In vivo electrochemical methods were employed to study the potassium (K+-evoked release of monoamines from the cerebellum of the chloral hydrate anesthetized rat. K+-evoked releases were elicited using micropipette-Nafion-coated graphite epoxy electrode arrays in the granule/Purkenje cell layer, molecular layer, and white matter. These recorded releases were generally found to be reversible, moderately dose-dependent, and reproducible. However, the temporal dynamics of the releases were different for the cell layer versus molecular layer records. Releases were infrequently observed in cerebellar white matter, an area which is relatively devoid of monoamine containing terminals. The signals recorded from the cell and molecular layers were significantly attenuated by pretreatment with nomifensine, a potent catecholamine reuptake blocker, significantly prolonged the K+-evoked signals observed in both the granule/Purkenje cell and molecular layers. These data, taken together with earlier reports on the electrophysiological responses to activation of cerebellar noradrenergic inputs, support the conjecture that in vivo electrochemical recording methods have the sensitivity and spatial resolution for studies of functional monoamine release from brain regions that have a diffuse or laminated monoamine innervation. 相似文献
Summary: A new thin‐film characterization setup was created based on the combination of a surface plasmon spectrometer with an electrochemical cell operated under high pressure of up to 200 MPa and at temperatures up to 120 °C. The examples given to document its performance include photoisomerization studies with poly(methyl methacrylate) (PMMA) films partly derivatized with disperse red (DR1), as well as, a preliminary account of the electropolymerization of EDOT under pressure and the assessment of the redox properties of the resulting thin PEDOT films.
Sketch of the high‐pressure electrochemistry surface plasmon cell. 相似文献
The polycycloaddition of azomethine ylides to dipolarophiles is described for the first time. Use of (cis,cis)‐bis‐aziridines as a source of azomethine ylides allows selective polyaddition to be realized, which leads to oligomers exclusively with trans‐substituted pyrrolidine rings and modest molecular weights (ca. 5–10 kDa). The fluorescent, electrochemical, electrochromic, and non‐linear optical properties of the main‐chain free‐base porphyrin oligomer, synthesized by the developed procedure, are studied. An enhancement of the optical power limiting effect in a porphyrin oligomer solution is registered against that of the monomer.