全文获取类型
收费全文 | 66篇 |
免费 | 1篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 2篇 |
妇产科学 | 1篇 |
基础医学 | 11篇 |
口腔科学 | 2篇 |
临床医学 | 8篇 |
内科学 | 6篇 |
神经病学 | 1篇 |
外科学 | 11篇 |
综合类 | 5篇 |
预防医学 | 3篇 |
药学 | 2篇 |
肿瘤学 | 16篇 |
出版年
2022年 | 2篇 |
2020年 | 2篇 |
2019年 | 7篇 |
2018年 | 8篇 |
2017年 | 2篇 |
2015年 | 1篇 |
2014年 | 4篇 |
2013年 | 4篇 |
2012年 | 4篇 |
2011年 | 3篇 |
2010年 | 4篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 1篇 |
2006年 | 1篇 |
2005年 | 1篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1991年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有68条查询结果,搜索用时 17 毫秒
1.
EB病毒VCA-IgA抗体水平与鼻咽癌病人远期疗效关系 总被引:6,自引:1,他引:6
目的:分析EB病毒血清学VCA-IgA抗体与鼻咽癌病人远期疗效关系,为临床治疗提供参考依据。方法:1985年本院病理确诊的522例鼻咽癌病人,按治疗前、后VCA-IgA抗体滴度分为三组:低滴度组(1:5~1:20)、中滴度组(1:40~1:80)和高滴度组(1:160以上),随访10年,比较生存率的异同。结果:治疗前鼻咽癌病人高滴度组10年生存率低于低滴度组(P<0.05),但和中滴度组差别无显著性(P>0.05)。治疗后三组病人10年生存率比较,高、中滴度二组差别无显著性(P>0.05),但低于低滴度组。结论:EB病毒VCA-IgA抗体水平可以作为估计预后的重要参考依据,与远期疗效有密切关系。 相似文献
2.
目的 通过比较EB病毒抗体检测试剂盒血清学诊断鼻咽癌的准确性和检测结果的一致性,为试剂盒在临床上的使用选择和性能改进提供依据.方法 使用五厂家的EB病毒衣壳抗原IgA和IgG抗体检测试剂盒(VCA IgA和VCA IgG试剂盒)、核抗原I IgA和IgG抗体检测试剂盒(EBNA1 IgA和EBNA1IgG试剂盒)、早期抗原IgA和IgG抗体检测试剂盒(EA IgA和EA IgG试剂盒)以及Zta IgA抗体检测试剂盒,分别检测33例鼻咽癌患者(NPC)、30例健康体检者(HD)和41例非鼻咽癌的其他肿瘤患者(NNPC)血清或血浆样本.结果 A厂家的VCA IgA试剂盒灵敏度高于其他厂家同品种试剂盒,但对于NNPC特异度最低(36.6%);而D厂家VCA IgA试剂盒的特异度最高(97.6%),且对HD的特异度均大于90%.B和D厂家的EBNA1 IgA试剂盒间阳性、阴性符合率分别为92.1%和100.0%.A和E厂家的EA IgA试剂盒的灵敏度均较低而特异度高,试剂盒间阳性符合率低(39.4%),阴性符合率高(98.6%);而VCA IgG试剂盒的灵敏度高但特异度低.A和C厂家的EBNA1IgG试剂盒的灵敏度高(100.0%,97.0%)但特异度低(3.3%,13.3%).C厂家EA IgG试剂盒检测所有样本结果均为阴性.结论 五个不同厂家VCA LgA、EA IgA试剂盒诊断鼻咽癌的准确性和检测结果的一致性存在差异,特别是A厂家和其他国内厂家同品种试剂间差异明显,需根据临床目的进行选择.三家国产VCA IgA试剂盒的灵敏度需进一步提高.相反,EBNA IgA试剂盒诊断鼻咽癌的准确性和结果一致性较好.单独使用VCA IgG和EBNA1 IgG试剂盒血清学诊断鼻咽癌的特异度差,其判读界值可能需根据检测且的进行调整. 相似文献
3.
孙延辉 《中国组织工程研究与临床康复》2010,14(17)
背景:为使深度不同但性质相同的界面反射的回波信号能显示出相同的幅值,必须根据深度(即时间)逐步增大放大器的增益,而使超声波在传播衰减过程中所引起远距离反射波弱的情况得到相应补偿.目的:为了补偿医学超声系统中回波信号的传输衰减问题,提出了一种时间增益补偿电路设计方案,阐述电路设计依据和原理.方法:针对医用超声系统的特点选择高信噪比、高带宽的可变增益放大器件VCA610,实现超声的增益补偿电路.结果与结论:该设计方案有效地解决了医用超声软组织测量过程中由声程导致的回波信号的非线性补偿问题.与传统的分立元件电路相比,该方案具有电路简单,TGC控制信号稳定可靠以及调节灵活等优点,能准确地补偿超声波在人体内的衰减,从而为医学测量系统的设计提供了一个新的可靠方法. 相似文献
4.
This report describes a vasculitis and subsequently developing angiodestructive lymphoma in an 11-year-old Japanese-Filipino girl exhibiting mosquito allergy with the background of chronic active Epstein-Barr virus (EBV) infection. She developed necrotic skin ulcer at the site of mosquito bite, and histopathological examination revealed EBV-positive mononuclear cell infiltration throughout the wall of small-sized muscular artery. These EBV-positive lymphoid cells were oligoclonal in Southern blot analysis for EBV terminal repeats. Effectiveness of steroid therapy also supports the nonneoplastic nature. Approximately 1 year later, she developed progressive large skin ulcer without mosquito bites. Microscopically, the angiocentric or angiodestructive pattern of EBV-positive atypical cells supported the diagnosis of extranodal natural killer/T-cell lymphoma. Southern blot analysis revealed the monoclonal neoplastic nature of EBV-positive cells. In contrast to the primary mosquito bite lesion, natural killer/T-cell lymphoma cells exhibited the higher expression of EBV latent membrane protein 1 mRNA and the apparent protein expression detected by immunohistochemistry. 相似文献
5.
鼻咽癌患者发病前后EB病毒VCA/IgA和EA/IgA滴度动态分析 总被引:8,自引:0,他引:8
目的 观察鼻咽癌患者发病前后EB病毒VCA/IgA、EA/IgA滴度的变化规律,及其在鼻咽癌筛查中的作用。方法 收集中山市首次鼻咽癌筛查后12年VCA/IgA阳性人群中54例新发鼻咽癌患者发病前后的血清学资料,用免疫酶法检测EB病毒抗体VCA/IgA和EA/IgA。结果 确诊前1~7年VCA/IgA、EA/IgA总体呈上升趋势。发病前7~4年,VCA/IgA平均滴度在1:21.04上下波动,确诊前第3年起VCA/IgA急剧上升,确诊时几何平均滴度接近1:80,EA/IgA高较为缓慢,确诊时几何平均滴度为1:6.49。放疗后两种滴度均呈快速下降趋势,第4年起接近阳性人群的平均滴度。结论 多数鼻咽癌患者在确诊前3年,VCA/IgA滴度持续增高,但EA/IgA滴度增高缓慢;VCA/IgA可以检出早期鼻咽癌,但EA/IgA作用不大;鼻咽癌发展临床前期平均时间为3年。 相似文献
6.
Holger J. Klein Fabienne Lehner Riccardo Schweizer Barbara Rüsi-Elsener Jakob Nilsson Jan A. Plock 《Burns : journal of the International Society for Burn Injuries》2018,44(5):1330-1335
Background
Since the inception of clinical VCA almost two decades ago, burn victims have been identified as immunologically complex patients owing to preformed HLA antibodies. However, it remains unclear whether the detected HLA antibodies are the result of former alloantigenic events or if their de novo formation occurs during primary burn care.Methods
Patients with burns >20% of total body surface area (TBSA) admitted to Zurich Burn Centre between May 2015 and September 2016 were screened for HLA antibodies at admission and 6 months post trauma. The influence of glycerol-preserved donor skin and red blood cell transfusion on HLA antibody formation was investigated.Results
Thirty-seven patients (7 females and 30 males) with an average age of 45.2 ± 19.1 years and average affected TBSA of 38.5% ± 18.9% were screened for HLA antibodies. Five patients (13.5%) showed preformed HLA antibodies at admission. Only 3 patients demonstrated verified de novo sensitization during acute burn care. Six patients treated with donor skin and blood transfusions showed no occurrence of HLA antibodies after 6 months. One patient died after 2 weeks due to infectious and thromboembolic complications – however, HLA antibodies were already detected.Conclusion
Formation of HLA antibodies during acute burn care might be lower than that previously expected by using glycerol-preserved donor skin and restrictive administration of blood products. Modern concepts of burn care may contribute to a revised outlook on burn patients as potential candidates for VCA. 相似文献7.
8.
Eva Sickinger Mario Berth Angela Vockel Hans-Bertram Braun Michael Oer Carsten Buenning 《Diagnostic microbiology and infectious disease》2014
In the current evaluation, Epstein-Barr virus (EBV) serology was performed on 1113 routine serum samples. Although the initial request for all samples from the general practitioner was EBV IgM testing, 80.9% were classified as past infections. The ARCHITECT® viral capsid antigen (VCA) IgM, VCA IgG, and EBV nuclear antigen (EBNA) 1 IgG assays showed good results for sensitivity and specificity, being 100.0%, 98.3%, and 100.0% and 99.9%, 95.4%, and 99.6%, respectively. Using an algorithm based on initial EBNA-1 IgG testing, followed by VCA IgG and IgM for samples that were not EBNA-1 IgG reactive, the number of tests per sample could be reduced to nearly 50% compared to parallel testing. The high sensitivity and specificity of the ARCHITECT® EBNA-1 IgG assay in combination with a low number of grayzone results are a precondition for the chosen test algorithm. Thus, the newly developed ARCHITECT® EBV panel is suitable for accurate and cost-efficient EBV serology in a routine clinical laboratory. 相似文献
9.
Lymphocyte abnormalities in preleukemia--I. Decreased NK activity, anomalous immunoregulatory cell subsets and deficient EBV receptors 总被引:1,自引:0,他引:1
R W Anderson D J Volsky B Greenberg S J Knox T Bechtold C Kuszynski S Harada D T Purtilo 《Leukemia research》1983,7(3):389-395
The development of acute non-lymphocytic leukemia is preceded by a set of symptoms described as the preleukemia syndrome. Six patients who fulfilled the criteria for this preleukemia syndrome have been evaluated for abnormalities in the lymphocyte population. The NK cell activity was reduced, the immunoregulatory cell populations were numerically abnormal, and the B cell subpopulation was deficient in EBV receptors. Thus, in addition to the abnormalities in the myeloid populations, there are serious defects in the lymphoid systems of preleukemic patients. 相似文献
10.
Holden JK Nguyen RH Francisco EM Zhang Z Dennis RG Tommerdahl M 《Journal of neuroscience methods》2012,204(2):215-220
Current methods for applying multi-site vibratory stimuli to the skin typically involve the use of multiple, individual vibrotactile stimulators. Limitations of such an arrangement include difficulty with both positioning the stimuli as well as ensuring that stimuli are delivered in a synchronized and deliberate manner. Previously, we reported a two-site tactile stimulator that was developed in order to solve these problems (Tannan et al., 2007a). Due to both the success of that novel stimulator and the limitations that were inherent in that device, we designed and fabricated a four-site stimulator that provides a number of advantages over the previous version. First, the device can stimulate four independent skin sites and is primarily designed for stimulating the digit tips. Second, the positioning of the probe tips has been re-designed to provide better ergonomic hand placement. Third, the device is much more portable than the previously reported stimulator. Fourth, the stimulator head has a much smaller footprint on the table or surface where it resides. To demonstrate the capacity of the device for delivering tactile stimulation at four independent sites, a finger agnosia protocol, in the presence and absence of conditioning stimuli, was conducted on seventeen healthy control subjects. The study demonstrated that with increasing amplitudes of vibrotactile conditioning stimuli concurrent with the agnosia test, inaccuracies of digit identification increased, particularly at digits D3 and D4. The results are consistent with prior studies that implicated synchronization of adjacent and near-adjacent cortical ensembles with conditioning stimuli in impacting TOJ performance (
[Tommerdahl et al., 2007a] and [Tommerdahl et al., 2007b]). 相似文献