下颌磨牙区即拔即种的临床疗效观察

目的 探究下颌磨牙区即拔即种的临床疗效。方法 选取2017年1月至2019年1月江门市口腔医院收治的60例需进行下颌磨牙区种植的患者随机分为A、B组,A组给予即拔即种,B组给予延期种植,比较两组留存率、浅蝶形骨吸收率、种植12个月后PIS指标及满意度进行评价。结果 随访12个月两组留存率均为100%,浅碟形骨吸收率A组前磨牙为16.67%、磨牙为22.22%,B组前磨牙为10.00%、磨牙为25.00%,差异均无统计学意义(P＞0.05),A组植入30个种植体,60个牙间乳头,其中3度29个、2度24个、1度7个,B组植入30个种植体,60个牙间乳头,其中3度14个、2度24个、1度22个,A组牙间乳头PIS指标优于B组,且A组牙间乳头PIS评分(2.36±0.52)较B组(1.87±0.48)显著提高,差异均具有统计学意义(P＜0.05),A组对治疗效果满意率为93.33%较B组66.67%显著提高,差异具有统计学意义(P＜0.05)。结论 对于下颌磨牙区缺牙患者可采取即拔即种的方法修复,缩短缺牙时间,促进咬合和咀嚼功能恢复,舒适且美观,提高患者的满意度,值得在临床上推广。     

Conjunctival flap advancement with or without scleral graft for hypotony maculopathy after trabeculectomy

AIM: To introduce a novel technique for transscleral fixation of the PC-IOL that requires no sutures on the IOL haptics. METHODS: Instead of suturing polypropylene onto the IOL haptics, the method simply winds the thread on the haptics. Fifteen eyes of 15 patients underwent this technique and were followed up for more than 18 months. Surgical outcomes and post-operative complications were evaluated and compared with those of the conventional transscleral fixation method. RESULTS: Postoperative cylinder was significantly lower in the thread winding group than in the conventional transscleral fixation method group (-1.02±0.46 diopters vs -1.57±0.77 diopters; P=0.01). Further, no postoperative complications, such as optic capture, IOL dislocation, and hyphema, were detected in the thread winding group. CONCLUSION: We believe that our thread winding technique is better than previously reported methods because it is simple, mechanically stable, and free from suture-related complications.     

影像学评估在耳蜗植入术中的价值

目的：评价影像学检查在耳蜗植入术中的价值。方法：37例感音性神经性聋患者术前常规行颞骨HRCT及MRI检查，术后行改良斯氏位X线拍片，其中5例术后行CT复查。结果：CT显示2例Mondini畸形（4耳），2例前庭导水管扩张（4耳），1例迷路骨化（1耳），1例内听道狭窄（2耳）。MRI显示Mondini畸形3例（6耳），前庭导水管扩张2例（4耳），1例并内淋巴囊扩张（2耳），2例迷路纤维化（2耳）。术后改良斯氏位X线拍片显示植入电极形态、位置良好。CT显示1例电极滑脱。结论：CT及MRI对耳蜗植入术术前评估具有重要价值，改良斯氏位X线拍片结合CT对术后评估更有意义。     

A clinical and radiological two‐year follow‐up study of maxillary overdentures on osseointegrated implants

In order to satisfy the need to restore the aesthetics, phonetics and comfort and to facilitate optimal hygiene procedures, 20 edentulous patients were treated with a new concept of overdenture therapy on implants ad modum Brånemark. After 24±3.5 months the patients were re‐examined. They were asked to answer a questionnaire and use a Visual Analogue Scale (VAS) to give their opinion on the prosthetic treatment. The results indicate that an implant‐retained overdenture in the maxilla with this design can satisfy the patients needs in aesthetics, phonetics and comfort and can 1 facilitate oral hygiene measures.     

Screening Mouse Genes Related to Blastocyst Implantation by Using PCR Subtraction

Objective To identify genes that may be related to embryo implantation Materials & Methods The PCR subtraction technique was applied at implantation and inter-plantation sites on day 4. 5 of pregnancy in mice. Two novel Expressed Sequence Tags (ESTs ), EST8 and EST81 were identified; their expression in tissues was analyzed by Northern blotting, and their full-length cDNAs were synthesized by PCR.Results We found that these two novel ESTs (EST8and EST81) were noticeably expressed in implantation site in the mouse on day 4. 5 of pregnancy. EST8 was expressed at high level in livers and implantation sites of the mice, while at low level in ovaries and inter-plantation sites. EST81 was predominantly expressed in implantation site and ovary, and at low level in all other tissues. Their complete cDNAs, 1 665bp and 1 264 bp respectively, were synthesized by using PCR.Conclusion The two full-length cDNAs were responsible for embryo implantation,and their functions need to be further studied.     

Entrapment of pacemaker lead by a large netlike Eustachian valve within the right atrium

Summary During pacemaker implantation in a patient with permanent atrial fibrillation, it remained impossible to advance a passive fixation lead with fins through the right atrium. However, a lead with a retractable screw easily passed the right atrium and was positioned in the right ventricle. Transesophageal echocardiography revealed an extensive net–like perforated Eustachian valve within the right atrium that had caused entrapment of the anchor fins during lead implantation. Remnants of embryonal structures within the right atrium should be considered a rare possible barrier during pacemaker implantation.     

生物瓣膜失功能原因分析

目的对植入人体内1年以上损坏的生物瓣膜作损坏原因的临床分析。方法1993年6月至2004年6月,共41例(47只)生物瓣膜失功能病人行再次手术换瓣。两次换瓣间隔时间1~16年,平均(9.7±4.1)年。对失功能生物瓣膜进行标本观察及病理学检测。结果47只带支架生物瓣膜中,瓣架无一损坏,42只瓣膜发生瓣叶撕裂或穿孔,35只有不同程度的钙化现象,且在瓣环表面和瓣脚周围有纤维组织增生,2只瓣膜有赘生物及血栓形成;47只生物瓣膜中损坏较重的瓣膜送病理学检查发现大量吞噬细胞和单核细胞浸润。结论生物瓣膜早期损坏以破损撕裂为主,晚期以钙化和破损撕裂等多种因素的复合病损结果出现。     

血管内超声显像在冠心病支架植入术中的应用

目的探讨血管内超声在冠心病支架植入中的作用。方法50例患者的52处病变在支架植入前后分别用血管内超声进行定量和定性分析,并根据血管内超声标准决定支架的直径以及植入的终点,分析CAG和IVUS对支架植入终点判断的差异和最终获得的管腔面积大小的差别以及支架后管腔面积增大的机制。结果IVUS比CAG判断的平均支架直径大[(3.48±0.29)mmvs(3.36±0.33)mm,P=0.011],支架囊的最终峰值压力明显增大[(17.7±2.9)atmvs(12.8±2.4)atm,P<0.001],QCA测得的支架面积狭窄百分比减小(13.2%±6.6%vs16.6%±9.1%,P=0.044);首次高压扩张后支架满意率CAG达96.2%,而IVUS只有37.7%。IVUS指导后最终的球囊压力更高[(16.13±1.87)atmvs(12.62±2.61)atm,P<0.001],获得的管腔直径更大[(3.64±0.53)mmvs(3.31±0.57)mm,P<0.001],管腔面积也更大[(9.90±2.05)mm2vs(8.84±1.67)mm2,P<0.001],面积狭窄百分比更小(49.15%±9.03%vs54.24%±10.05%,P<0.001];所有患者支架的近段和远段CAG均未发现明显的狭窄。而IVUS却发现支架近段血管有39例(75.0%),远段血管有23(44.2%)例存在动脉粥样硬化斑块;支架植入后非脂质斑块较脂质斑块获得的管腔面积更大[(4.50±1.67)mm2vs(3.68±0.97)mm2,P<0.001],其中脂质斑块血管面积增大较非脂质斑块小1.30mm2,斑块压缩程度却增加0.48mm2。结论IVUS较CAG能更好地判断病变的性质,指导支架更好地选择,可获得更大的管腔面积,更小的面积狭窄百分比。     

17β-雌二醇对子宫内膜异位症患者在位子宫内膜间质细胞β-catenin mRNA和蛋白表达的影响

目的研究17β-雌二醇(17β-E2)对子宫内膜异位症(内异症)患者在位子宫内膜间质细胞β-catenin mRNA和蛋白表达的影响,探讨Wnt/β-catenin信号通路在介导雌激素促进内异症发生发展的作用。方法体外分离培养内异症患者在位子宫内膜间质细胞。用不同浓度17β-E2处理子宫内膜间质细胞48 h;此后选用10-10mol/L 17β-E2处理子宫内膜间质细胞12、24和48 h,逆转录聚合酶链反应(RT-PCR)和免疫印迹法(Western blotting)检测17β-E2处理前后子宫内膜间质细胞β-catenin mRNA和蛋白的表达水平。同法分析雌激素受体拮抗剂ICI182,780(10-6mol/L)对17β-E2促进β-catenin mRNA和蛋白表达的影响。免疫组织化学染色观察17β-E2作用后β-catenin在子宫内膜间质细胞中的定位。结果17β-E2能明显促进内异症患者在位子宫内膜间质细胞β-catenin mRNA和蛋白的表达,并呈剂量和时间依赖性,于10-10mol/L作用48 h最明显。雌激素受体拮抗剂ICI182,780能明显抑制17β-E2对子宫内膜间质细胞β-catenin mRNA和蛋白的表达。免疫组织化学染色发现17β-E2能促进β-catenin在子宫内膜间质细胞核内的表达。结论雌激素可能通过激活Wnt/β-catenin信号通路促进内异症在位子宫内膜的异位种植。