两种不同盖髓剂对人牙髓细胞毒性的体外研究

目的:研究丁香酚及Dycal对人牙髓细胞的毒性。方法:体外组织块法培养人牙髓细胞,采用噻唑蓝比色法(MTT法)及台盼蓝染色活细胞计数法来评价不同浓度及不同时间段丁香酚及Dycal对牙髓细胞细胞毒性,比色法测定丁香酚干预组细胞内总谷胱甘肽含量。结果:0.1mmol/L浓度的丁香酚及0.1%浓度的Dycal对人牙髓细胞毒性明显,其对细胞毒性反应成浓度及时间依赖性改变。各丁香酚组细胞内谷胱甘肽含量低于对照组,第3天丁香酚最低浓度组细胞内谷胱甘肽含量高于高浓度组。结论:高浓度丁香酚及Dycal对牙髓细胞毒性明显,细胞毒性与浓度及作用时间有关。低浓度丁香酚可能通过谷胱甘肽的参与抵抗氧化应激,从而起到对细胞的保护作用。Dycal可能更加适合用于间接盖髓。     

A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal

Background:  Permanent teeth pulp exposures have traditionally been treated with calcium hydroxide pulp capping. The aim of this study was to investigate the response of human pulp tissue which were mechanically exposed to a new material, Propolis and compare it with two existing and commonly used pulp capping agents (mineral trioxide aggregate and Dycal).
Methods:  Thirty-six intact human premolars were mechanically exposed. Teeth were divided into six groups of 6 teeth each and were capped with Propolis, mineral trioxide aggregate and Dycal. Final restoration was done with posterior composite resin using light cured glass ionomer cement as a liner. The teeth were then extracted on the 15th or the 45th day and processed for histological evaluation.
Results:  Differences in inflammatory response and dentine bridge formation of the exposed pulp to the three different materials were statistically calculated using chi-square test and were found to be non-significant. There was more pulp inflammation in teeth treated with Dycal than with Propolis and MTA on the 15th as well as on the 45th day. Propolis and MTA showed bridge formation in more teeth, and the bridges were in closer proximity to pulp capping material than teeth treated with Dycal on the 45th day.
Conclusions:  The response of pulps to Propolis as a pulp capping agent was comparable to MTA and Dycal.     

超声荡洗结合Dycal基质剂治疗年轻恒牙慢性根尖周炎

目的：观察超声荡洗和Dycal基质剂联合治疗年轻恒牙慢性根尖周炎的临床治疗效果。方法：在传统的根管治疗方法基础上加用根管超声荡洗再结合Dycal基质剂治疗20例患者的22颗年轻恒牙,其中,前牙10颗,双尖牙12颗。牙髓坏死原因分别为外伤8颗、龋病4颗、畸形中央尖折断10颗。结果：21颗治疗成功,1颗治疗失败,成功率为95.5%。结论：根管超声荡洗和Dycal基质剂联合用于年轻恒牙根尖周炎和根尖诱导成形术,是值得临床探索的治疗方法。     

Electron microscope characteristics of dentin repair after hydroxylapatite direct pulp capping in rats

In order to study the osteogenic action of hydroxylapatite (HA) on the dental pulp, a pulp capping experiment was designed using the rat upper molar. Under general anesthesia, molar teeth in 14 male Sprague-Dawley rats were pulp capped with Osteogen (HA) or with Dycal as a control material. After pulp capping, the maxillary molars cavities were restored with amalgam and a pedodontic steel crown was adjusted and sealed over the molar teeth on either side of the maxilla. After 7 days, the areas of necrosis and acute inflammation were more evident in the pulps treated with Dycal than with Osteogen. Hard tissue formation began to appear around dentinal chips in the pulp and extended from the cavity walls into the pulp regardless of the material that was used. Furthermore, this calcified material was scattered throughout the pulp when Osteogen was used, but was not observed in the Dycal treated pulps. The hard tissue formation was thought to be due to the putative fibroblasts and odontoblasts found in the pulp. After 28 days dense dentinal tissue was observed bridging the exposure site when Dycal was used. The dentinal tissues formed with Osteogen was always of a globular type, and showed an irregular distribution. Since Osteogen tends to cause areas of dystrophic calcification in the pulp, its use is not be recommended for pulp capping purposes in humans, because these areas of calcification would make future endodontic treatment difficult.     

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目的观察Dycal和矿物三氧化物凝聚体（MTA）在大鼠磨牙直接盖髓后骨形态发生蛋白（BMP）-2的表达变化。方法本研究于2011年3月至2012年12月在中国医科大学口腔医学院中心实验室及辽宁省口腔医学研究所进行。42只雌性Wistar大鼠双侧上下第一磨牙分别用Dycal和MTA直接盖髓，在各观察时点（盖髓后12h及1、3、7、14、21、28d）处死动物取材，进行BMP-2免疫组织化学染色，用图像分析软件测定各组标本中BMP-2阳性染色的平均光密度值。用SPSS13．0统计软件对数据分别进行配对t检验和Dunnett-t检验。结果在正常牙髓组织中BMP-2表达呈阴性，Dycal和MTA直接盖髓后12h牙髓中BMP-2出现低水平表达并逐渐增强，7d时达到高峰，14d后表达开始下降，至28d时接近正常水平。MTA组修复性牙本质形成较Dycal组连续且致密，7、14d时MTA组BMP-2表达强度高于Dycal组。结论MTA组形成修复性牙本质较Dycal组连续且致密，BMP-2的表达高于Dycal组；MTA可能通过调节BMP-2的表达参与诱导修复性牙本质的形成，BMP-2的表达强度可能影响了牙髓损伤修复。     

不同剂型氢氧化钙对深龋与深龋意外盖髓的临床效果比较

目的　评价传统氢氧化钙和Dycal分别用于间接和直接盖髓的临床效果。方法　选择深龋和深龋意外穿髓的患者 180例共 2 2 5颗患牙分成 2组 ,分别使用Dycal和传统氢氧化钙进行间接或直接盖髓治疗。结果　 2者用于深龋间接盖髓在疗效上无显著性差异 ,Dycal应用于深龋意外穿髓盖髓治疗效果较好 ,成功率高于传统氢氧化钙盖髓。结论　Dycal是目前比较理想的盖髓材料     

Dycal和粉剂氢氧化钙直接盖髓疗效的临床观察

目的 观察Dycal和粉剂氢氧化钙直接盖髓的临床疗效并比较.方法 将要做直接盖髓的患牙,随机分成A、B两组,分别进行Dycal和粉剂氢氧化钙直接盖髓术,半年后复查并比较.结果 A组成功率为90.8%,B组成功率为77.1%,两组有显著差异(P＜0.05).结论 直接盖髓Dycal的疗效优于粉刺氢氧化钙.     

Dycal直接盖髓在畸形中央尖折断治疗中的应用

选择2006-07～2008-09济南市口腔医院儿童牙科就诊的畸形中央尖患者36例,患牙58颗。分别采用调磨、Dycal直接盖髓、活髓切断术治疗畸形中央尖患牙,追踪观察2年,以了解不同治疗方法在预防畸形中央尖折断的临床疗效。总结分析以后,我们认为是早期局麻下去除中央尖用Dycal直接盖髓的方法更为可取。     

医用生物胶加氢氧化钙盖髓的实验研究

目的：观察ＺＴ医用胶加氢氧化钙作为盖髓剂的性能。方法：分别用ＺＴ医用胶加氢氧化钙、ＺＴ医用胶、氢氧化钙和Ｄｙｃａｌ进行狗牙盖髓实验，２只狗共４０个牙，观察４、８周。结果：４、８周时４种材料的牙髓组织学反应基本相似：牙髓组织反应较轻，无坏死层出现，除４周时Ｄｙｃａｌ组有２例出现微小脓肿外无明显炎细胞浸润，８周时ＺＴ医用胶加氢氧化钙组和Ｄｙｃａｌ组均有钙化组织出现。结论：上述结果说明ＺＴ医用胶加氢氧化钙的盖髓性能不亚于Ｄｙｃａｌ。