Compared to conventional means of quantitative drug analysis, NMR spectroscopy only provides a limited set of adjustable parameters to enhance the quality of the analysis such as pH value, temperature, auxiliary reagents, magnetic field strength or properties of the solvent. In this work we investigate the influence of the kind of solvent on the signal separation of decisive resonances in the NMR spectra of codergocrine mesilate and flupentixol dihydrochloride. Polarity and aromaticity of the solvent play a crucial role in the optimization of signal separation. However, the set of applicable solvents is usually limited due to certain boundary conditions like solubility of the agent. Therefore the effects of solubilizers as well as mixtures solvents on the chemical shift also have to be taken into account. The quantitative results obtained by means of 1H NMR spectroscopy were found to be in good agreement with the results carried out using official HPLC methods of International Pharmacopoeias. 相似文献
A human form of a prion disorder is the Creutzfeldt-Jakob disease. A hallmark of the disease is the accumulation of misfolded prion proteins (PrPSc), which exist as heterogeneous subtypes. PrPSc is formed by protein conversion from the host-encoded cellular prion (PrPC), which is expressed and modified to various isoforms. Little is known about variation in PrPC; however, it is assumed that PrPC types play important roles in the formation of PrPSc. In this study, we separated distinct human PrPC subtypes on the basis of differential protein solubilities in detergent solutions. Single and sequential application of the detergents Triton X-100, octyl-glucopyranoside and CHAPS facilitated high solubility of glycosylated PrPC isoforms, whereas high proportions of nonglycosylated PrPC remained non-soluble. Most proteins became highly soluble with laurylsarcosine and sodium dodecyl sulphate. Our findings demonstrate that the solubility characteristics of heterogeneous PrPC overlap in human brains and convey distinct solubility subtypes. Differentiation by solubility experiments can therefore provide valuable information on prion protein composition, facilitate the separation of subtypes, and offer new prospects for conversion specificity of distinct isoforms. 相似文献
We reviewed the neuropathological changes in 12 forensic autopsies during a period of 6 months in cases with glue abuse suspicion, and observed specific macroscopic and microscopic changes in the brain with emphasis on the white matter substance.
The information of this study was taken from the investigation documents, laboratory results, and neuropathological observations.
The results were compared with the literature of neurotoxicology on solvents, especially with the examples caused by toluene and coincidences among them were described.
The results showed changes related with solvents leukoencephalopathy: multifocal alterations, diminishment, loss and fragmentation of myeline density, conservation of neural filaments and neuropile vacuolization without inflammatory changes. A characterization of the cases was made and an average age of 28.8 was presented. In Colombia, the principal solvent legally used is toluene, but it is possible that other hydrocarbons are involved. 相似文献
Summary Four volunteer subjects were exposed to 150ppm (655 mg/m3) of ethylbenzene and 150ppm (655mg/m3) of m-xylene both separately and in combination. The biotransformation of the solvents was studied on the basis of the metabolites found in the urine. The metabolic conversion of both m-xylene and ethylbenzene proceeded mainly through oxidation of side chains. Ring oxidation seemed to be of minor importance; in the case of ethylbenzene it accounted for 4.0% (combined share of 4-ethylphenol, p- and m-hydroxyacetophenones) and in case of m-xylene for 2.5% (2,4 dimethylphenol), respectively. Mandelic and phenylglyoxylic acids amounted to 90% of the ethylbenzene metabolites, whereas m-xylene were excreted to 97% in the form of m-methylhippuric acid. Almost equimolar amounts in the form of metabolites of both solvents were found in the urine during 24h from the onset of exposure. Most of the ethylbenzene metabolites were excreted at substantially slower rates than those of m-xylene. The combined exposure resulted in a mutual inhibition of the metabolism of ethylbenzene and m-xylene, which was demonstrated by delayed excretion and decreased amounts of metabolites excreted. No sign of alteration in the urinary metabolite patterns of either ethylbenzene or m-xylene could be detected. 相似文献
The present research was aimed at the enhancement of the dissolution rate of atorvastatin calcium by the solid dispersion technique using modified locust bean gum. Solid dispersions (SD) using modified locust bean gum were prepared by the modified solvent evaporation method. Other mixtures were also prepared by physical mixing, co-grinding, and the kneading method. The locust bean gum was subjected to heat for modification. The prepared solid dispersions and other mixtures were evaluated for equilibrium solubility studies, content uniformity, FTIR, DSC, XRD, in vitro drug release, and in vivo pharmacodynamic studies. The equilibrium solubility was enhanced in the solid dispersions (in a drug:polymer ratio of 1:6) and other mixtures such as the co-grinding mixture (CGM) and kneading mixture (KM). Maximum dissolution rate was observed in the solid dispersion batch SD3 (i.e. 50% within 15 min) with maximum drug release after 2 h (80%) out of all solid dispersions. The co-grinding mixture also exhibited a significant enhancement in the dissolution rate among the other mixtures. FTIR studies revealed the absence of drug-polymer interaction in the solid dispersions. Minor shifts in the endothermic peaks of the DSC thermograms of SD3 and CGM indicated slight changes in drug crystallinity. XRD studies further confirmed the results of DSC and FTIR. Topological changes were observed in SEM images of SD3 and CGM. In vivo pharmacodynamic studies indicated an improved efficacy of the optimized batch SD3 as compared to the pure drug at a dose of 3 mg/kg/day. Modified locust bean gum can be a promising carrier for solubility enhancement of poorly water-soluble drugs. The lower viscosity and wetting ability of MLBG, reduction in particle size, and decreased crystallinity of the drug are responsible for the dissolution enhancement of atorvastatin. The co-grinding mixture can be a good alternative to solid dispersions prepared by modified solvent evaporation due to its ease of preparation and significant improvement in dissolution characteristics. 相似文献