透明质酸钠对大鼠成肌细胞增殖和分化的影响

目的 研究透明质酸钠对成肌细胞增殖和分化的影响。方法 采用酶消化法将新生SD大鼠骨骼肌组织分离、纯化、原代培养及传代培养；取第3代成肌细胞，分别加入浓度为0．05%、0．1％及0．2％的透明质酸钠溶液作为生长培养基行体外培养为实验A、B及C组，加入常规生长培养基为对照D组，观察各组成肌细胞增殖情况，并采用细胞计数及MTT法绘制生长曲线。另选择0．1%透明质酸钠融合培养基行体外成肌细胞培养，常规融合培养基作对照，观察透明质酸钠对成肌细胞分化功能的影响。结果A、B及C组成肌细胞增殖表现相似，2d时进入对数生长期，4d时均达顶峰；D组成肌细胞于3d时进入对数生长期，5d时细胞数倍增，6d时达顶峰。MTT法所测吸光度（A）值的变化反映成肌细胞的增殖情况，与细胞计数的结果一致，以B组细胞增殖作用最明显，B组A值在2～8d时均高于C、D组（P〈0．05），8d时高于A组（P〈0．05）。0．1％透明质酸钠融合培养基中的成肌细胞融合率较低且上升缓慢，7d时融合率最高，为11．7％；常规融合培养基成肌细胞融合率于6d时达到峰值，约为35．0%。结论 透明质酸钠与成肌细胞的细胞相容性较好，可以作为良好的成肌细胞培养基。     

透明质酸钠玻璃体腔内充填在严重开放性眼球损伤治疗中的应用

目的 研究透明质酸钠玻璃体腔内充填对严重开放性眼球损伤预后的影响。方法 在严重开放性眼球损伤一期手术缝合后玻璃体腔内充填透明质酸钠至眼压Tn。结果 用透明质酸钠在玻璃体腔内充填，眼球萎缩的发生率（6．67％）明显低于用BSS液充填的发生率（70．58％）。结论 透明质酸钠玻璃体腔内充填在严重开放性眼球损伤治疗中的应用对眼球外形维持和视功能恢复有重要作用。     

Local injection of a mixture of beta-aminopropionitril fumarate and sodium hyaluronate together with controlled exercise for treatment of subacute superficial digital flexor tendonitis in horses

Local injection of a mixture of beta-aminopropionitril fumarate (BAPN-f) and sodium hyaluronate (NaH⁺) together with controlled exercise were evaluated for treatment of superficial digital flexor tendon (SDFT) injuries in horses. Fourteen mixed breed horses with subacute SDF tendon injuries in forelimbs were randomly assigned to two groups. One group received BAPN-f (0.7 mg/ml) and the other received NaH⁺ (10 mg/ml) all by intratendinous injection. Controlled exercise started during the first week after intratendinous treatment and continued for 12 weeks. Cross-sectional area (CSA) of the SDFT, diameter of the SDFT, and relative area of the lesion (presence of CSA) were measured by ultrasonographic examination. Lesions were semiquantitatively graded for echogenicity on a scale of 0 to 4. The lesion severity in CSA was significantly reduced by BAPN and NaH compared to those horses treated by BAPN only (p < 0.05). Lesion echogenicity score was significantly higher in horses treated with BAPN and NaH⁺ at day 0 compared to horses treated with BAPN (p < 0.05). At the end of the study, lesion echogenicity score was significantly reduced in the horses treated with BAPN and NaH⁺ compared to horses treated by BAPN only (p < 0.05). According to the results of the present study, a combination of BAPN-f and NaH⁺ has a greater beneficial effect on tendon healing and remodeling in horses, as assessed by sonographic examination, compared to treatment with single drugs.     

Structural-mechanical properties and swelling of protein complexes of hyaluronate and protein-chrondroitin-4-sulfate

Structural-mechanical strength and swelling of gels consisting of gelatin and potassium hyaluronate (PHY) or potassium protine-chondroitin-4-sulfate (PPCS) were shown to depend on the ratio between the components. With low concentrations of PHY and PPCS the minimum of structural-mechanical strength coincided with the maximum of swelling of the gels. In zones of neutralization of the positive electric charges of gelatin by macropolyanions, high structural-mechanical strength of the gels coincided with the minimum of swelling. In high concentrations of PHY, structural-mechanical strength and swelling of the gel became equal to the values characteristic of a gel consisting of gelatin only, but in high concentration of PPCS there was an additional parallel increase in this strength and in swelling of the gel.(Presented by Academician of the Academy of Medical Sciences of the USSR S. S. Debov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 10, pp. 1211–1213, October, 1976.     

Cell adhesion receptor expression during melanoma progression and metastasis

Summary Many steps in melanoma metastasis involve cell-cell or cell-matrix adhesive interactions. The surface molecules which mediate these processes therefore play an important role in regulating melanoma dissemination and their level of expression may alter during the course of tumor progression. Human melanocyte strains and melanoma cell lines have been characterised with regard to levels of cell surface receptors of the integrin family. Increased amounts of at least two integrins, VLA-4 (₄₁) and VnR (_V3), appeared to correlate with progression in this tumor, type. A novel VnR composed of an _V1 association has been observed in one melanoma cell line and there is the possibility that heterogeneity of integrin composition could affect biological behavior of these tumors.CD44, a cell surface glycoprotein which functions as the major receptor for hyaluronate, is another molecule whose expression increases in transformed cells of the melanocytic lineage. Iterative sorting on the FACS for stable variants, of both human and murine melanomas, expressing low and high levels of CD44 established that lack of expression of this molecule correlated with impaired ability to form pulmonary tumor nodules subsequent to i.v. injection into appropriate recipient mice. These findings illustrate that an understanding of the regulation of melanoma adhesion receptors could provide insights into the process of tumor spread.     

玻璃酸钠与Kenacort-A联合治疗膝骨关节炎合并滑膜炎临床观察

目的探讨玻璃酸钠(SH)联合肾上腺皮质激素类药Kenacort-A关节腔内注射治疗膝骨关节炎(OA)合并滑膜炎(SY)的临床疗效。方法选膝OA合并SY患者205例,随机分为单纯用药组(100例)和联合用药组(105例),单纯用药组给予SH关节腔内注射,联合用药组给予AH+Kenacor-A关节腔内注射。结果联合用药组的起效时间及疗效明显优于单纯用药组。结论SH联合Kenacort-A治疗膝OA合并SY,疗效快,可提高临床治疗效果。     

视网膜脱离复位动物模型的建立

目的探索在兔眼建立视网膜脱离(retinal detachment,RD)复位模型的方法.方法 20只成年健康有色兔(40只眼)随机分为正常组和模型组,每组10只.模型组兔眼用自制的注射针头直视下经玻璃体腔在视网膜下注入透明质酸钠造成视网膜脱离,术后观察眼底情况2～4周.结果模型组10只兔有19只眼成功建立视网膜脱离模型,12～14天后,有18只眼视网膜复位.结论此法可以快速有效地建立一致性较强的视网膜脱离复位动物模型,有助于研究视网膜脱离及其复位的病理改变,影响因素及其治疗方法.     

保持前房状态下小梁切除术的临床观察

目的　探讨保持前房状态下小梁切除术的效果。方法　 77例 ( 88眼 )原发性闭角型青光眼分为两组 :透明质酸钠组 3 2例 ( 4 1眼 )行前房注入透明质酸钠后再行常规小梁切除术 ;对照组 45例 ( 4 7眼 )行常规小梁切除术。结果　浅前房发生率透明质酸钠组 12 2 0 % ( 5 /4 1) ,对照组 3 1 91% ( 15 /4 7) ,两组比较P <0 .0 5 ,差异有显著性意义。手术成功率透明质酸钠97 5 6% ( 4 0 /11) ,对照组 89 3 6% ( 4 2 /4 7) ,两组比较P >0 .0 5 ,差异无显著意义。结论　保持前房状态下小梁切除术可降低浅前房发生率。     

药物联合物理疗法治疗MGD所致干眼的疗效观察

目的：比较左氧氟沙星眼凝胶与妥布霉素地塞米松眼膏治疗睑板腺功能障碍(MGD)所致的蒸发过强型干眼的疗效。

方法：将确诊为MGD所致干眼(蒸发过强型)180例360眼患者随机分组。A组应用左氧氟沙星眼凝胶+玻璃酸钠滴眼液治疗; B组采用妥布霉素地塞米松眼膏+玻璃酸钠滴眼液治疗。A、B两组患者均采用综合治疗：用棉棒挤压排出阻塞在睑板腺管内的分泌物,每周1次,连续4wk为一个疗程; 热毛巾湿热敷眼睑,水温45℃左右,每天3次,每次15min,使残留在睑板腺管内的油脂稀薄软化脂质排出。每次眼睑湿热敷完毕后,A组将左氧氟沙星眼凝胶滴入结膜囊内并涂抹在眼睑睫毛根部,B组将妥布霉素地塞米松眼膏涂抹在眼睑睫毛根部,两组患者均滴玻璃酸钠滴眼液每天4次。观察患者术后的疗效。

结果：两组患者治疗后,临床症状评分、分泌物性状评分、泪膜破裂时间评分和泪液分泌试验评分差异均无统计学意义(Z_症状=-0.64,P_症状=0.524; Z_分泌物=-1.37,P_分泌物=0.171; Z_破裂时间=-1.06,P_破裂时间=0.288; Z_分泌时间=-1.06,P_分泌时间=0.288)。角膜荧光染色评分：A组治愈83.3%,好转11.1%,无效5.6%; B组治愈55.6%,好转27.8%,无效16.7%; A组疗效优于B组(Z=-4.02,P<0.001)。

结论：治疗MGD所致的蒸发过强型干眼,以物理疗法为主,药物治疗为辅,除了患病时间长、病情严重的患者可短期使用妥布霉素地塞米松眼膏外,一般均使用左氧氟沙星眼凝胶完全能达到消炎抗菌、安全稳定、无毒副作用的治疗效果,二者药物之间在治疗效果没有明显的差异。     

玻璃酸钠治疗髋关节骨性关节炎的临床疗效

目的观察玻璃酸钠注射液治疗髋关节骨性关节炎的临床疗效。方法髋关节骨性关节炎患者36例，治疗组18例在关节腔内注射玻璃酸钠2ml，每周1次，5次为1疗程。结果治疗组治疗前VAS评分≥7．0，治疗后6个月时VAS评分为（2．75±0．06），差异有统计学意义（P〈0．05）；6个月时总有效率高达89％（16／18例）。结论玻璃酸钠注射液治疗髋关节骨性关节炎疗效确切、持久，关节功能改善良好。