Evaluation of Class I ART restorations in Brazilian schoolchildren: three-year results

Atraumetic Restorative Treatment (ART) has been adopted around the world to avoid unnecessary extractions, especially in non-industrialized countries The development of specific glass ionomer cements marketed for the ART technique has contributed to the technical success rate. In this study. Ketac-Molar^a (3M ESPE. Dental Medzn Germany) was used to restore 150 Class I cavities in 118 Brazilian public school children, aged from 7–12 years. At baseline and at subsequent recalls. CPI probes with a ball-end of 0.5 millimeters (mm) were used to assess loss of restorative material, and photographic color transparencies of restorations were made. After six months. 83 patients returned for follow-up examinations, with 71.8% of their restorations designated as acceptable. After three years. 49 patients with 57 ART-restorations were evaluated, with 21.0% of these restorations graded as acceptable Another 29.8% of their restorations had been replaced by more permanent materials. The main objective of the ART technique is tooth retention; this was achieved for 94.7% of the restored teeth in a high caries risk population who returned for recalls.     

玻璃纤维表面的乙烯基单体接枝聚合

用玻璃纤维表面处理剂ＭＡＣ处理玻璃纤维后，再进行臭氧处理，使玻璃纤维的表面产生活性中心，引发苯乙烯、甲基丙烯酸甲酯、及丙烯酸等乙烯基单体在玻璃纤维表面上接枝聚合。接枝纤维的密度减小，对水的浸润性下降，红外光谱及扫描电镜观察证明玻璃纤维表面上接枝聚合物的存在。     

自酸蚀封闭剂与玻璃离子联合用于托槽粘结的临床观察

目的：评价自酸蚀粘结剂与树脂加强型玻璃离子联合用于正畸托槽粘结的临床使用效果。方法：随机选择50名患者，采用自身对照的方法进行为期12个月的临床观察。385个托槽用自酸蚀封闭剂（3MESEP）和光固化树脂加强型玻璃离子水门汀（FUJI ORTHO LC）粘结，390个托槽用京津釉质粘结剂粘结。观察托槽脱落的时间和脱落后粘结剂在牙面上的残留量，计算托槽脱落率和粘结材料残留指教。结果：光固化树脂加强型玻璃离子水门汀和自酸蚀封闭剂粘结的托槽脱落率为10．1％；京津釉质粘结剂粘结的托槽脱落率为9．0％，两者差异没有统计学意义（P〉0．05）。两种材料粘结的托槽脱落后，牙釉质上的粘结材料残留指教差异无显著统计学意义（P〉0．05）。结论：自酸蚀封闭剂和树脂加强型玻璃离子所构成的粘结技术粘结正畸托槽的粘结强度，可以满足正畸治疗的需要，在临床上有广阔的应用前景。     

东莨菪碱对大鼠体感区皮层神经元单位放电的影响

目的观察东莨菪碱对大鼠体感区皮层自发放电和诱发放电的影响。方法用玻璃微电极在大鼠体感区皮层记录神经元单位放电。结果实验记录了３５个单位放电，其中对触压后肢应答放电的有２２个，前肢９个；触前肢兴奋，后肢抑制的１个；触前肢抑制，后肢兴奋的１个；触毛及皮肤呈汇聚反应的２个。静注东莨菪碱后有２３个呈增频反应，１２个为减频反应。增频单位多分布在Ⅰ～Ⅳ层，减频单位则在Ⅴ～Ⅵ层。结论东莨菪碱对皮层体感区Ａｃｈ兴奋神经元或Ａｃｈ抑制神经元均有阻断作用。     

玻璃纤维诱导的转化细胞活化癌基因的研究

用玻璃纤维处理BALB/C3T3细胞,使细胞发生转化,从转化细胞中抽提DNA用以转染NIH3T3细胞,结果被转染的细胞亦发生转化。用~(32)P标记的Ha-ras癌基因作探针,通过斑点杂交,征明玻璃纤维诱发的BALB/3T3转化细胞中,Ha—ras的原癌基因被激活。     

Laser microdissection and pressure catapulting (LMPC) in paraffin sections mounted on glass slides. A methodological report

The technique of laser microdissection together with laser pressure catapulting (LMPC) is demonstrated in paraffin sections obtained from surgical specimens of brain tumors mounted on glass slides. A sufficient and precise application of microdissection techniques in tissue on glass slides is worthwhile, since it offers the possibility of a retrospective analysis of archived paraffin sections in histopathology. We could demonstrate a precise dissection of areas in tissues of different thicknesses (4 microm and 20 microm). Areas of tissue mounted directly on glass need to be dissected in a scanning mode in order to remove the total region in form of small tissue fragments row by row. This mode provided a precise microdissection of tissue areas of different sizes and shapes. A successful molecular biological analysis of the microdissected regions could be demonstrated. As an example for such an analysis, differential-PCR for detecting an amplification of the gene for the epidermal growth factor receptor (EGFR) was performed.     

玻璃离子水门汀氟离子的释放和再吸收及重量减少

观察缺氧及再给氧对体外培养星形胶质细胞存活能力及谷氨酸摄取功能的影响。方法：取生后１～２ｄ新生昆明小鼠大脑皮层进行星形胶质细胞原代培养,培养 １周左右予以缺氧。缺氧时间分别为 １２、２４、４８ ｈ,并取缺氧２４ ｈ后再给氧０、１２、２４、４８ ｈ的细胞观察其形态、死亡细胞数目、乳酸脱氢酶活性及谷氨酸摄取能力的变化。结果：缺氧组及再给氧组星形胶质细胞死亡数较对照组无明显变化,缺氧前、后乳酸脱氢酶活性亦无明显改变,缺氧组谷氨酸摄取功能较对照组下降３０％～６０％,并随缺氧时间延长而明显下降（与对照组相比,Ｐ＜０．０１）,再给氧２４ ｈ内谷氨酸摄取能力有所恢复,但未达正常水平。结论：星形胶质细胞与神经元相比,对缺氧培养液较为耐受,缺氧对星形胶质细胞存活能力无明显影响,但细胞摄取谷氨酸能力有所改变。     

Subtotal colectomy for obstructing carcinoma of the left colon

This retrospective review of seven patients with completely obstructing cancers of the left half of the colon, in addition to other reports in the literature, suggests that subtotal colectomy with primary ileal

1 Clinical data on seven patients who underwent subtotal colectomy for obstructing carcinoma of the left colon: 1975–1982.

Patient	Age (yr) and Sex	Tumor Location	Hospital Stay	Comments
1	68, F	Decending	10 days	A and W 40 mo postop1
2	71, F	Sigmoid	22 days	A and W 18 mo postop
3	73, F	Sigmoid	…	A and W 5 yr postop
4	66, F	Decending	8 mo	Dead from complications
5	72, M	Sigmoid	11 days	Incidental cecal cancer; A and W 3 mo postop
6	66, M	Sigmoid	28 days	Alive with metastasis 16 mo postop
7	78, M	Left transvers	34 days	Many other polyps; A and W 9 mo postop

1

A and W = alive and well.

proctostomy may be the treatment of choice for those lesions that are technically resectable and located high enough to permit an intraperitoneal ileal proctostomy. The morbidity and mortality is less than that seen with the staged approach and the length of hospitalization is shorter. By eliminating a second or third hospitalization and a temporary colostomy, palliation is better in those patients who ultimately die from recurrent cancer. Furthermore, those patients resected for cure may have increased rates of long-term survival.     

Photodynamic therapy in the bladder: Can the light dose be easily quantified?

The shape of the bladder and the optical characteristics of the tissue within the wall can be shown to play an important role in the amount of light actually received at the wall. The use of estimated doses even assuming spherical geometry, cannot therefore be relied upon. This paper describes some experimental work carried out on a glass model that was used to simulate the bladder. A specially constructed dual detector was used which consisted of an isotropic probe and semiconductor detector. This enabled measurements of irradiance and space irradiance (light energy fluence rate) to be made simultaneously. By changing the optical characteristics of the wall a four-fold increase in space irradiance was measured. Contamination of the water contained within the model by blood was also investigated and has shown that with concentrations as low as 0.5% the delivered light dose reaching the wall can be reduced by up to 50% at a wavelength of 510 nm. Some in vivo measurements are also presented together with some comments on the difficulties that have been encountered when transferring measurements from the model to the patient.