Cytokine regulation of human monocyte interleukin-1 (IL-1) production in vitro. Enhancement of IL-1 production by interferon (IFN) gamma, tumour necrosis factor-alpha, IL-2 and IL-1, and inhibition by IFN-alpha.

IL-1 production (secreted and cell-associated) was measured in monocyte cultures stimulated by a variety of agents in vitro. Monocytes either adherent to conventional plastic culture plates in serum-free conditions, or in suspension in culture medium containing serum were stimulated to produce IL-1 during culture. In non-adherent, serum-free conditions, monocytes produced very low or undetectable amounts of IL-1 during 20 h of culture. Lipopolysaccharide (LPS) induced equivalent amounts of secreted and cell-associated IL-1, although at very low concentrations more cell-associated IL-1 was produced. IL-1 production in response to LPS could be augmented by crude lymphokine, IFN-gamma, or tumour necrosis factor (TNF) alpha. TNF-alpha preferentially augmented the production of cell-associated IL-1 in LPS-stimulated cultures. TNF-alpha induced a significant amount of IL-1 (mainly cell-associated) directly but could also induce IL-1 secretion when combined with IL-2 or IFN-gamma, or when in the presence of serum. IL-2 acted synergistically with low concentrations of IFN-gamma or IL-1 to induce significant levels of IL-1 production. IFN-alpha did not induce any IL-1 production, but was a potent inhibitor of IL-1 production induced by a variety of stimuli. These results suggest that IL-1 production may be enhanced or reduced by different cytokines at concentrations likely to be found in chronic inflammatory lesions.     

细菌溶解产物胶囊对小儿哮喘及血清IL4、IFN-y、IgE的影响

目的：探讨细菌溶解产物胶囊（泛福舒）对小儿哮喘疗效以及对血清中白细胞介素-4（IL-4）、γ-干扰素（IFNγ）以及免疫球蛋白E（IgE）的影响.方法：将64例急性发作期支气管哮喘患儿随机分为观察组（n=31）和对照组（n=33）,对照组予支气管扩张剂及吸入糖皮质激素等常规治疗,观察组在此基础上加用泛福舒治疗,观察两组患儿治疗前后肺功能及血清IL-4、IFN-γ以及IgE的变化.结果：治疗3个月后两组患儿第1秒时间肺活量（FEVl）、呼气峰流速（PEF）水平均有显著提高,但观察组上述指标明显优于对照组,差异均有统计学意义（P＜0.05）;对照组血清IL-4、IFN-γ及IgE水平较治疗前后无明显变化（P＞0.05）,但观察组治疗后IL-4、IgE明显下降,IFN-γ明显提高,较治疗前差异有统计学意义（P＜0.05）.结论：泛福舒能够改善哮喘患儿的肺功能,降低血清IL-4、IgE水平,升高IFN-γ水平,纠正辅助性T细胞1辅助性T细胞2（Th1/Th2）的偏移,调整患儿的免疫异常.