PACS与HIS／RIS集成网关的研究与设计

本论文针对目前医院信息化建设中急需解决的PACS与HIS/RIS网关进行了研究，研究PACS如何同HIS/RIS系统进行信息交互方式，升给出了PACS与HIS／RIS网关的设计和实现方案。     

TAGLN真核表达载体pcDNA6.2/EmGFP-Bsd/V5-TAG-LN-mut及稳定转染细胞株的构建

目的:构建携tagln基因的真核表达载体,建立稳定转染该质粒的人结肠癌细胞株RKO并鉴定.方法:通过Gateway克隆技术,使用pOTB7-TAGLN-mut与pDONR221进行BP重组反应产生入门克隆,再与pcDNA6.2/EmGFP-BsdV5-DEST空载体进行LR重组反应,生成目的质粒pcDNA6.2/EmGFP-Bsd/V5-TAGLN-mut通过测序验证目的质粒的插入序列.将携带tagln的真核表达载体和对照质粒稳定转染至人结肠癌细胞株RKO.通过实时荧光定量PCR和免疫印迹检测tagln的mRNA和蛋白表达水平.通过细胞侵袭实验了解transgelin在结肠癌细胞RKO中的作用.结果:携带tagln的真核表达载体测序分析显示插入序列及位点正确;实时荧光定量PCR及免疫印迹结果显示,稳定转染重组目的质粒的细胞株(RKO-TAGLN细胞)中tagln的表达水平与转染对照质粒的细胞株(RKO-CTRL细胞)及野生型RKO细胞相比明显上调,差异具有统计学意义(mRNA相对表达水平分别为45.58±12.79、1.32±0.43和1,P<0.01;蛋白质灰度定量值为1.69±0.04、0.29±0.05和0.29±0.04,P<0.01).细胞侵袭实验提示,RKO-TAGLN细胞较RKO-CTRL细胞的侵袭能力提高(161.76%±61.18%,P<0.01).结论:成功构建携tagln基因的真核表达载体并建立过表达transgelin的稳定细胞株和对照细胞株,为研究transgelin在结肠癌中的作用奠定基础.     

电子医药期刊全文检索策略的探讨

医药期刊全文是医学和药学科研与实践过程中重要的参考文献,本文对目前国内外主要电子医药期刊全文的数据源以及检索途径和方法进行了分析和探讨,以期为医药从业者获取相关文献信息提供参考。     

Identification of Youth Psychosocial Problems During Pediatric Primary Care Visits

This investigation applied the Gateway Provider Model (GPM) of child mental health services to investigate whether characteristics of the child, family, visit, and provider were related to the identification of youth psychosocial problems during primary care visits. Data were gathered during 774 visits to 54 primary care providers (PCPs) at 13 clinics. Similar to previous investigations in primary care settings, 42% of youth demonstrated at least a sub-threshold clinical mental health problem. Most PCPs reported high job satisfaction and control, but reported varying access to mental health specialists. PCPs generally had positive attitudes and beliefs about treating psychosocial problems but many reported that doing so was burdensome. Identification was more likely when the visit was for a mental health problem, when issues related to psychosocial problems were discussed during the visit, when the youth demonstrated mental health symptoms, impairment, or burden to the family, and when the youth was older, uninsured, or received Medicaid. Identification was less likely when the PCP reported greater burden associated with treating psychosocial problems and when the PCP reported greater accessibility to mental health specialists. These results suggest that identification is associated with the interaction of multilevel factors and that the GPM is a useful model to investigate points of intervention for improving the identification of children’s mental health problems in primary care settings. Presented in part at the University of South Florida 18th Annual Research Conference: A System of Care for Children’s Mental Health, Tampa, FL, February 2006.     

The NIF LinkOut Broker: A Web Resource to Facilitate Federated Data Integration using NCBI Identifiers

This paper describes the NIF LinkOut Broker (NLB) that has been built as part of the Neuroscience Information Framework (NIF) project. The NLB is designed to coordinate the assembly of links to neuroscience information items (e.g., experimental data, knowledge bases, and software tools) that are (1) accessible via the Web, and (2) related to entries in the National Center for Biotechnology Information’s (NCBI’s) Entrez system. The NLB collects these links from each resource and passes them to the NCBI which incorporates them into its Entrez LinkOut service. In this way, an Entrez user looking at a specific Entrez entry can LinkOut directly to related neuroscience information. The information stored in the NLB can also be utilized in other ways. A second approach, which is operational on a pilot basis, is for the NLB Web server to create dynamically its own Web page of LinkOut links for each NCBI identifier in the NLB database. This approach can allow other resources (in addition to the NCBI Entrez) to LinkOut to related neuroscience information. The paper describes the current NLB system and discusses certain design issues that arose during its implementation.     

应用Gateway技术构建pLenti6-Akt shRNA真核表达载体

目的 应用Gateway技术构建靶向人Akt基因的shRNA真核表达载体,转染肝癌细胞株HepG2,验证该载体能否下调Akt基因的mRNA水平.方法 以Akt基因为靶点设计两条具有短发卡结构的shRNA序列,经退火成互补双链后克隆入pENTR/U6入门表达载体(pENTR/U6-Akt1,pENTR/U6-Akt2);转化E.coli TOP10菌株,挑取阳性菌落进行菌落PCR和测序鉴定;利用Gateway技术进行LR位点特异性重组,将shRNA导入目的 表达载体pLenti6/Block-it DEST Vector,再行测序鉴定(pLenti6-Akt1,pLenti6-Akt2);将重组质粒转染肝癌细胞株HepG2,提取RNA,RT-PCR检测重组质粒对mRNA水平的抑制效果.结果 经菌落PCR和DNA测序鉴定:pENTR/U6-Akts入门载体和pLenti6-Akts目的 表达载体中插入片段的序列正确,RT-PCR结果表明将shRNA分别转染HepG2细胞后,Akt的mRNA表达受到明显抑制,其中1shRNA比2#shRNA抑制效果明显.结论 利用Gateway技术成功构建了靶向人Akt基因的shRNA表达载体,为以Akt基因为靶点的肝癌基因治疗方案奠定了实验基础.     

Consumo actual de cigarrillos electrónicos entre estudiantes de secundaria que nunca han fumado

ObjectiveTo test whether electronic cigarettes use among secondary school students in Spain is associated with initiating nicotine use.MethodSecondary analysis of the ESTUDES 2019 survey, a cross-sectional study conducted on a representative sample of students aged 14–18 years and selected students who had never smoked (n = 16,705). We computed the prevalence and estimated the factors associated with current electronic cigarettes use (at least once in the month before the interview), with and without nicotine.ResultsThe prevalence of current electronic cigarettes use among students who have never smoked is 2.5% (95% confidence interval [95%CI]: 2.2–2.9). 0.1% (95%CI: 0.08–0.2) declared using liquids with nicotine at least sometimes and 2.4% (95%CI: 2.1–2.8) using liquids always without nicotine. Being male and under 17 years of age, reporting binge drinking behavior, and having regular friends who use cannabis, increases the probability of current use of electronic cigarettes. The latter two variables seem to act as contextual variables associated with electronic cigarettes use.ConclusionsThe percentage of Spanish never smoker students who currently use electronic cigarettes is lower than in neighboring countries, except for the United Kingdom. Only one in every 1000 Spanish students between 14 and 18 years old who have never smoked initiates the current use of nicotine with an electronic cigarette. However, we must monitor the evolution of this indicator.     

Relationship between Adolescent Marijuana Use and Young Adult Illicit Drug Use

We examined three components of the “gateway theory” in relation to marijuana use: (1) whether adolescent marijuana use predicts young adult drug use, (2) whether this association persists when controlling for similar family background, (3) whether common genetic or environmental factors contribute to the association. The three components were tested in adolescents from the National Longitudinal Study of Adolescent Health assessed twice during adolescence and then re-interviewed 5 years later. Component 1 was tested in 18,286 subjects, component 2 in sibling pairs (n=360) discordant for marijuana use, and component 3 in a genetically informative sub-sample (n=4846). Marijuana use was defined as any use during adolescence, and drug use was defined as self-reported past year use of other illicit drugs besides marijuana. Marijuana users were twice as likely to use illicit drugs as young adults than non-users. Shared environmental factors mediated much of the relationship between adolescent marijuana use and young adult drug use. The association remained, however, even when controlling for familial environmental and other measured factors.     

The Impact of Patient Access to Their Electronic Health Record on Medication Management Safety: A Narrative Review

IntroductionAs the American’s Federal Health Insurance Portability and Accountability Act (HIPAA) stated that patients should be allowed to review their medical records, and as information technology is ever more widely used by healthcare professionals and patients, providing patients with online access to their own medical records through a patient portal is becoming increasingly popular. Previous research has been done regarding the impact on the quality and safety of patients’ care, rather than explicitly on medication safety, when providing those patients with access to their electronic health records (EHRs).AimThis narrative review aims to summarise the results from previous studies on the impact on medication management safety concepts of adult patients accessing information contained in their own EHRs.ResultA total of 24 studies were included in this review. The most two commonly studied measures of safety in medication management were: (a) medication adherence and (b) patient-reported experience. Other measures, such as: discrepancies, medication errors, appropriateness and Adverse Drug Events (ADEs) were the least studied.ConclusionThe results suggest that providing patients with access to their EHRs can improve medication management safety. Patients pointed out improvements to the safety of their medications and perceived stronger medication control. The data from these studies lay the foundation for future research.