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目的:探测血清透明质酸(HA)与层粘连蛋白(LN)的联合检测在急、慢性肝病中的作用。方法:采用放射免疫法测定正常对照组18例及急、慢性肝病患者76例HA、LN的含量,结果76例急、慢性肝病患者血清HA、LN与正常对照比较,差异有显著性(P〈0.01)。结论:以HA、LN为实验参考指标,对尽早发现慢性肝病向肝硬化转化起到早预防、早诊治的作用。 相似文献
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Structure, distribution and composition of the extracellular matrix of human oocytes and cumulus masses. 总被引:4,自引:0,他引:4
The structure, distribution and composition of the extracellular matrix present around the human oocyte and in the cumulus was examined following fixation in the presence of ruthenium red. An extracellular matrix comprising granules and filaments is present in the cumulus layer, in the corona radiata, in the outer pores of the zona pellucida and in the perivitelline space surrounding unfertilized oocytes. In replicate samples, the extracellular matrix comprised filaments which were mostly very long, occasionally cross-connected by shorter filaments, and usually decorated with numerous small granules. Enzymatic digestion with affinity-purified trypsin or Streptomyces hyaluronidase removes the granules and filaments, respectively, at all levels of the oocyte-cumulus complex. These results are interpreted to mean that protein and hyaluronic acid are present in all extracellular compartments of the human oocyte-cumulus complex. The significance of this distribution of hyaluronic acid with respect to the role of sperm hyaluronidase in fertilization is discussed. 相似文献
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