Plasminogen activation in plasma of patients with systemic lupus erythematosus

Summary We measured ₂-plasmin inhibitor-plasmin complexes (PI-PC) in plasma of patients with systemic lupus erythematosus (SLE) to examine the plasminogen activation in SLE. The plasma PI-PC level in 23 patients with SLE was significantly higher than that in 18 normal subjects (P<0.001) and the SLE patients with nephrotic syndrome had higher plasma PI-PC levels than those without nephrotic syndrome (P<0.01). In addition, the plasma PI-PC level was significantly correlated with the level of plasma C3 breakdown products (iC3b/C3dg) in the patients with SLE (r=0.53, P<0.01). These results suggest that plasminogen is activated in plasma of patients with SLE and that the plasminogen activation may be associated with the activation of complement in SLE.     

Total hemolytic complement (CH50) and its fractions (C3 and C4) in the sera of patients with carcinoma of the oral cavity,uterine cervix,and breast

The total hemolytic complement activity of CH50 and its fractions C3 and C4 was determined in the sera of 196 patients with carcinoma of the oral cavity, 172 patients with carcinoma of the uterine cervix, and 166 patients with breast cancer. The values were compared with those of 18 patients with mammary dysplasia, 32 patients with mild to moderate dysplasia of the cervix, and 100 healthy, normal age- and sex-matched controls. No alterations in CH50, C3, and C4 were observed in the sera of patients with benign lesions, whereas a significant rise in the three factors was observed in all the cancer patients studied. The complement activity increased significantly with the progression of the disease up to stage III and remained persistently elevated thereafter. Patients who had a clinical cure had normal levels of CH50, C3, and C4, whereas the values remained elevated in patients who were still undergoing treatment for residual lesions.     

补体通过激活足细胞ERK信号通路上调PD-L1表达

目的:探讨补体损害足细胞的可能机制。方法:将足细胞分为对照组、补体刺激组、补体+ERK抑制剂组,免疫蛋白印迹法检测足细胞PD-L1、细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)表达,并用细胞迁移实验检测足细胞功能。结果:补体刺激组足细胞ERK/p-ERK表达上调(P<0.05),PD-L1表达上调(P<0.05),加入ERK抑制剂后足细胞PD-L1表达下调,伴迁移减少。结论:补体通过激活ERK/p-ERK信号通路上调PD-L1表达,导致足细胞损害。     

丙型肝炎患者HCV-RNA载量与免疫球蛋白及补体表达水平的相关性

目的 探讨丙型肝炎患者HCV-RNA载量与免疫球蛋白及补体表达水平的相关性.方法 收集2014年6月至2016年5月西安市第九医院收治的丙型肝炎患者132例作为研究对象,对其进行HCV-RNA病毒载量检测,按照检测结果将其分为低载量、中载量以及高载量组,对各组进行免疫球蛋白IgA、IgG、IgM检测以及补体C3、C4检测,分析丙肝患者病毒载量与免疫球蛋白及补体水平的相关性.结果 丙型肝炎患者IgA、IgG、IgM表达量分别为[(3.95±0.72) g/L,(19.33±4.64) g/L及(2.93±0.50)g/L],高于健康对照组的表达量[(2.13 ±0.51) g/L,(11.25±2.63) g/L及(1.21±0.35) g/L],C3、C4表达量分别为[(0.82±0.19) g/L及(0.13±0.08)g/L],低于健康对照组[(1.20±0.31)g/L及(0.27±0.11)g/L],差异有统计学意义(P＜0.05).不同病毒载量患者免疫球蛋白及补体表达水平存在差异(P＜0.05).Pearson相关性分析结果显示,HCV-RNA病毒载量与血清免疫球蛋白表达水平呈正相关,与血清补体表达水平呈负相关(P＜0.05).结论 不同病毒载量丙型肝炎患者具有不同的免疫球蛋白及补体表达水平,应当尽量控制HCV-RNA病毒载量,从而减少免疫损伤.     

Small Intestinal Bacterial Overgrowth versus Antimicrobial Capacity in Patients with Spontaneous Bacterial Peritonitis

Background: Spontaneous bacterial peritonitis (SBP) is a serious infection in cirrhotic patients with ascites. Both defects in the host defense mechanisms and the enhancement of the offensive factor (small intestinal bacterial overgrowth (SIBO)) may contribute to the development of SBP. Therefore, the aim of this study was to evaluate the role of SIBO versus various antimicrobial capacities in the pathogenesis of SBP in cirrhotic patients. Methods: Forty-five cirrhotic patients were enrolled in this study. Bacterial overgrowth was evaluated by breath hydrogen test (BH₂T). The hepatic reticuloendothelial system phagocytic index (HRESPI) was measured by intravenously injected colloid suspensions. Results:     

清肺化瘀散对老年下呼吸道感染患者白细胞介素6、白细胞介素8、超敏C反应蛋白及补体C_3、C_4的影响

[目的]观察清肺化瘀散对老年下呼吸道感染患者白细胞介素6、白细胞介素8、超敏C反应蛋白及补体C3、C4的影响。[方法]将60例老年下呼吸道感染患者随机分为治疗组与对照组。对照组经验性予以抗生素治疗,治疗组在对照组基础上予中药汤剂清肺化瘀散治疗,疗程均为7~14天,观察两组的疗效和治疗前后白细胞介素6(IL-6)、白细胞介素8(IL-8)、超敏C反应蛋白(hs-CRP)及补体C3、C4的变化。[结果]治疗组在改善临床症状、体征,促进实验室检查等回复正常,改善中医症候学方面明显优于对照组(P<0.05);治疗组治疗后IL-6、IL-8、hs-CRP、补体C3下降水平优于对照组(P<0.05)。[结论]清肺化瘀散配合西医治疗老年人下呼吸道感染疗效优于常规西医治疗。     

Study on the immunological safety of universal plasma in the Chinese population in vitro

Background

The prepared procedure for universal plasma in the Chinese population has been developed. However, the immunological safety with the level of antibodies, soluble immune complexes and complements is necessary to investigate.

血清补体C3、C4在颅脑损伤中的临床意义

目的分析颅脑损伤患者血清补体C3、C4的动态变化与患者病情及预后的关系。方法抽取65例颅脑损伤患者伤后第1、3、7、14天的静脉血,检测其血清中补体C3、C4的浓度,根据入院时GCS评分将患者分为轻、中、重型三组,再按出院时GOS评分将患者分为预后良好、预后不良和死亡三组,分析补体C3、C4的浓度与病情、预后之间的关系。结果中型和重型颅脑损伤患者、预后不良和死亡患者血清中补体C3、C4在伤后第3、7天显著升高(P0.05),第14天降至正常;中、重型和轻型组以及预后不良、死亡组和预后良好组之间在伤后第3、7天血清补体C3、C4浓度差异显著(P0.05),且和GCS、GOS评分有明显相关性(P0.05)。结论颅脑损伤后血清C3、C4过度升高与病情及预后相关,C3、C4的升高提示患者病情较重,预后较差。     

Low-dose oral methotrexate for the management of childhood Cogan’s syndrome: a case report

Cogan’s syndrome is a rare inflammatory disease characterized by nonsyphilitic ocular interstitial keratitis associated with hearing loss and vestibular impairment. Although systemic corticosteroids are usually used as the standard therapy, hearing ability in most cases gradually deteriorates. We, herein, describe a patient with childhood Cogan’s syndrome who was treated with low-dose oral methotrexate, which successfully helped to taper the doses of the systemic corticosteroids. The serum levels of the complements were good markers for the disease activity in this patient.     

血清PAB、AAG、C_3、C_4的检测在肝病诊断中的意义

目的探讨血清前白蛋白(PAB)α1-酸性糖蛋白(AAG)及补体C3、C4在肝脏疾病诊断中的临床意义。方法采用免疫散射比浊法测定各种类型肝病患者血清中的PAB、AAG、补体C3、C4的含量,并与正常对照组比较。结果各型肝病中的PAB都有不同程度的下降,急性肝炎和肝癌中的AAG明显增高,慢性肝炎、肝硬化及重肝中的AAG明显下降。急性肝炎及肝癌的C3、C4明显升高。其他各型肝病均有不同程度的下降。结论PAB、AAG、C3、C4联合检测对肝脏疾病有非常重要的诊断价值。