不同针刺手法对健康人经穴氧分压的影响

本文采用氧分压（ＰＯ２）传感针，对２０例健康男性的手阳明大肠经上臂穴、曲池穴的ＰＯ２与相应非穴位的ＰＯ２值进行了测定。结果显示针刺合谷穴时，臂与曲池穴ＰＯ２升高，分别与针刺前比较，其１０＇、１５＇、２０＇的ＰＯ２值均有显著性差异（ＰＭ＜Ｏ．０５），而相应非穴位ＰＯ２变化不显著。在应用补法与泻法（提插捻转）运针后发现，施行补法时曲池穴Ｐ０２上升，泻法时ＰＯ２下降。     

Effects of D3.49A,R3.50A,and A6.34E mutations on ligand binding and activation of the cannabinoid-2 (CB2) receptor

In several G protein-coupled receptors (GPCRs), the Asp-Arg-Tyr (DRY) motif at the bottom of third transmembrane domain and the amino acid at position 6.34 in the sixth transmembrane domain have been shown to play important roles in signal transduction. In this study, we propose that in the cannabinoid-2 (CB2) receptor, R3.50 in the DRY motif may be crucial for interacting with G proteins, and D3.49 and A6.34 may be important for constraining the receptor in an inactive conformation. To test our hypothesis, R3.50A, D3.49A, and A6.34E mutations of the human CB2 receptor were made by site-directed mutagenesis. These mutant receptors were stably transfected into human embryonic 293 cells, and their ligand binding and signal transduction properties were analyzed. Similar to other GPCRs, R3.50 of the CB2 receptor is crucial for signal transduction. Unlike other GPCRs, D3.49 and A6.34 of the CB2 receptor do not seem to be important for keeping the receptor in an inactive state. Furthermore, D3.49A and A6.34E mutations abolished ligand binding, and all three mutations abolished constitutive activity of the wild-type CB2 receptor.     

Reinforcing Gastric Staple-Lines with Bovine Pericardial Strips May Decrease the Likelihood of Gastric Leak after Laparoscopic Roux-en-Y Gastric Bypass

Background: Staple-line leakage is a potentially devastating complication of Roux-en-Y gastric bypass (RYGBP). Bovine pericardial strips (BPS) have been used to reinforce staple-lines in pulmonary resections and have been shown to decrease air-leaks.This study examined the use of BPS to decrease gastric staple-line leaks. Methods: 250 consecutive patients undergoing laparoscopic RYGBP had BPS used for the gastric pouch. Ease of use, operative complications, and visual bleeding were recorded. In addition, the postoperative leak rate was compared to 100 consecutive patients operated on prior to the study when BPS was not used. Results: Patient characteristics were the same for both groups and comparable with most published data. With the use of BPS, there were no operative complications and no meaningful increase in operating time. BPS reinforced staple-lines had no visual bleeding. In the 100 cases without BPS, there were 2 staple-line leaks (2%) both of which required emergency exploration. One additional patient was subsequently found to have a gastrogastric fistula. In the 250 patients who had BPS-reinforced staple-lines, there were no acute leaks.Three patients were subsequently found to have gastrogastric fistula. None required emergency surgery. Conclusion: In this non-randomized trial, BPS were found to be easy and safe to use. In addition, staple-line hemorrhage was essentially non-existent. Although the reduction in gastric staple-line leak rate may also be attributed to learning curve, there were no acute leaks in 250 patients with BPS, which is below the published norms.     

东莞地区2000例荨麻疹患者变应原分析

【目的】分析和研究东莞地区2000例荨麻疹患者变应原的分布情况。【方法】选取荨麻疹患者共2000例,采用标准测量方法“阿罗格”点刺原液进行变应原测试,分析其变应原结果。【结果】2000例荨麻疹患者变应原以粉尘螨、蟑螂、屋尘螨最高,其次为小虾、牛肉、蚌类。【结论】尘螨、蟑螂、屋尘螨等是东莞地区荨麻疹患者中最常见的变应原。     

Computational Analysis of the 3-D structure of Human GPR87 Protein: Implications for Structure-Based Drug Design

The G-protein coupled receptor 87 (GPR87) is a recently discovered orphan GPCR which means that thesearch of their endogenous ligands has been a novel challenge. GPR87 has been shown to be overexpressed insquamous cell carcinomas (SCCs) or adenocarcinomas in lungs and bladder. The 3D structure of GPR87 washere modeled using two templates (2VT4 and 2ZIY) by a threading method. Functional assignment of GPR87by SVM revealed that along with transporter activity, various novel functions were predicted. The 3D structurewas further validated by comparison with structural features of the templates through Verify-3D, ProSA andERRAT for determining correct stereochemical parameters. The resulting model was evaluated by Ramachandranplot and good 3D structure compatibility was evidenced by DOPE score. Molecular dynamics simulation andsolvation of protein were studied through explicit spherical boundaries with a harmonic restraint membranewater system. A DRY-motif (Asp-Arg-Tyr sequence) was found at the end of transmembrane helix3, whereGPCR binds and thus activation of signals is transduced. In a search for better inhibitors of GPR87, in silicomodification of some substrate ligands was carried out to form polar interactions with A     

经穴皮部挑治与深刺的fMRI对比研究

为证实皮部挑治与经穴深刺同样有效，采用ｆＭＲＩ（核磁共振脑功能成像技术）进行实验观察。经过３２例次的实验，结果证明：两种方法均可在同一脑区产生功能变化和相应的变化过程。这一结果证实经络皮部的客观存在，经络具有内病外治的作用，并为经络客观化研究提供新途径。     

化浊益智针法治疗血管性痴呆的临床疗效观察及机理研究

对５０例血管性痴呆（ＶＡＤ）患者进行了临床疗效及相关理化指标的临床研究，并与传统针法进行了对比观察。结果表明，两种针法均可提高ＶＡＤ患者的智能（ＨＤＳ）与社会功能活动（ＦＡＱ）（Ｐ＜０．００１），改善颅内血流动力学状态及血流变的高粘、高凝、高聚状态，提高大脑皮层兴奋性。化浊益智针法在临床总有效率、ＨＤＳ与ＦＡＱ的积分改善、在延长脑血流流入时间、降低全血粘度、红细胞压积与血小板最大聚集率（ＭＡＲ）方面，均较传统针法显著（Ｐ＜０．０５）。     

七星针为主治疗小儿脑性瘫痪260例疗效观察

七星针为主治疗小儿脑性瘫痪２６０例疗效观察袁清顺（北京市朝阳中医医院，１０００２０）主题词脑性麻痹／针灸疗法；肌痉挛／针灸疗法；运动障碍／针灸疗法；刺法Ｔｒｅａｔｍｅｎｔｏｆ２６０ＣａｓｅｓｏｆＩｎｆａｎｔｉｌｅＣｅｒｅｂｒａｌＰａｒａｌｙｓｉｓｂｙ...     

A Comparison of Burst Pressure Between Buttressed Versus Non-Buttressed Staple-Lines in an Animal Model

Background: The consequences of a staple-line leak or disruption can be devastating particularly in the bariatric surgery population. This study was designed to assess if gastrointestinal staple-lines buttressed with bovine pericardium could tolerate greater intraluminal pressures compared with non-reinforced staple-lines. Methods: A laparoscopic linear cutting staple device was used to create divided staple-lines across the small intestine in a porcine model and the stomach in a rabbit model. 21 staple-lines were created with buttressing strips of bovine pericardium, and another 21 staple-lines were created without any reinforcement. All staple-lines were subjected to a constant rate increase in intraluminal pressure via intraluminal infusion of a dye solution. At the first sign of seam leak or failure, the burst pressure was recorded. Results: In 19 of the 21 studies, the staple-lines buttressed with bovine pericardium sustained higher mean burst pressures than the conventional non-buttressed staple-lines. For the porcine intestinal segments, the mean intraluminal pressure at failure was 125 ± 24 mmHg for buttressed staple-lines versus 58.4 ± 28mm Hg for conventional staple-lines (P <0.0001). For rabbit stomach segments, the mean intraluminal pressure at failure was also significantly higher for the buttressed segments (115 ± 24 vs 75.6 ± 23, P <0.0137). Most buttressed segments failed away from the staple-line, while the non-buttressed segments failed at the staple-line. Conclusions: Gastrointestinal staple-lines buttressed with bovine pericardium are able to maintain seam integrity at significantly higher intraluminal pressures when compared to non-buttressed GI staple-lines in animal models.     

Characterization of functional roles of DRY motif in the 2nd intracellular loop of dopamine D2 and D3 receptors

Dopamine D₂R and D₃R (D₂R, D₃R) show very high sequence homology and employ virtually identical signaling pathways even though D₂R is 2 ∼ 5 times more active. Among the structural motifs identified, a triplet sequence, Asp-Arg-Tyr (DRY motif), plays critical roles in the determination of receptor conformations for signaling and intracellular trafficking of G protein-coupled receptors by forming intramolecular interactions. Thus, it is possible that different signaling efficiencies of D₂R and D₃R might be caused by the receptor activation levels stabilized by their own DRY motifs. In this study, the Arg and Asp residues of D₂R and D₃R were mutated, and resulting changes in their signaling and intracellular trafficking properties were comparatively studied. Mutation of the Arg residues of D₂R and D₃R abolished their signaling but differently affected their intracellular localizations. The wildtype and R132H-D₂R were expressed mainly on the plasma membrane. On the other hand, compared with the wildtype D₃R, a substantial amount of R128H-D₃R was localized intracellularly. The expression of receptor proteins on the plasma membrane and their signaling efficiencies were more drastically affected by the mutation of the Asp residue of D₃R than D₂R. Therefore, it was concluded that the different levels of conformational strain exerted by the DRY motif might partly determine the quantitative differences in the signaling efficiencies between D₂R and D₃R. These authors contributed equally to this work.