胸、腹水中测定G-CSF的意义

目的观察胸、腹水中G—CH的水平及意义。方法应用中国医学科学院生产的粒细胞集落刺激因子（G—CSF）酶联免疫检测试剂盒对140例胸、腹水标本中G－CSF水平进行检测。结果漏出液组60例，G－CSF阳性检出度为3．3％，渗出液组80例，G－CH阳性检出率为77．5％，经x2检验具有显著性差异，且渗出液阳性标本WBC＞2．0X109／L，以中性粒细胞为主，提示感染性胸、腹水，渗出液18例阴性标本中，WBC计数在0．5—1.2X109／L之间，分类以淋巴细胞为主，提示非感染性渗出液。结论检测胸、腹水中的G－CSF可以鉴别渗出液和漏出液，且可作为诊断感染性渗出浓的重要依据。     

创面收集液对表皮干细胞表型的影响

目的 探讨全层创面收集液对表皮干细胞类型的影响。方法 以聚氨酯海绵收集成年Wistar大鼠背部全层创面渗出液；以快速黏附分离法体外分离、培养新生Wistar大鼠的表皮干细胞，待表皮干细胞呈克隆生长时加入创面收集液干预，分别于干预后的0，6，12，18，24，30，36，42，48，54，60，72h采用流式细胞仪及β1整合素、角蛋白19，14，10免疫组化染色进行细胞增殖分化的鉴定。结果表皮干细胞在创面收集液干预后仍可持续保持片状聚集生长的态势，但出现数量较多的角蛋白14阳性染色细胞群落，且随时间延长该阳性细胞群落呈上升趋势；另出现了散在的角蛋白10染色阳性细胞。结论创面收集液可以快速诱导体外培养表皮干细胞进入分化状态，且多表现为短暂扩充细胞表型，在该过程中表皮干细胞数量仍可维持在一定的水平。     

Pathogenesis of Rushton bodies: A novel hypothesis

Rushton bodies (RBs) are one of the characteristic features seen in the epithelial lining of odontogenic cysts mainly radicular, dentigerous and odontogenic keratocyst. It has two different histo-morphological appearances; granular and homogeneous. Although widely investigated, the exact pathogenesis and histogenesis of RBs is still an enigma. Many hypotheses were made in the literature but none has explained conceivably the two histo-morphological appearances of RBs and their association with inflammation. In the present paper the various pathogenesis for the formation of RBs proposed till date are discussed along with proposal for a novel hypothesis. The given hypothesis is mainly related to inflammation and its effect on pore size of basement membrane of odontogenic cystic epithelium. It explains RBs association with inflammation as well as existence of two histo-morphological appearances. The proposed hypothesis also justifies the RB’s presence inside the lining epithelium of odontogenic cyst despite its hematogenous origin. Future studies are advocated for isolating RBs using laser capture microdissection and subsequent biochemical, histochemical and electron microscopic analysis to substantiate the proposed hypothesis.     

腹水胆固醇及其它生化指标检测对腹水诊断的意义

腹水胆固醇测定对腹水性质的判定有高度的敏感性和特异性,渗出液和漏出液之间没有重叠,界值在30mg/dl,凡腹水胆固醇超过30mg/dl者,均系渗出性腹水。但不能鉴别炎性及癌性腹水。腹水蛋白亦有诊断意义,其它如乳酸脱氢酶、乳酸、葡萄糖仅有参考意义。     

多指标联合检测鉴别渗出与漏出性胸腔积液的研究

目的 检测胸腔积液患者胸腔积液胆固醇( PE CHOL)、胸腔积液胆固醇与血清胆固醇比值(P/S CHOL)、胸腔积液C-反应蛋白(PE CRP)和血清-胸腔积液白蛋白差(SEAG),并与Light标准进行比较,探讨多指标联合检测在鉴别渗、漏出性胸腔积液中的价值.方法 将137例不同病因的胸腔积液患者分成漏出液组和渗出...     

Effect of iatrogenic haemorrhage on biochemical parameters in pleural effusions

AIM: Pleural fluid (PF) samples may become haemorrhagic due to trauma during diagnostic procedures. We aimed to evaluate the effect of increasing blood concentrations in pleural fluids on biochemical parameters which are used to discriminate transudates and exudates. METHODS: Sixty-seven pleural fluid samples were separated into five test tubes. Patient's own blood was added in the test tubes in different concentrations as follows: No blood in the first tube, 2% in the 2nd, 5% in the 3rd, 10% in the 4th, 20% in the 5th tube. RESULTS: After addition of blood, statistically significant changes in all biochemical parameters in transudate groups were detected. The characteristics changed from transudate to exudate 12.9%, 14%, 11.4%, 27% and 14.3% according to Light's criteria, serum-PF albumin gradient, cholesterol level, PF/serum cholesterol and PF/serum bilirubin, respectively. Results indicating an exudate according to Light's criteria were evident only in the 5th tube group, however, when other biochemical parameters were used, classification of samples could be altered with lower concentrations of blood. CONCLUSION: Contamination of blood, especially in borderline transudative pleural effusions, may result in misclassification as an exudate. Light's criteria appear to be the least effected and therefore the most reliable parameters in bloody effusions.     

78例老年胸腔积液临床特点分析

目的分析老年胸腔积液患者的临床特点,总结诊断胸腔积液类型的方法。方法对我院2004～2007年收治的78例已明确诊断的老年胸腔积液患者的临床资料进行分析。结果经病理及临床治疗经过证实,老年胸腔积液以恶性胸腔积液(占47.4%)及结核性胸膜炎(占38.5%)居多,其他病因较为少见。结论老年胸腔积液病因复杂,临床表现不典型,鉴别较为困难,需结合多方检查资料及治疗疗效综合分析,选择可靠方法明确诊断。     

中性溯胞弹性蛋白酶(NE)在鉴别渗出液和漏出液中的意义

目的 探讨中性粗细胞弹性蛋白酶（NE）在鉴别渗出液和漏出液中的作用。方法 运用ELISA法检测100份浆膜腔积液中的NE。其中50份为渗出液，50份为漏出液（经传统显微镜法，化学方法及结合临床判断）。结果 92％渗出液NE浓度值均大于290ng／ml；96％漏出液NE浓度值均小于290ng／ml。渗出液NE显著高于漏出液（P〈0．05）。结论 NE在鉴别渗出液和漏出液中有重要意义。