M-sol血小板保存液对血小板体外保存期间质量的影响

目的探讨M-sol血小板添加液对保存期血小板质量的影响。方法采集健康献血者标本5份,制备浓缩血小板,应用M-sol血小板添加液保存浓缩血小板,以富含血小板的血浆(血浆组)作对照,分别在保存1、5、7、10d检测血小板计数、平均血小板体积(MPV)、胶原诱导的聚集反应及葡萄糖消耗和乳酸生成。结果 M-sol保存组血小板溶液的pH、pCO2比血浆组稳定,血小板聚集率下降幅度比血浆组小,葡萄糖消耗和乳酸生成幅度低于血浆组。结论 M-sol添加液在血小板的体外保存期维持血小板质量的作用优于血浆。     

Comparison between in vitro properties of washed platelet concentrates suspended in M-sol and those in BRS-A,both of which were prepared with an automated cell processor

Background

Washed platelet concentrate (WPC) is prepared manually in general, but automated preparation is desirable to minimize variation in the WPC quality and enhance WPC production. Recently, the software was improved for an automated cell processor (ACP) to control all processes of WPC preparation. M-sol and BRS-A, which are mixtures of medical solutions, are widely used for WPC preparation with a manual method in Japan. In this study, we prepared WPC suspended in M-sol (WPC-M) or BRS-A (WPC-B) with the ACP, and compared their in vitro properties during 7-day storage.

3种不同洗液洗涤血小板的比较及其保存的体外实验研究

[摘要]目的：观察3种不同的洗液（生理盐水,ACD-A保养液和PASllIM）对机采血小板质量和功能的影响;同时观察洗涤血小板保存于PASⅢM和M-sol后3d其功能及活化程度的变化。方法：机采血小板分别用生理盐水,ACD-A保养液和PASⅢM洗涤血小板,洗涤后分别保存于PASⅢM和M-sol中观察0、24、48、72h血小板的功能变化及激活程度的差异。用血小板计数仪测血小板数量,PMV、PDW;用比浊法测血小板聚集性;用流式细胞仪测血小板表面CD62P的表达情况。结果：①PASⅢM组CD62P的激活率较低,与其他2组分别相比差异有统计学意义（P〈O．05）。②洗涤后血小板分别保存于PASⅢM和M-sol保养液中保存24h和48h后,所有的检测数据与对照组相（0h）比较差异无统计学意义,2组之间比较差异无统计学意义,且在正常的范围之内;③保存72h后血小板聚集性与对照组相比差异有统计学意义（P〈0．01）,2组之间比较差异无统计学意义;而血小板CD62P的激活率在2种保存液之间差异有统计学意义。结论：3组洗液比较,PASⅢM对血小板功能及激活程度影响最小,是较理想的洗液;在PASⅢM和M-sol保存液中保存48h,血小板的质量指标仍在正常范围之内;洗涤血小板72h,M-sol保存液中血小板的功能指标优于PASⅢM中的。