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排序方式: 共有98条查询结果,搜索用时 15 毫秒
1.
B细胞杂交瘤技术制备抗同种特异T细胞膜抗原单克隆抗体 总被引:1,自引:0,他引:1
目的:为进一步分析TCV免疫诱导同种免疫反应低下的机制。方法:采用B细胞杂交瘤技术获得分泌单克隆抗体的杂交瘤细胞。结果:两次的细胞融合中共得到12株稳定分泌单抗的杂交瘤细胞,为分析抗体在TCV中的作用提供条件。结论:TCV免疫可引起抗TCV细胞抗体的产生,以同系免疫的方法得到的活化B细胞用于B细胞杂交生产单抗是可行的。 相似文献
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Tarner IH Nakajima A Seroogy CM Ermann J Levicnik A Contag CH Fathman CG 《Clinical immunology (Orlando, Fla.)》2002,105(3):304-314
Rheumatoid arthritis (RA) is an autoimmune arthritis, for which treatment options remain limited. This study investigated the potential role of adoptive cellular gene therapy as a novel means for treating the RA animal model collagen-induced arthritis (CIA). Adoptive transfer of antigen-specific T-cell hybridomas retrovirally transduced to express IL-4 1 day before booster immunization significantly reduced the number of inflamed joints. Cell transfer after clinical onset of disease had no therapeutic effect. Bioluminescence imaging showed that the hybridomas migrated to the inflamed joints, thus delivering the regulatory protein locally at the site of inflammation. The homing was, at least in part, due to chemotaxis in response to proinflammatory chemokines that are expressed in inflamed joints. There were no significant changes in the cytokine milieu of the draining lymph nodes, nor in the systemic levels of anti-collagen antibodies in treated mice. We conclude that the beneficial clinical effects observed in our model were most likely based on the local action(s) of IL-4 in the inflamed joints and that the local delivery (and effects) of regulatory cytokines, like IL-4, constitutes a novel and effective method of preventing organ-specific autoimmune disease and of minimizing systemic adverse effects. 相似文献
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A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) has been developed using T cell hybridomas as coating antigen, for detection of Fc receptors for IgA (Fc alpha R). T-T hybridomas were generated from fusions of Fc alpha R+ T cell clones from mouse Peyer's patches with the Fc alpha R- R1.1 T lymphoma cell line. The 2 T-T hybridomas (designated Th HA) used here express Fc alpha R as determined by a rosette method and by ELISA. Th HA cells were cultured under conditions for maximum Fc alpha R expression, were added to individual wells of 96-well EIA plates, and were fixed in situ with glutaraldehyde. Plates were incubated with purified mouse monoclonal IgA, IgM or IgG1 and were developed with beta-galactosidase-coupled goat IgG antibodies specific for mouse heavy chains. Using the ELISA, both Th HA cell lines were shown to express significant levels of Fc alpha R, lower but detectable Fc mu R, and no discernible Fc gamma 1R. Interestingly, the rosette assay only allowed detection of receptors for IgA. When splenic lymphocytes were used, good Fc mu R and less Fc alpha R expression occurred on these cells as determined by ELISA and rosetting; however, no Fc gamma 1R cells were detected by either method. Thus, the ELISA is sensitive and reproducible, and allows an objective measurement of FcR expressed on T cells. 相似文献
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Trinh T. Tran 《Autoimmunity》2013,46(4):301-304
NZB (H-2d) mice are well known for the production of IgM autoantibodies to ssDNA. However, an FI cross between NZB and either NZW or SWR mice is required to produce IgG nephritogenic antibodies to dsDNA and glomerulonephritis. The contribution of parental class II loci in the hybrid mice is clearly important to the development of anti-dsDNA antibodies, In contrast, NZB mice congenic with the labm12 mutation develop IgG autoantibodies to dsDNA despite being homozygous for Ia. As a part of our effort to examine the mechanisms of disease development in NZB.H-2bm12 mice, we have generated a panel of monoclonal antibodies against nucleic acids. A subgroup of these antibodies exhibited strong electrostatic interaction with nucleic acids as evidenced by inhibition of their binding by a moderate increase in ionic strength. Interestingly, the effect of salt was either all or none; e.g., antibodies were either markedly inhibited or virtually unaffected. The importance of this ionic interaction was highlighted by analysis of DNA binding of antibodies from serum and nephritic kidneys of NZB.H-2bm12 mice. Antibodies specific for ssDNA, which are common in NZB mice and not associated with nephritic lupus, are largely unaffected by salt. However, serum and kidney eluted IgG antibodies specific for dsDNA were markedly inhibited by salt. We postulate that B cell clones whose antibodies exhibit electrostatic interaction with DNA are preferentially expanded during the course of lupus in NZB.H-2bm12 mice and that such antibodies contribute significantly to glomerulonephritis. 相似文献
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利用人工合成的白细胞间素2(IL-2)多肽片段,用中和试验、竞争ELISA和染色IL-2分泌细胞的碱性磷酸酶抗碱性磷酸酶桥联酶标技术,对己制备的抗人IL-2多克隆抗体及单克隆抗体(McAb)的表位特异性、中和活性、亲和常数及杭天然IL-2的反应性进行了全面鉴定。结果表明:利用多种免疫方案、融合前强化加强免疫及多个脾脏混合融合制备策略所获得的McAb,在生物学特征上表现出多样化,即这些McAb的表位特异性、中和活性、亲和力及抗天然IL-2活性各不相同。其中抗IL-2 C末端的McAb为首次报道。这组McAb适用于对IL-2研究的不同目的。 相似文献
8.
抗伏马菌素B1单克隆抗体的制备和特性 总被引:2,自引:0,他引:2
目的建立敏感、特异和快速的针对伏马菌素B1(fumonisin B1, FB1)的酶联免疫吸附试验(enzyme-linked immunosorbent assay ,ELISA)检测方法,形成具有我国自主知识产权的快速检测试剂盒.方法利用B细胞杂交瘤技术,建立能分泌抗伏马菌素B1单克隆抗体的杂交瘤细胞株.结果用伏马菌素B1-牛血清白蛋白(FB1-BSA)偶联物免疫8~10周龄雌性BALB/c小鼠后,取脾细胞与小鼠骨髓瘤细胞系Sp2/0融合,经过3~4次亚克隆建立了1个稳定分泌抗伏马菌素B1毒素抗体的杂交瘤细胞株,命名为5A9.将杂交瘤细胞打入BALB/c小鼠腹腔,获得含抗伏马菌素B1毒素单克隆抗体的腹水,并将腹水用饱和硫酸铵法进行纯化,得到单克隆抗体.该单克隆抗体的Ig亚类为IgG1,腹水中抗体的滴度分别为2.56×106,参考工作浓度为3.2×105.纯化后抗体的IgG含量为10.4 g/L,亲和常数为8.3×10-8 mol/L.该抗体与其他结构类似物无交叉反应,具有较高的特异性.结论制备了具有高特异性和亲和力的抗伏马菌素B1单克隆抗体,初步形成了针对伏马菌素B1的ELISA检测试剂盒. 相似文献
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