收费全文 | 126篇 |
免费 | 10篇 |
国内免费 | 1篇 |
儿科学 | 1篇 |
妇产科学 | 2篇 |
基础医学 | 43篇 |
口腔科学 | 3篇 |
临床医学 | 4篇 |
内科学 | 23篇 |
皮肤病学 | 14篇 |
外科学 | 10篇 |
综合类 | 6篇 |
预防医学 | 1篇 |
眼科学 | 2篇 |
药学 | 25篇 |
肿瘤学 | 3篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 5篇 |
2016年 | 5篇 |
2015年 | 2篇 |
2014年 | 5篇 |
2013年 | 10篇 |
2012年 | 6篇 |
2011年 | 2篇 |
2010年 | 8篇 |
2009年 | 5篇 |
2008年 | 4篇 |
2007年 | 8篇 |
2006年 | 10篇 |
2005年 | 10篇 |
2004年 | 11篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 9篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1994年 | 1篇 |
Background
Rosacea is a chronic inflammatory skin condition whose etiology has been linked to mast cells and the antimicrobial peptide cathelicidin LL-37. Individuals with refractory disease have demonstrated clinical benefit with periodic injections of onabotulinum toxin, but the mechanism of action is unknown.Objectives
To investigate the molecular mechanism by which botulinum toxin improves rosacea lesions.Methods
Primary human and murine mast cells were pretreated with onabotulinum toxin A or B or control. Mast cell degranulation was evaluated by β-hexosaminidase activity. Expression of botulinum toxin receptor Sv2 was measured by qPCR. The presence of SNAP-25 and VAMP2 was established by immunofluorescence. In vivo rosacea model was established by intradermally injecting LL-37 with or without onabotulinum toxin A pretreatment. Mast cell degranulation was assessed in vivo by histologic counts. Rosacea biomarkers were analyzed by qPCR of mouse skin sections.Results
Onabotulinum toxin A and B inhibited compound 48/80-induced degranulation of both human and murine mast cells. Expression of Sv2 was established in mouse mast cells. Onabotulinum toxin A and B increased cleaved SNAP-25 and decreased VAMP2 staining in mast cells respectively. In mice, injection of onabotulinum toxin A significantly reduced LL-37-induced skin erythema, mast cell degranulation, and mRNA expression of rosacea biomarkers.Conclusions
These findings suggest that onabotulinum toxin reduces rosacea-associated skin inflammation by directly inhibiting mast cell degranulation. Periodic applications of onabotulinum toxin may be an effective therapy for refractory rosacea and deserves further study. 相似文献Cardiovascular outpatients (n = 170) without taking antihypertensive medication were recruited.
An ELISA for chymase-dependent angiotensin II-forming activity in CML was established using Nma /Dnp-modified angiotensin I.
Logistic regression analysis revealed that age and male gender were significant independent determinants of the increased CML chymase activity. After adjustment by age and gender, the CML chymase activity was positively correlated with systolic blood pressure, pulse rate, and the brain natriuretic peptide level.
The relation between blood pressure and CML chymase activity suggests that it might reflect that increased tissue chymase activity contributes to systemic high blood pressure and heart rate because plasma chymase is inactive due to inhibitory plasma inhibitors. 相似文献