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排序方式: 共有277条查询结果,搜索用时 15 毫秒
1.
In 1396 consecutively patch tested patients 18 (1.3%) reacted to Kathon CG. Relevance was established in 4 of the 18 patients. The frequency of positive reactions to Kathon CG in eczema patients seems to have been stable in Denmark during the period 1983 to 1988. 相似文献
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I. Virgolini P. Fitscha H. Sinzinger H. Barth 《European journal of clinical pharmacology》1990,38(4):347-350
Summary In order to assess the effect of taprostene on haemodynamics, platelet function and arachidonate metabolism in 4 healthy volunteers an intravenous infusion of 25 ng · kg–1 · min–1 was given for 6 h.During the infusion period systolic blood pressure dropped from 130 to 111 mm Hg and diastolic blood pressure from 77 to 69 mm Hg. The heart rate rose from 77 to 84 beats/min.During the taprostene infusion the slope and height of the ADP and collagen induced platelet aggregation curves were significantly inhibited and the sensitivity of platelets to PGI2 and PGE1 was increased.Plasma and serum thromboxane B2, conversion of exogenous radiolabelled arachidonic acid, WU-test, circulating endothelial cell count, concentration of platelet factor 4, -thromboglobulin, malondialdehyde and the PGI2-synthesis stimulating plasma factor did not show any clear drug-related alteration.It is concluded that infusion of taprostene 25 ng · kg–1 · min–1 caused measurable inhibition of platelet function ex vivo. 相似文献
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Nobuhiko Tachibana Yoko Yamashita Mayuko Nagata Satoshi Wanezaki Hitoshi Ashida Fumihiko Horio Mitsutaka Kohno 《Nutrition Research》2014
Although the underlying mechanism is unclear, β-conglycinin (βCG), the major component of soy proteins, regulates blood glucose levels. Here, we hypothesized that consumption of βCG would normalize blood glucose levels by ameliorating insulin resistance and stimulating glucose uptake in skeletal muscles. To test our hypothesis, we investigated the antidiabetic action of βCG in spontaneously diabetic Goto-Kakizaki (GK) rats. Our results revealed that plasma adiponectin levels and adiponectin receptor 1 messenger RNA expression in skeletal muscle were higher in βCG-fed rats than in casein-fed rats. Phosphorylation of adenosine monophosphate–activated protein kinase (AMP kinase) but not phosphatidylinositol-3 kinase was activated in βCG-fed GK rats. Subsequently, βCG increased translocation of glucose transporter 4 to the plasma membrane. Unlike the results in skeletal muscle, the increase in adiponectin receptor 1 did not lead to AMP kinase activation in the liver of βCG-fed rats. The down-regulation of sterol regulatory element-binding factor 1, which is induced by low insulin levels, promoted the increase in hepatic insulin receptor substrate 2 expression. Based on these findings, we concluded that consumption of soy βCG improves glucose uptake in skeletal muscle via AMP kinase activation and ameliorates hepatic insulin resistance and that these actions may help normalize blood glucose levels in GK rats. 相似文献
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Menon KN Ikeda T Fujimoto I Narimatsu H Nakakita S Hase S Ikenaka K 《Journal of neuroscience research》2005,80(1):29-36
Oligosaccharides with biantennae and bisecting N-acetyl glucosamine (GlcNAc) residues attached to the mannose in the beta1-4 trimannosyl core (BA2) are enriched in the brain and considered brain-type sugar chains. We investigated the significance of the interplay between galactosyltransferase I (GalTase I) and BA2 formation in a brain-derived cell line, CG4. Increased GalTase expression in different glial- and neuronal-derived cell lines was accompanied by decreased or undetectable levels of BA2, depending on the level of GalTase expression. Forceful expression of GalTase I in CG4 cells expressing high levels of BA2 and low GalTase activity significantly reduced BA2 levels. In addition, a sixfold increase in an abnormal sugar chain A1(6)G1Fo and a moderate increase in A2G2Fo(6)F were evident. The increased levels of A1(6)G1Fo indicate a diversion or abrogation of the N-linked sugar chain biosynthetic pathway from normal. The accumulation of A1(6)G1Fo and increased A2G2Fo(6)F levels were accompanied by decreased levels of the high mannose-type sugar chains, M5A, M6B, M8A, and M9A. Increased GalTase I expression also led to stunted growth and abnormal morphology of CG4 cells, with increased mortality. Even moderate overexpression of GalTase I thus disrupts the normal biosynthetic pathway of N-linked sugar chains, and high overexpression is fatal to CG4 cells. 相似文献
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Lacerra G Fiorito M Musollino G Di Noce F Esposito M Nigro V Gaudiano C Carestia C 《Human mutation》2004,24(4):338-349
The alpha-globin chains are encoded by two duplicated genes (HBA2 and HBA1, 5'-3') showing overall sequence homology >96% and average CG content >60%. alpha-Thalassemia, the most prevalent worldwide autosomal recessive disorder, is a hereditary anemia caused by sequence variations of these genes in about 25% of carriers. We evaluated the overall sensitivity and suitability of DHPLC and DG-DGGE in scanning both the alpha-globin genes by carrying out a retrospective analysis of 19 variant alleles in 29 genotypes. The HBA2 alleles c.1A>G, c.79G>A, and c.281T>G, and the HBA1 allele c.475C>A were new. Three pathogenic sequence variations were associated in cis with nonpathogenic variations in all families studied; they were the HBA2 variation c.2T>C associated with c.-24C>G, and the HBA2 variations c.391G>C and c.427T>C, both associated with c.565G>A. We set up original experimental conditions for DHPLC and DG-DGGE and analyzed 10 normal subjects, 46 heterozygotes, seven homozygotes, seven compound heterozygotes, and six compound heterozygotes for a hybrid gene. Both the methodologies gave reproducible results and no false-positive was detected. DHPLC showed 100% sensitivity and DG-DGGE nearly 90%. About 100% of the sequence from the cap site to the polyA addition site could be scanned by DHPLC, about 87% by DG-DGGE. It is noteworthy that the three most common pathogenic sequence variations (HBA2 alleles c.2T>C, c.95+2_95+6del, and c.523A>G) were unambiguously detected by both the methodologies. Genotype diagnosis must be confirmed with PCR sequencing of single amplicons or with an allele-specific method. This study can be helpful for scanning genes with high CG content and offers a model suitable for duplicated genes with high homology. 相似文献
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Leslie Lobel Susan Pollak Sujing Wang Michael Chaney Joyce W. Lustbader 《Endocrine》1999,10(3):261-270
We have linked two human chrionic gonadotropin (hCG) β-subunit cDNAs in tandem such that the expressed fusion protein consists
of two mature β-subunits joined through the carboxy terminal peptide of the first β-subunit. A single glycine residue is inserted
between the two subunits in the fusion protein. Chinese hamster ovary (CHO) cells transformed with a clone that contains the
fused cDNAs express and secrete a protein that is consistent with it being a β-hCG homodimer protein. These β-homodimer molecules
can recombine with two free α-subunits indicating that both β-subunits within the homodimer are likely folded in their native
conformation. Our data also suggest that the two β-subunits fold upon each other as a globular protein and do not appear to
exist as a simple fusion of two linear β-subunits. Further-more, the two β-monomer subunits in the fusion protein form a stable
homodimer that can bind and activate the hLH/CG receptor specifically. Recombination of the fusion protein with α-subunits
appears to favor an arrangement where two α-subunits combine with a single molecule of the fusion protein. The recombined
molecule consists of four subunits and is comparable to two tethered hCG moieties, which constitutes a hCG dimer. This hormone
dimer can bind and activate the hLH/CG receptor with an activity approximating that of native hCG. 相似文献
10.
不同孕期妇女血清CG和FE3检测的临床意义 总被引:2,自引:0,他引:2
目的:探讨了不同孕期妇女血清甘胆酸(CG)和游离雌三醇(FE3)水平的变化及意义。方法:应用放射免疫分析对102例不同孕期的妇女进行了血清CG、FE3检测,并与35名正常非孕妇女进行比较。结果:不同孕期妇女血清CG和FE3水平均显著升高与正常非孕妇女血清比较有显著性差异(P<0.05~0.01)。结论:检测不同孕期妇女血清CG和FE3水平的变化对了解病情、指导临床实践均有重要的临床价值。 相似文献