大孔树脂分离纯化山楂总黄酮的研究

目的研究大孔吸附树脂分离纯化山楂总黄酮的工艺条件。方法以紫外可见分光光度法测定的山楂样品溶液中总黄酮的含量为指标,采用正交试验和单因素考察相结合的方法考察多个工艺参数。结果D101型大孔树脂对山楂的总黄酮有良好的吸附,其吸附分离工艺条件的药液浓度为0.1g.ml-1、pH3~4,上样量为4倍柱体积,以1m l.min-1吸附速率进行吸附,4.5倍柱体积的80%乙醇洗脱效果最佳。结论方法简单易行,分离效果良好,适于山楂中总黄酮的分离纯化。     

磨牙残根残冠分根保存修复的临床应用

目的 尽可能保存磨牙的残根残冠,提高患牙的保存率.方法 选择磨牙的残根残冠,经完善的根管治疗后,采用分根法作保存修复.结果 经1年观察,保存成功率91.2%,患牙能正常行使咀嚼功能.结论 应用分根后修复是提高磨牙残冠保存率的好方法.     

经阴道水压分离全子宫切除99例分析

目的探讨经阴道水压分离全子宫切除术的手术方法。方法术中在子宫颈周围间隙先行用含有1∶1000肾上腺素的生理盐水注入黏膜下水压分离。结果本组99例经本术式治疗均获满意效果。术中出血少,术野干净,解剖层次清晰,不易损伤膀胱、直肠及周围组织。结论经阴道水压分离全子宫切除术是一种安全、有效、损伤小、费时少、恢复快的术式,符合微创原则的手术方法。     

松凝分筋手法为主结合玻璃酸钠注射治疗膝关节骨关节炎的临床研究

目的:通过中西医结合的临床对比观察研究,进一步证实松凝分筋手法治疗膝关节骨关节炎的临床疗效。方法:选择96例膝关节骨关节炎患者,随机分为治疗组和对照组。治疗组以松凝分筋手法治疗为主,对照组单纯用玻璃酸钠行膝关节腔注射,疗程5周,并观察疼痛、功能、活动度、肌力、屈曲畸形及稳定性等指标。结果:治疗组和对照组治疗后各项指标均有明显的改善,治疗前后差异明显(p<0.01),其中治疗组对于疼痛、功能、活动度、屈曲畸形的改善明显优于对照组(p<0.05)。结论:松凝分筋手法具有良好的缓解疼痛、改善膝关节功能的作用。     

新公共管理视野下卫生领域政事分开的理论与实践探讨

新公共管理理论为中国卫生领域政事分开奠定了理论基础。按照其掌舵而非划桨的思想,卫生领域改革应该正确处理好政府和事业单位的关系。文章简述了卫生领域政事分开不彻底和政府过度放权而监督不力两种相反方向的政府失灵现状,提出卫生领域政事分开改革需要处理好的医院管理法人选择和政府职能定位两个关键问题。     

消风散主要药效学及拆方研究

消风散具有明显的止痒和抗实验性荨麻疹作用。拆方研究证实,疏风散为君药且起主导作用,再加祛湿药为最基本的治疗原则。     

脐血CD34+细胞的分离与纯化

脐血造血干细胞的分离是进行干细胞移植、体外扩增培养的关键.研究采用明胶自然沉降法、Fi-coll分层法分离脐血MNC后,用MACS分离纯化CD34+细胞,计数并用流式细胞仪进行纯度分析.结果显示每份脐血的采集量平均为95±52 ml,3 g/dl明胶自然沉降法所获MNC密度平均为(5.76±0.67)×107/ml;CD34+细胞密度平均为(5.53±1.16)×105/ml,较Ficoll分层法高(P＜0.01).因此,3 g/dl明胶自然沉降法是一种较理想的分离MNC的方法,用明胶沉降法和MACS相结合是分离脐血CD34+细胞的理想方法.     

淋巴细胞分层液在人绒毛膜滋养层细胞分离纯化中的应用

目的建立一种简便易行、经济有效的纯化培养滋养层细胞的方法。方法取妊娠6～8周的正常绒毛组织10例,采用胰蛋白酶消化法分离细胞,将细胞随机分为两组(各5例),一组用淋巴细胞分层液进行纯化,另一组未纯化,最后用含15%的胎牛血清的培养基培养细胞。用免疫细胞化学法进行细胞纯度鉴定。结果未纯化的细胞阳性率为(68.62±2.69)%,纯化后的细胞阳性率为(91.60±1.55)%,两者之间的差异具有极显著性意义(P<0.01)。结论应用淋巴细胞分层液可获得纯度较高、合乎试验要求的细胞滋养层细胞。     

Improving condition severity classification with an efficient active learning based framework

Classification of condition severity can be useful for discriminating among sets of conditions or phenotypes, for example when prioritizing patient care or for other healthcare purposes. Electronic Health Records (EHRs) represent a rich source of labeled information that can be harnessed for severity classification. The labeling of EHRs is expensive and in many cases requires employing professionals with high level of expertise. In this study, we demonstrate the use of Active Learning (AL) techniques to decrease expert labeling efforts. We employ three AL methods and demonstrate their ability to reduce labeling efforts while effectively discriminating condition severity. We incorporate three AL methods into a new framework based on the original CAESAR (Classification Approach for Extracting Severity Automatically from Electronic Health Records) framework to create the Active Learning Enhancement framework (CAESAR-ALE). We applied CAESAR-ALE to a dataset containing 516 conditions of varying severity levels that were manually labeled by seven experts. Our dataset, called the “CAESAR dataset,” was created from the medical records of 1.9 million patients treated at Columbia University Medical Center (CUMC). All three AL methods decreased labelers’ efforts compared to the learning methods applied by the original CAESER framework in which the classifier was trained on the entire set of conditions; depending on the AL strategy used in the current study, the reduction ranged from 48% to 64% that can result in significant savings, both in time and money. As for the PPV (precision) measure, CAESAR-ALE achieved more than 13% absolute improvement in the predictive capabilities of the framework when classifying conditions as severe. These results demonstrate the potential of AL methods to decrease the labeling efforts of medical experts, while increasing accuracy given the same (or even a smaller) number of acquired conditions. We also demonstrated that the methods included in the CAESAR-ALE framework (Exploitation and Combination_XA) are more robust to the use of human labelers with different levels of professional expertise.     

分离胶采血管对部分临床生化检验测定结果的影响

目的观察用肝素锂分离胶真空采血管分离血浆对部分临床生化检验项目测定结果的影响。方法采集同一供血者的空腹肘前正中静脉血,一针三管,分别置于无任何添加剂的干燥真空采血管、肝素钠抗凝的真空采血管和肝素锂分离胶真空采血管中,分离血清和血浆,2h内在全自动生化分析仪上测定部分临床生化检验项目,对3纽测定结果进行分析比较。结果白蛋白（ALB）测定值,肝素锂分离胶管低于血清管（P=0．016〈0．05）;血糖（GLU）测定值,肝素锂分离胶管高于血清管（P=0．020〈0．05）,其余指标3组间差异均无统计学意义（P〉0．05）。结论肝素锂分离胶真空采血管分离血浆可能会使ALB的测定值略偏低,而使GLU的测定值略偏高,但对TP、T．BIL、D—BIL、GGT、UA、URE．A、HDL、LDL、CH0无明显影响。