派丽奥软膏治疗急性局限型下颌智齿冠周炎的临床观察

目的观察派丽奥软膏(periocline ointment,盐酸米诺环素软膏)治疗急性局限型下颌智齿冠周炎的临床效果及不良反应。方法用随机、单盲法研究派丽奥软膏于盲袋中置药的治疗效果,与碘甘油对照,第4天观察临床效果及不良反应。结果派丽奥软膏治疗组,临床治愈率46.67%,高于碘甘油组(χ2=6.435,P<0.05);显效率81.67%,差异有显著性(χ2=9.440,P<0.01)。未发现不良反应。结论派丽奥软膏治疗急性局限型下颌智齿冠周炎有良好的临床效果。     

大剂量丙种球蛋白治疗小儿全身炎症反应综合征临床研究

目的　观察静脉注射丙种球蛋白 (IVIG)对小儿全身炎症反应综合征 (SIRS)免疫功能的影响及临床疗效。方法　对符合诊断标准的 76例SIRS患儿随机分为治疗组 (39例 )和对照组 (37例 ) ,在基础治疗相同的情况下 ,治疗组给予IVIG按每次 40 0～ 10 0 0mg/kg ,每日 1次 ,连用 3d ,并于治疗前后测定Ig、CD4 、CD8、CD4 /CD8的变化。结果　IVIG治疗前后相比 ,IgG、CD4 、CD8及CD4 /CD8明显升高 (P <0 .0 5 )。与对照组相比 ,IVIG组疗效显著 (χ2 =7.5 2 ,P <0 .0 1) ,且无明显不良反应发生。结论　大剂量IVIG可调节SIRS患儿的免疫机制并改善其预后。     

盐酸米诺环素牙周条治疗牙周炎的临床研究

目的：评价牙周局部应用盐酸米诺环素牙周条辅助治疗牙周炎的临床效果。方法：选择成人慢性牙周炎患者42例，左右侧对应牙，牙周袋深度≥4mm,且探诊出血的牙位占252个，采用随机单自对照法，分为实验组和对照组，基础治疗后实验组牙周袋内放药，每周1次，共3次，对照组不放药。于基线、治疗后第6周、第12周时，观察菌斑指数（PLI）、牙周袋深度（PD）、探诊后出血（BOP）等指标。结果：治疗后第6周和第12周时，两组各观察指标比基线时有明显的改善（P＜0.01）。两次复查时，实验组改善均优于对照组，两组相比具有显著性差异（P＜0.01）。结论：局部应用盐酸米诺环素牙周条治疗牙周炎，能够有效地改善临床症状，获得较好的临床效果。     

米诺环素局部用药治疗老年慢性牙周炎的疗效观察

目的：评价盐酸米诺环素软膏局部应用，治疗老年慢性牙周炎的疗效。0方法：选择60例老年慢性牙周炎的患者，共120颗牙。全口洁治和根面平整后，以患者一侧忠牙局部应用盐酸米诺环素软膏为实验组，对侧同名患牙局部应用双氧水、碘甘油作为对照组。于基线、基线后5、9周检测菌斑指数（PLI）、龈沟出血指数（SBI）、探诊深度（PD）。结果：两组临床指标均较基线有明显改善，PLI实验组优于对照组，差异有显著性（P〈0．01）。结论；牙周袋内应用米诺环素软膏配合牙周基础冶疗，能够有效改善牙周炎症状，且效果优于传统的碘氧疗法。     

In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCI

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated ammo-acids. Increasing concentrations of minocycline (10, 50, 100μg/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100μg/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 μg/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 fig/ml of minocyline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10μg/ml minocycline-HCl solution increased significantly (p<0.005) cell migation towards a gradient of fetal calf serum. The presence of 10 μg/ml of minocycline in contact with the kerationocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemo attractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyoud50μg/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of junctional epithelium.