临床病理特点。８例肿瘤均位于大脑且为弧立的或弥漫多中心的病灶。组织学类型均为弥漫型，以小细胞性及低度恶性为多。ＬＣＡ，ＣＤ（２０）及ＣＤ（４３）免疫组织化学染色证实中枢神经系统恶性淋巴瘤多为Ｂ细胞性淋巴瘤。     

Mediastinal Clear Cell Lymphoma—A Distinct Entity of B-Cell Derived Lymphoma as Shown by Immunotyping and Analysis of Gene Rearrangements

Mediastinal clear cell lymphoma (MCCL) is regarded as a distinct subtype of Non-Hodgkin's-lymphoma. We have analyzed gene rearrangements of eight cases of newly diagnosed MCCL in order to verify their alignment to the B-cell lineage as suggested by immunotyping. The lymphoma cells shared a common immunophenotype which consisted of CD10-, CD19+, CD20+, and CD21-. Rearrangements of the heavy chain of the immunoglobulin gene were found in all eight cases studied. In contrast, the beta chain of the T-cell antigen receptor gene was not rearranged. No rearrangements of the kappa light chain gene were detected. There was no evidence for a t(14;18) or a t(8;14). In conclusion, MCCL seems to be a B-cell derived tumor, which is distinct from other known lymphomas both by its immunophenotype and genotype.