Cancer-salient messaging for Human Papillomavirus vaccine uptake: A randomized controlled trial

Background and objectivesVaccination with Human Papillomavirus (HPV) vaccine is recommended for 11–12 years-old, but uptake is suboptimal. Current messaging focuses on HPV infection transmission and prevention. Parents and providers are often uncomfortable discussing sexual practices of adolescents, contributing to the delay/refusal of vaccine. We created a cervical cancer-salient message encouraging HPV vaccination, emphasizing disease salience and disease threat, while promoting self-efficacy. We hypothesized this message would have greater effects on vaccine confidence and intent to vaccinate compared to Centers for Disease Control and Prevention (CDC) and non-vaccine control messages.MethodsA 3-arm randomized trial was conducted. Parents of girls aged 9–17 were eligible for the study. We measured participants’ vaccine confidence and intent to vaccinate at baseline and post intervention message. Recruitment and surveys were administered online through Amazon Mechanical Turk.Results762 participants completed both surveys. We saw modest increases in vaccine confidence when comparing cervical cancer arm and control arm, and CDC arm and control arm; estimates were not statistically significant. The odds of reporting intent to vaccinate among the cervical cancer message arm were 1.13 times the odds of reporting intent to vaccinate in the control arm (95% CI: 0.30. 4.29). Intent to vaccinate was also not statistically significantly different between CDC message arm and control arm (OR = 1.25, 95%CI: 0.66, 2.37).ConclusionNeither message had effect on intent to vaccinate, highlighting need for research to identify successful messaging strategies for HPV. Exploratory analyses suggest among parents with ‘Low’ vaccine confidence at baseline, the cervical cancer framed message may be more effective in changing intention than the CDC message or non-vaccine control. Future work should target groups with ‘Low’ or ‘Medium’ vaccine confidence at baseline - they may be more amenable to change, and more receptive to disease-salient messaging.Clinical Trial Registration: Clinicaltrials.gov, Reference #: NCT03002324.     

可见光及紫外光分光光度法测定丁香总黄酮含量的研究

目的研究可见光及紫外分光光度法测定丁香总黄酮含量的方法。方法可见光分光光度法是以芦丁为参照品,硝酸铝作显色剂,在510 nm波长测定总黄酮含量。紫外分光光度法是以芦丁为参照品,在359 nm波长测定总黄酮含量。结果可见光法平均回收率是99.46%,紫外光法是99.13%。RSD分别是1.15%和0.90%。结论紫外分光光度法是一种较理想的测定方法。     

不同分子量的聚乙烯醇载药膜片体外溶出度测定

 采用可见紫外分光光度法对不同聚合度及质量比的聚乙烯醇（ＰＶＡ）制成的含有硫酸阿托品及甲硝唑载药膜，在不同溶出介质中的溶出度进行测定、比较，为制备ＰＶＡ缓释载药膜片，选择适宜的质量比及相应的聚合度，提供参考依据。     

2008～2012年临床生化室间质量评价结果分析

目的通过对临床生化检验室在2008～2012年期间参加安徽省临床生化检验室间质评结果的回顾分析和总结,不断提高检验质量,从而更好地为临床服务。方法将5 a室间质评结果统计,按卫生部临检中心统一评价标准,同时采用PT(能力验证)和VIS(变异指数得分)两种评分方法进行评分。结果 5 a平均PT合格率达95.8%,VIS得分优秀占83.9%,合格占10.5%,不合格占5.6%。结论通过开展室间质评工作,提高了实验室人员的质控意识,改进了分析能力和实验方法,从而提高了检验质量。     

Vasoactive Inotropic Score as a Predictor of Mortality in Adult Patients With Cardiogenic Shock: Medical Therapy Versus ECMO

Introduction and objectives

This study investigated whether the vasoactive inotropic score (VIS) is independently predictive of mortality in cardiogenic shock (CS).

Estimation of the relative stratum corneum amount removed by tape stripping

BACKGROUND/PURPOSE: The tape stripping procedure is a suitable minimal invasive tool to study, e.g. the penetration and dermatopharmacokinetics of topically applied substances. In the present study, this procedure was used to remove the stratum corneum (SC) completely and to study the penetration of the UVA filter substance butyl methoxydibenzoylmethane after application in two different vehicles. METHODS: The amount of corneocytes removed by each tape strip from the flexor forearm of human volunteers was determined via their pseudo-absorption. In a second part, the penetration profiles of a UVA filter substance applied in two different vehicles were determined following the developed standard protocol using the tape stripping procedure in combination with UV/VIS spectroscopy. RESULTS: The amount of corneocytes removed by each tape strip was related to the number of tape strips used for removal. Mean values with a deviation of less than 20% concerning the relative amount of SC removed by a constant number of tape strips were obtained. For instance, a relative amount of 66 +/- 12% was removed with the first 20 tape strips, while nearly the complete SC (95 +/- 3%) was removed using 50 tape strips. In addition, these results were used to estimate the relative SC amounts removed, studying the penetration of the UVA filter substance after application in two different vehicles. No significant differences between the distributions of the UV filter substance applied in both emulsions were obtained (P > 0.05). CONCLUSIONS: The reported procedure for the estimation of the removed SC amount provides the possibility to avoid the complete removal of the SC and to compare the penetration characteristics obtained for different volunteers and different products in relation to the relative horny layer profile.     

Development and applications of photo-triggered theranostic agents

Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.     

聚酰胺树脂分离纯化芦荟蒽醌衍生物

目的 研究纯化芦荟蒽醌衍生物的工艺条件.方法 对照乙醇梯度洗脱所得各组分的HPLC谱图,考察聚酰胺树脂富集、纯化芦荟蒽醌的吸附性能和洗脱参数.结果 通过聚酰胺树脂富集与纯化,并采用40%,60%,70%,80%乙醇梯度洗脱,可以使芦荟蒽醌衍生物得到较好的分离.结论 该法的精确度、稳定性、重复性和回收率都较好,且操作简便易行.