胎肝细胞质液对重症病毒性肝炎患者外周血TL－CFU及mIL－2R影响的实验观察

采用半固体一步单层琼脂培养法和单克隆荧光抗体技术分别观察重症肝炎外周血ＴＬ－ＣＦＵ和ｍＩＬ－２Ｒ，发现重症肝炎患者ＴＬ－ＣＦＵ（１０４．４±３２．６）及ｍＩＬ－２Ｒ（３５．６±８．６）较正常人明显降低。在培养体系中加胎肝细胞质液后，无论在重症肝炎组还是在正常组均不能明显地提高ＴＬ－ＣＦＵ，说明胎肝细胞质液不含具生物佐的促ＴＬ－ＣＦＵ因子。但对ｍＩＬ－２Ｒ表达的影响，在重症肝炎组病人，只有在ＰＨＡ存在条件下才能促进ｍＩＬ－２Ｒ的表达，说明胎肝细胞质波含有某种（些）物质能协同ＰＨＡ促进重症肝炎患者外周血淋巴细胞ｍＩＬ－２Ｒ表达。     

Evaluation of biofilm formation ability in different Candida strains and anti-biofilm effects of Fe3O4-NPs compared with Fluconazole: an in vitro study

Objective

Biofilm formation ability is one of the major virulence factors contributing to the pathogenesis of Candida species. Biofilms produced by Candida spp. cause complicated treatments and contribute to increasing unpleasant mortality rates. Nanoparticles of Fe₃O₄ (Fe₃O₄-NPs) are considered due to their magnetic and biochemical properties, as well as their low costs. The purpose of present study was to determine biofilm formation ability in different Candida strains and evaluation of anti-biofilm effect of Fe₃O₄-NPs compared with FLC.

Antigen-specific free immunoglobulin light-chain antibodies: could it be a new diagnostic marker for patients with allergy?

OBJECTIVES: Human free immunoglobulin light chains (FLCs) have long been considered as nonmeaningful spillover remnants from the process of immunoglobulin production; however, recent findings suggest that the antibody activity of FLCs may be involved in the pathology of allergic responses. We therefore assessed the antigen-binding ability of FLCs to evaluate their usefulness as diagnostic markers for patients with allergy. DESIGN AND METHODS: FLCs were separated from the serum samples of patients seropositive against cedar pollen and mice immunized with bovine serum albumin and 1-fluoro-2,4-dinitrobenzene by ultrafiltration and protein G absorption. A sensitive immunoassay confirmed the absence of any IgG in the separated FLC fractions from the human serum samples. RESULTS: Solid-phase immunoassay for cedar pollen showed that none of the human serum samples possessed any antibody activity against the antigen after the removal of whole immunoglobulins. Furthermore, while the immunized mice also showed high antibody titers against the antigens, but the serum specimens showed no residual antibody activity against the antigens after the FLCs were separated from the whole immunoglobulins. CONCLUSIONS: The results of the present study suggested that the FLC fractions may possess little or no antigen-binding activity, and that therefore, they may not serve as useful diagnostic markers in patients with allergy.     

皱皮木瓜总黄酮松弛胃肠平滑肌的效应机制分析

目的:探讨皱皮木瓜总黄酮(FLC)松弛胃肠平滑肌的机制及其与Ca2 的关系。方法:采用家兔离体胃底、回肠和结肠带生物测定法,观察FLC对不同激动剂(Ach和CaCl2)的反应;用原子吸收分光光度仪测定FLC对游离Ca2 的影响。结果:FLC产生剂量依赖性抑制Ach和CaCl2所致家兔胃底、回肠肌条的收缩,非竞争性拮抗Ach和CaCl2累积量效曲线,最大反应压低,FLC和Ver能抑制Ach诱导结肠带依内钙性收缩,对依外Ca2 收缩,仅高浓度(0.4%)FLC亦有抑制作用(P<0.05),Ver则无影响.此外,FLC具有一定的Ca2 络合作用。结论:FLC松弛胃肠平滑肌与阻断VDC和ROC,减少外Ca2 内流和内Ca2 释放有关。     

UFLC法测定四季抗病毒合剂中4种成分的质量浓度

目的测定四季抗病毒合剂中绿原酸、1,3-二咖啡酰奎宁酸、木犀草苷和3,5-二咖啡酰奎宁酸的质量浓度。方法采用超快速液相色谱(UFLC)法。色谱条件:色谱柱为Kromasil C18色谱柱(250mm×4.6mm,5μm);流动相为乙腈-3.0g·L~(-1)磷酸溶液梯度洗脱;流速为0.8mL·min~(-1);检测波长为348nm;柱温为35℃。结果绿原酸、1,3-二咖啡酰奎宁酸、木犀草苷和3,5-二咖啡酰奎宁酸的线性范围分别为24.00~960.00,22.00~880.00,3.00~120.00和24.00~960.00μg·mL~(-1),相关系数分别为0.999 3,0.999 2,0.999 6和0.999 3;平均回收率分别为98.62%(RSD=1.16%),98.90%(RSD=1.67%),98.37%(RSD=0.95%)和98.35%(RSD=0.91%);12批制剂中绿原酸、1,3-二咖啡酰奎宁酸、木犀草苷和3,5-二咖啡酰奎宁酸的平均质量浓度分别为142.91,191.39,16.89和140.20μg·mL~(-1),RSD值分别为0.97%,1.05%,1.16%和1.20%。结论文章测定的4种成分及建立的方法,可用于四季抗病毒合剂的质量评价。     

用人胎肝细胞重建SCID鼠人免疫系统

用人胎肝细胞重建重症联合免疫缺陷鼠(SCID)人免疫系统(SCID-hu)的初步结果。通过免疫组织化学及流式细胞术检测,腹腔内注射胎肝细胞第2周,SCID鼠脾内即可见人淋巴细胞,至第10周仍可测到CD20~ 、CD19~ 、CD3~ 人淋巴细胞;用HBx蛋白重复免疫SCID-hu鼠后第2周,外周血中可测到人源抗HBx抗体(IgG)。提示:人干细胞可在SCID鼠体内存活、分化、成熟,并能对抗原产生特异性的初次及再次免疫反应,腹腔内注射人胎肝细胞是一种较简便、实用的重建SCID-hu的方法,并可用于人源单抗、肿瘤及HIV等病毒感染性疾病的研究;SCID-hu可望解决人源单抗制备中B细胞来源困难的问题。     

Serum immunoglobulin free light chain levels in systemic autoimmune rheumatic diseases

Several reports have highlighted the abnormal increments of serum immunoglobulin free light chains (FLCs) in the course of systemic autoimmune rheumatic diseases (SARD), but a comparative analysis among different conditions is still lacking. A strong association between elevated FLC and hepatitis C virus (HCV)-related mixed cryoglobulinaemia (HCVMC) has been well established. Here, we aimed to analyse serum FLC levels in patients with four different SARD in comparison with HCVMC. Using a turbidimetric assay, free κ and λ chains were quantified in sera from 198 SARD patients (37 rheumatoid arthritis, RA; 47 systemic lupus erythematosus, SLE; 52 anti-phospholipid syndrome, APS; 62 primary Sjogren's syndrome, pSS), 62 HCVMC and 50 healthy blood donors (HD). All patient groups showed increased κ levels when compared to HD: 33·5 ± 2·6 mg/l in HCVMC, 26·7 ± 2·3 mg/l in RA, 29·7 ± 1·9 mg/l in SLE, 23·8 ± 1·1 mg/l in APS, 24·2 ± 1·1 mg/l in pSS; 10·1 ± 0·6 mg/l in HD. Free λ levels displayed a significant increase only for HCVMC (20·4 ± 1·4 mg/l) and SLE (18·4 ± 1·0 mg/l) compared to HD (13·6 ± 0·9 mg/l). The increase of κ compared to λ takes into account a κ /λ ratio of 1·6 for all groups. Our results substantially analyse and strengthen the association between FLC and SARD focusing the questions regarding their role in the pathogenesis and diagnosis of human diseases. Unfortunately, the biochemical differences distinguishing normal from pathological FLC have not been identified. Production of different isotypes is probably connected to still-unknown pathways.     

PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin is required for the vernalization-responsive, winter-annual habit in Arabidopsis

The winter-annual habit (which typically involves a requirement for exposure to the cold of winter to flower in the spring) in Arabidopsis thaliana is mainly due to the repression of flowering by relatively high levels of FLC expression. Exposure to prolonged cold attenuates FLC expression through a process known as vernalization and thus permits flowering to occur in the spring. Here we show that the elevated FLC expression characteristic of nonvernalized winter annuals requires two genes, EARLY FLOWERING 7 (ELF7) and EARLY FLOWERING 8 (ELF8), that are homologs of components of the PAF1 complex of Saccharomyces cerevisiae. Furthermore, ELF7 and ELF8 are also required for the expression of other genes in the FLC clade of flowering repressors such as MAF2 and FLM. FLC, FLM, and MAF2 are involved in multiple flowering pathways that account for the broad effects of elf7 and elf8 mutations on flowering behavior. ELF7 and ELF8 are required for the enhancement of histone 3 trimethylation at Lys 4 in FLC chromatin. This modification of FLC chromatin appears to be required to elevate FLC expression to levels that can delay flowering in plants that have not been vernalized. A model of the role of ELF7, ELF8, and other previously described genes in the modification of the chromatin of flowering repressors is presented.